...showed that nanobodies could be fused to other proteins, and these fusions retained the ability to bind ...Caussinus, E., Kanca, O., and Affolter, M. “Fluorescent fusion protein knockout mediated by anti-GFP nanobody....

...replace” genome editing technique that relies on a fusion enzyme of Cas9 nickase + reverse transcriptase ...Nelson et al., 2022). PE7: This editor includes the fused RNA-binding protein La to protect the end of the... not epegRNAs) can improve either endogenous or fused La binding (Yan et al., 2024). Figure 9:...

...our FRET Resource page and can be used to create fusions with a gene of interest. These plasmids will be..., where the donor and acceptor fluorophores are fused to two separate proteins. (In contrast, intramolecular...achieve, because the ratio of acceptor to donor fusions varies with transfection efficiency and any unpaired...

...with Peptide-Assisted Genome Editing (PAGE). They fused the Cas enzyme to cell-penetrating peptides and ...guides. Figure 1: A) Cell-penetrating Cas9, fused to HIV TAT, Myc and SV40 Nuclear Localization Signals... et al. (2023). StayGold variants for molecular fusion and membrane-targeting applications. Nature Methods...

...protein-protein interactions. One popular approach is to fuse a protein of interest to each part of a split fluorescent...General Design Figure 1: Two proteins are fused to the FP(11) and FP(1-10) fragments. When Protein...cell environments). Also consider that a protein fusion may disrupt functionality or binding of your target...

...  Confirming tags and fusion proteins Finally, we confirm promoters, tags, fusion proteins, and selectable...NCBI entry or using BLAST.   Confirming tags and fusion proteins. We’ll break down each of these steps...

...2: CLIP-SNAP fusion proteins for single-molecule FRET. A) Schematic of a CLIP-SNAP fusion protein (a.k.a...YFP-tagged G protein and a NanoLuc® (Nluc) Luciferase-fused detector that specifically binds to active (GTP-...

...co-expressed with a fusion protein containing VP2C and a protein of interest (POI). The VP2C fusion protein anchors...while still allowing substrates and products to diffuse freely into or out of the protein compartment. ...

...Cas break for HR is to fuse an HR protein to the nuclease. Early HR factors fused to Cas9 have been shown..., A. H. Y., Menerot, S., Brion, A., et al. CtIP fusion to Cas9 enhances transgene integration by homology-dependent...

...live cells. Indeed, groups have utilized dCas13 fusion proteins for imaging, tracking, modulating splicing...Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion to stabilize the protein in mammalian cells. LwaCas13a-msfGFP...programmable RNA-binding protein, creating the fluorescent fusion protein dLwaCas13a-NF for in vivo RNA imaging. ...