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Showing: 181 - 200 of 694 results

  1. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ... find themselves faced with a roadblock. In this post, we will provide an overview of some of the common... tips on lentiviral titering please see our blog post. Between choosing the best lentiviral system, producing... to check out the lentiviral protocols and blog posts on Addgene’s website for additional resources. ...

  2. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...Send plasmids to Addgene  Quality control  In this post, we’ll explain how to use our deposit spreadsheet...“How to Deposit Your Plasmids with Addgene” blog post for a thorough guide to our online data entry process...discoveries with scientists around the world. This post was originally written by Tyler Ford in March 2017...

  3. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...initiation, elongation, and termination. In a previous post we discussed the promoter's role in the initiation...ultimately gene-expression. Polyadenylation is the post-transcriptional additional of multiple adenine (...Cloning Techniques Read all of our Plasmids 101 Posts Resources on Addgene.org Browse our Plasmid Reference...Trono D, Hope TJ. Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element Enhances Expression of...

  4. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...This post was contributed by guest blogger Derek Simon. The surgeries and standard molecular neuroscience...excitation spectrum (use the resources discussed in post one of this series already provided or check the... to the fun stuff: the experiments! In our final post in this primer on optogenetics we’ll delve into ...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...

  5. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...This post was contributed by Dora Farkas of www.FinishYourThesis.com and is one of many posts in our ...career articles on our blog Read Joanne Kamen's Post on What Makes a Good Mentor Resources on the Addgene...the needs of other people. Some students and postdoctoral fellows are hesitant about expressing their ...

  6. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...This post was contributed by Nikolai Braun and Keira Havens, co-founders of Revolution Bioengineering...Biophysics from UC Davis in 2007. Nikolai trained as a post-doc at the Biochemistry Department at the University...Terriers. More SynBio Reading Read more Addgene blog posts about synthetic biology ...was facing the prospect of having to do a third postdoc in order to rebuild my resume, so I had started...

  8. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ... a team. As described here in a 2012 Intuit blog post, a study coming out of MITs Human Dynamics Laboratory...effective delegation will be covered in a future post in this series). Members carry on back-channel...

  9. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...productivity in 2014. Tip 1: Use color coding, labels, and post-its to make finding your most used protocols & references...wild with sticky notes and labels. Color code your Post-its and write brief descriptions on each. Place ...

  10. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...significant pairings, additional tests, referred to as post-hoc analysis, must be performed. These will be discussed... choosing an ANOVA/MANOVA statistical test   Post hoc tests Knowing that significance exists somewhere... multiple groups is rarely enough information. A post hoc test, done after your initial analysis can provide...ANOVAs/ANCOVASs must be performed first, and then post hoc tests can be run on these tests. All sample...

  11. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...This post was contributed by guest blogger Nathaniel Roquet, a PhD student in the Harvard Biophysics ...researcher in the Lu Lab at MIT. Note: The following blog post reduces the content of our paper, “Synthetic recombinase-based...they can control recombinase activity. For example, post-transcriptional recombinase activation via RNA toehold...Student to SynBio Entrepeneur  Read All of Our SynBio Posts Additional Resources on Addgene.org Visit Our ...

  12. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...updated on Oct 1, 2020 by Aliyah Weinstein. This post was contributed by guest bloggers, Wenning Qin and... this process check out our Mouse Modeling blog posts: Part 1: Genetically Engineered Mice and Part 2:...50% when using ESC (Cohen, 2016). See Our Recent Post for Tips on CRISPR Gene Editing in Mice Disadvantages...additional advice from John Doench’s gRNA design blog post. Finally, always remember that you are working with...

  14. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    ... of another academic year, graduate students and post-docs alike are wondering where the time has gone....2015.04.025. PubMed PMID: 26046646. Related blog posts: Extracurricular Activities for a Strong Science...planning help alleviate the anxiety of graduate and postdoc training. On the other side, the PI has an opportunity...

  15. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...cleavage sites should one need to remove the tag post-purification. A sample of pCri constructs: pCri...to glycosylate proteins and may recapitulate the post-translational modifications added to proteins in...

  17. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ... for fun and life outside of the lab? This final post in the PI Interview Series investigates how these...Maintain a Good Work-Life Balance? Read the Previous Posts in this Series Advice for Choosing a Research Project...

  18. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...expression in transduced cells whenever possible. This post will describe our workflow for viral genome sequencing...quality control process! The images in this blog post were created using Snapgene and Geneious software...Featured Topic Page Subscribe to Viral Vectors Blog Posts Learn How We Use NGS for Plasmid Quality Control...

  19. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...vectors? Browse our Viral Vectors 101 blog posts Read blog posts about neuroscience Resources on addgene.org...increased potency. Download our chemogenetics poster! This blog post was originally written by Maya Peters Kostman...

  20. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...pyrimidine bases (C and T). (Check out our previous post on these editors for more details on how they work...problematic in the development of CBEs and ABEs. This post includes a lot of acronyms, so we’ve included a ...story still isn’t over! Keep an eye out for our next post about base editing, where we’ll tackle transversion...Glossary We introduced a lot of terminology in this post! Here’s a quick refresher if you ever lose track... glycosylation to create an abasic site. In this post, it refers to the same approach as a CGBE. BER...
Showing: 181 - 200 of 694 results