We narrowed to 245 results for: virus

... use qPCR with a standard curve to titer their viruses; food allergy labs use standard curves with ELISAs...

...relatively laborious because multiple plasmids/viruses are needed to deliver all the protein and RNA components...Cas9 activators can be delivered to the CNS by lentivirus, which has a larger packaging capacity than AAV...vivo CRISPRa (Savell et al., 2019). This dual lentivirus system pairs ubiquitous expression of sgRNA(s... such as EF-1α and CAG. Figure 2: Dual lentivirus system with ubiquitous expression of sgRNA (top...

... has been used to differentiate strains of Zika virus, genotype human DNA, identify tumor mutations within...

...one bacterium to another through bacteriophage (viruses that only infect bacterial cells), or a bacterium...

.... One of the best optimized is the tobacco etch virus (TEV) protease. A TEV protease cleavage site is ...

...professors, stopping by to say, “Everything I know about virus, I learned from Addgene,” or “I learned to clone...Lentiviral Guide AAV Guide Optogenetics Guide γ-Retrovirus Guide Protocols Agarose Gel Electrophoresis...help users get started with tools like CRISPR, lentivirus, and optogenetics — and shared the protocols ...

...SpCas9, so it can be packaged into adeno-associated virus (AAV).  At 984 amino acids in length, Cas9 from ...

...phenotypes in diverse plants, animals, microbes, viruses, and fungi for which these methods are presently...

... in originality. It may not be easy to optimize virus production with roller skates—though I could get...

...culture-free heritable genome editing using RNA virus-based delivery in wheat. Nature Plants. https://...

...Griffin. "Development and characterization of sindbis virus with encoded fluorescent rna aptamer spinach2 for...

..., Trader, C., . . . Tasic, B. (2021). Enhancer viruses for combinatorial cell-subclass-specific labeling...

...and Daniel Nathans. "Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus...

...Addgene.org Find Plasmid Cloning Protocols Find Virus Protocols Find Basic Molecular Biology Protocols...

...improved due to the addition of a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), ...

...mouse embryos using recombinant adeno-associated viruses. Nat Commun 9: . https://doi.org/10.1038/s41467...

...accomplished with transfections and are therefore virus-free. This is achieved using the piggyBac™ transposase...

...and information on hardware, optogenetics tools, viruses, and more.  Conclusion   Optogenetic tools provide...

...potential to overcome some of the limitations of viruses. TEs have a large payload, up to 100 kB with Sleeping...into new target sites. This is similar to how retroviruses, like HIV, replicate. Class I TEs do not encode...(non-LTR TEs). LTR retrotransposons are like retroviruses both in their structure and mechanism of replication... (~5 kB payload for AAV and ~8 kB payload for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin...

...-villain geneticist named Zobrist who designs a virus that can potentially kill one-third of the human...