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We narrowed to 245 results for: virus

Showing: 181 - 200 of 245 results
  1. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...Gallagher, T. L., & Gao, G. (2024). Adeno-associated virus as a delivery vector for gene therapy of human diseases... while both AAV and lentivirus have low immunogenicity, AAV still beats lentivirus. In mice, AAV is almost...made at high titers equal to those achieved with lentivirus but offer an improved safety profile, customization...expression in many mouse tissues. Safety AAV and lentivirus have different safety profiles that often affect...experiments. AAV can be safely handled at BSL-1, while lentivirus requires a BSL-2 setup. This requires a less ...unmatched by other viral vector delivery methods. Lentivirus has limited packaging options, with most lentiviral...the CRISPR components — are expressed. Unlike lentivirus, cargo delivered by AAV does not integrate into...
  2. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    .... One of the best optimized is the tobacco etch virus (TEV) protease. A TEV protease cleavage site is ...
  3. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ... in originality. It may not be easy to optimize virus production with roller skates—though I could get...
  4. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...Griffin. "Development and characterization of sindbis virus with encoded fluorescent rna aptamer spinach2 for...
  5. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...professors, stopping by to say, “Everything I know about virus, I learned from Addgene,” or “I learned to clone...Lentiviral Guide AAV Guide Optogenetics Guide γ-Retrovirus Guide Protocols Agarose Gel Electrophoresis...help users get started with tools like CRISPR, lentivirus, and optogenetics — and shared the protocols ...
  6. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...and Daniel Nathans. "Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus...
  7. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...potential to overcome some of the limitations of viruses. TEs have a large payload, up to 100 kB with Sleeping...into new target sites. This is similar to how retroviruses, like HIV, replicate. Class I TEs do not encode...(non-LTR TEs). LTR retrotransposons are like retroviruses both in their structure and mechanism of replication... (~5 kB payload for AAV and ~8 kB payload for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin...
  8. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...and information on hardware, optogenetics tools, viruses, and more.  Conclusion   Optogenetic tools provide...
Showing: 181 - 200 of 245 results