We narrowed to 219 results for: ttl

1. Designing Your Chalk Talk for the Academic Job Interview

   Type

   Blog Post

   Published

   March 12, 2019, 1:37 p.m.

   ...eating one before your chalk talk. Bring your own bottle of water. If possible, write down your short aims...

2. Viral Vectors 101: Preparing Pooled Libraries

   Type

   Blog Post

   Published

   July 13, 2023, 1:15 p.m.

   ...pooled library from Addgene, you may have been a little surprised at how this powerful tool is in such ...

3. Rett Syndrome: A History of Research and Therapeutic Outlooks

   Type

   Blog Post

   Published

   Nov. 19, 2020, 2:15 p.m.

   ...much of the MECP2 protein is as detrimental as too little, resulting in MECP2 duplication syndrome or Rett...

4. Overcoming the AAV Size Limitation for CRISPR Delivery

   Type

   Blog Post

   Published

   Sept. 16, 2020, 1 p.m.

   ...this approach has been proven feasible, it leaves little room for additional regulatory elements. Feng Zhang's...

5. The PAM Requirement and Expanding CRISPR Beyond SpCas9

   Type

   Blog Post

   Published

   Aug. 20, 2020, 2:30 p.m.

   ...Ibraheim R, Gupta A, Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, ...

6. Plasmids 101: Plasmid Incompatibility

   Type

   Blog Post

   Published

   March 31, 2020, 1:15 p.m.

   ...partitioning (Schumacher, 2012). This concept is a little more complex than simply competing for replication...

7. An Inside Look into Addgene's Dev Team

   Type

   Blog Post

   Published

   Jan. 23, 2020, 2:45 p.m.

   ... I enjoy doing code reviews because it’s like a little peek into the mind of the developer and I get to...

8. Antibodies 101: Reading a Flow Plot

   Type

   Blog Post

   Published

   Feb. 6, 2024, 3:13 p.m.

   ...reported from flow cytometry experiments can be a little challenging to understand if you’ve never done ...

9. Transferable Skills Guide: External Collaborations

   Type

   Blog Post

   Published

   April 19, 2018, 1:25 p.m.

   ...scientific field. Reaching out to people who have little in common with you is much harder than starting...

10. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type

    Blog Post

    Published

    Dec. 14, 2017, 2:08 p.m.

    ... Postdoc at Massachussetts General Hospital. A little over a decade back when Yamanaka and colleagues...

11. How to Write a Scientific Review Article

    Type

    Blog Post

    Published

    Feb. 16, 2017, 3:30 p.m.

    ...real time as I write. Things are going to get a little crazy (meaning you are probably going to cite hundreds...

12. Antibiotic Resistance: An Old Solution but a New Problem

    Type

    Blog Post

    Published

    Nov. 10, 2016, 3:30 p.m.

    ...with compounds similar to those they have been battling for centuries. As such, resistant populations ...

13. Using Video to Share Your Science: We Share Science

    Type

    Blog Post

    Published

    Nov. 3, 2016, 2:30 p.m.

    ...scientific journals flies under the radar and gains little notice. A 2009 research study found that 12% of...

14. Important Considerations in Optogenetics Behavioral Experiments

    Type

    Blog Post

    Published

    Oct. 1, 2015, 2:30 p.m.

    ...as well. Inhibition of neuronal activation is a little more complicated since basal neuronal activity ...

15. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type

    Blog Post

    Published

    Aug. 5, 2014, 6:51 p.m.

    ...searching and extraction of information. Looking a little further ahead, journals may move towards encouraging...

16. Plasmids 101: The Promoter Region – Let's Go!

    Type

    Blog Post

    Published

    April 3, 2014, 8:05 p.m.

    ...etc) require unique promoters and there is very little crossover. Generally speaking, promoters in bacteria...

17. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type

    Blog Post

    Published

    April 4, 2024, 1:15 p.m.

    ...principles of gating, you’ll first need to learn a little bit of theoretical background on the different ...

18. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    Published

    Jan. 26, 2023, 2:15 p.m.

    ...modifications (~1-50 base pairs). Small edits require as little as 30-50 bases for each homology arm, but keep ...

19. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    Published

    Sept. 1, 2020, 1:15 p.m.

    ... ML, Agbandje-McKenna M, Jude Samulski R (2010) Little Vector, Big Gene Transduction: Fragmented Genome...

20. CRISPR 101: Validating Your Genome Edit

    Type

    Blog Post

    Published

    Nov. 3, 2022, 12:15 p.m.

    ...(the 3rd sequencing trace). While it requires a little extra wet lab work, this is still a great option...