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We narrowed to 243 results for: virus

Showing: 181 - 200 of 243 results

  2. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...relatively laborious because multiple plasmids/viruses are needed to deliver all the protein and RNA components...Cas9 activators can be delivered to the CNS by lentivirus, which has a larger packaging capacity than AAV...vivo CRISPRa (Savell et al., 2019). This dual lentivirus system pairs ubiquitous expression of sgRNA(s... such as EF-1α and CAG. Figure 2: Dual lentivirus system with ubiquitous expression of sgRNA (top...

  4. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    .... One of the best optimized is the tobacco etch virus (TEV) protease. A TEV protease cleavage site is ...

  6. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...professors, stopping by to say, “Everything I know about virus, I learned from Addgene,” or “I learned to clone...Lentiviral Guide AAV Guide Optogenetics Guide γ-Retrovirus Guide Protocols Agarose Gel Electrophoresis...help users get started with tools like CRISPR, lentivirus, and optogenetics — and shared the protocols ...

  7. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ... in originality. It may not be easy to optimize virus production with roller skates—though I could get...

  10. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...Griffin. "Development and characterization of sindbis virus with encoded fluorescent rna aptamer spinach2 for...

  12. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...and Daniel Nathans. "Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus...

  17. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...and information on hardware, optogenetics tools, viruses, and more.  Conclusion   Optogenetic tools provide...

  19. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...potential to overcome some of the limitations of viruses. TEs have a large payload, up to 100 kB with Sleeping...into new target sites. This is similar to how retroviruses, like HIV, replicate. Class I TEs do not encode...(non-LTR TEs). LTR retrotransposons are like retroviruses both in their structure and mechanism of replication... (~5 kB payload for AAV and ~8 kB payload for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin...
Showing: 181 - 200 of 243 results