We narrowed to 559 results for: ESP

...always a good idea to perform primer optimization, especially if you are having difficulty amplifying your ...colonies, check that the antibiotic in the plate correspond to the antibiotic resistance marker present in...small culture volume (2 or 3 ml) containing the corresponding antibiotic, extract the plasmid, and analyze...

...scientific publishing has a number of drawbacks, especially the increasingly growing time from submission... a few individuals.  Early-career researchers especially enjoy the ability to post a preprint as evidence...editors’ comments, reviewers’ comments, and author responses can be shared directly alongside the preprint ...

...stimulates the overall immune response. Once the initial antibody response starts to wane about a month...include any antibodies produced during the immune response.   Polyclonal antibodies are generated ...

...everything. If it’s barcoded, it should have a corresponding entry in your database. Enjoy your happy lab...at some point, decide early on what to do in a namespace collision. It may even be worth it to have extra... you're solving, but consistency is key. Some namespace collisions you may encounter: vector, sequence...

...cyclization recombinase”), is a 38 kDa protein responsible for intra- and inter-molecular recombination ...lox system is a well-established research tool, especially in the field of mouse transgenics. A few of the... (FRT) site being analogous to Cre and loxP, respectively. The FLP-FRT technology can be an effective ...

... using AdV is that it triggers a strong immune response after in vivo administration, which results in...expression ceases relatively quickly due to immune response against AdV. Importantly, recombination-competent...spectrophotometry. An absorbance of at 260nm corresponds to 1.1 x 1012 particles/mL. To calculate the ...

...lack all viral genes, including those that are responsible for integration into host chromosomes, further... (HDR). This characteristic makes AAV vectors especially amenable to correcting disease-associated mutations...PMCID: PMC4450362. 4. Sun, J. Y., et al. "Immune responses to adeno-associated virus and its recombinant ...

... and AsCpf1 are 3.7 kb and 3.9 kb in length, respectively, larger than SaCas9 but smaller than SpCas9....These nucleases should fit well into AAV vectors, especially given that Cpf1 gRNAs are also shorter than those...rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins...

...communication and learning to work with people despite the distance improved my verbal and written communication...strengths and work effectively. Leadership responsibilities Being a leader in an organization, you need... different hats and came to understand the responsibilities that come with leadership. The NPA was a great...

... the spotlight. TnpBs are gaining popularity, especially among plant biologists, due to their compact ...Pre-existing stem cell heterogeneity dictates clonal responses to the acquisition of leukemic driver mutations... ex vivo cell engineering applications and is especially well-suited for lung cells via airway administration...