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Showing: 181 - 200 of 521 results

  1. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...sequencing or other readout. Cellular memory is accomplished by the use of sequence-specific enzymes (e.g...also available, N1-LOC8R.  These plasmids are unpublished Unnatural amino acid incorporation in mammalian...detection of neuronal action potentials. The Cohen lab wished to improve on the Arch indicator and their collaborators... that their sgRNA-RNA fusions were functional, Shechner et al used the constructs in their study to target...contiguous with the sgRNA core (see image above). Shechner, et al. Nat Methods. 2015. PubMed PMID: 26030444...regulation in live cells since their introduction by Sherf and Woods in the nineties. However, reliance on ...guess what? pXPG is now available at Addgene. Sherf, et al. 1994. Promega Notes Mag. Giannakis, et...

  2. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...system (PNS). (Capsids, if you recall, are the outer shell of an AAV vector, which determine the vector’s tropism...Addgene’s Data Hub and the CLOVER repository of published systemic AAVs to find commonly used vector cargo...capsid, while Table 1 gives a brief overview of published descriptions of commonly used or emerging capsids... plasmids available from Addgene Capsid Published Tropism Model Organism Mouse Strain-Specific...Caltech’s CLOVER Center’s extensive repository of published systemic AAV experiments and Addgene’s AAV Data...your validation data to our Data Hub once you’re finished!) Good luck and happy transducing! This post was...

  3. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ... SNAPf. eDHFR: The Virginia Cornish and Michael Sheetz labs demonstrated in 2005 that proteins fused with...j.chembiol.2019.01.003 Gallagher, S. S., Sable, J. E., Sheetz, M. P., & Cornish, V. W. (2009). An In Vivo Covalent...doi.org/10.1021/cb800025k Miller, L. W., Cai, Y., Sheetz, M. P., & Cornish, V. W. (2005). In vivo protein...10.1038/s41467-023-40788-8 Ulrichs, H., Gaska, I., & Shekhar, S. (2023). Multicomponent regulation of actin ...

  4. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...genes across the genome. Although this tool was published in 2011, it was only recently made available through...outer mitochondrial membrane. Sequestration is accomplished by knocking down your endogenous protein of ...shRNA. This system requires less work than using established methods and minimizes some of the technical issues...the CRUSH vector in transgenic mice, the lab established a dual-color RNAi “sensor” mouse strain in which...and involve many steps. The Drew Endy and Keith Shearwin labs have developed a new, streamlined approach...

  5. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ...Zhang’s lab recently developed a platform called SHERLOCK (specific high-sensitivity enzymatic reporter ...more. Read the paper in Science Find the new SHERLOCK plasmids More precision with dual-nuclease Cas9...our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide...

  6. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ... LaManna, published Mar 8, 2016. The updated and expanded version by Nyla Naim was published Sept 3, 2020...dCas9 Initial photoactivatable CRISPR systems published in early 2015 focused on using light to control... and repeatable. Since these initial studies published in 2015, more split systems have been developed...

  7. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...world. 4)      The Annals of Internal Medicine published systematic reviews on an agent used to promote... into account. 7)     Science’s John Bohannon published an article describing a sting operation in which...submitted a phony paper to a list of 304 open access publishers. By the time the magazine went to press, no less...

  8. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...listed in the table has been used effectively in a published article. This blog post will serve as a guide ...deposited gRNA plasmids or from submission of a spreadsheet containing the validated gRNA sequences alone... and we’ll add them to this list. Access the spreadsheet for submitting your gRNA sequences here. Features...

  9. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...better for you) and place them on an easy to reach shelf. Make sure all notebooks are visibly labeled on ...you’re looking for. Tip 3: Share protocols and spreadsheets with lab members Now that you’ve organized your... may also want to consider moving your paper spreadsheets for reagent storage locations, such as the list...

  10. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...microbial DNA from groundwater. Now, they’ve recently published data demonstrating that CasX is active in both...for cancer research The Sidi Chen lab recently published two CRISPR knockout libraries for cancer research...our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide...

  11. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...Originally published Mar 3, 2016 and last updated Apr 13, 2021 by Will Arnold. Although CRISPR systems...E. coli and T. citrea scarless editing plasmids Sheng Yang’s lab describes a two-plasmid system that combines...the above CRISPRi section, the Marraffini Lab published a robust tool for CRISPRi and CRISPRa using RNAP-Omega-dCas9...systems is base editing. This process is generally accomplished by fusing an enzyme capable of converting one...

  12. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...Coroadinha, 2006) and retroviral production systems (Shen, 2011). To our knowledge the effect of hyperosmolarity...37.10 (1991): 989-993. PubMed PMID: 18597326. 5. Shen, Chun Fang, and Amine Kamen. "Hyperosmotic pressure...22154564. 6. Stoneham, Charlotte A., Michael Hollinshead, and Amin Hajitou. "Clathrin-mediated endocytosis...

  13. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...editing and prime editing approaches. Recently, Xue Sherry Gao’s lab at Rice University has developed DAP ...tRNAs Luckily, a fun feature of tRNA was fully unleashed by the Gao Lab’s work. Led by PhD student Qichen... Qichen Yuan is a PhD student in the lab of Xue Sherry Gao at Rice University.    References and Resources...

  14. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ... is universally useful. 4. Chemical Safety Data Sheets by ThatsMyStapler Safety always comes first with...including this MSDS app. The Chemical Safety Data Sheets app has a search function that allows the user ...Restriction Enzyme Tool by Promega Material Safety Data Sheet by RSS-ICTD Amino Acid Structure Quiz/Card by Andrey...

  15. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...Sonication provides a truly random digestion, but is harsher and requires more optimization. Enzymatic digestion...more of the protein:DNA complexes. Once you’ve finished fragmenting the DNA, spin down the samples to ... Schematic overview of ChIP. Image courtesy of Shengliu via Wikicommons.   Okay, let’s do a quick ...

  17. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ... question. Three different labs have recently published work that provides a solution to this problem....genomic deletions In PRIME-del, developed by Jay Shendure’s Lab, paired pegRNAs work with the PE2 enzyme ...our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide...

  18. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...those tips for you in one place! First, a quick refresher: prime editing is a “search and replace” genome...j.cell.2021.09.018 Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A., Chen, P. J., Anzalone, ...Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., Zhang, F., & ...

  20. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ... an increase in the complexity. In 2018, they published a paper profiling four base overhang ligation ... to generate high-fidelity reactions. (Need a refresher? The five rules are: (1) don’t use the same overhang...fragments may be near the upper limit of what can be accomplished with Golden Gate Assembly—even when getting ...
Showing: 181 - 200 of 521 results