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We narrowed to 729 results for: NSI

Showing: 101 - 110 of 729 results

  1. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...include a Packaged on Request option. Through this expansion, scientists will be able to request an AAV composed... vector prep.    Excited? So are we! This expansion will give researchers increased flexibility when...overview in this blog post.     When will the expansion happen?  You will be able to request Packaged ... Plasmids available for Packaged on Request consideration will have a banner on their material page. Interested... in-stock preps. If a request has technical considerations that may affect expected titers, a customer...resources.  We hope you’re as excited about this expansion as we are! Happy packaging! ...

  2. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ....” Subscribers to Scientific America would be considered the interested public and it would be safe to...scientific or science communication. A Tweet is still considered scientific communication if the language is aimed...  For example, while “PCR test” has recently transitioned from jargon to non-jargon in the context of ...of disease testing, it can still be considered jargon in most other uses. Many laypeople would use “PCR...Instead, it targets a specific audience - giving consistency, purpose, structure, and intent to the messaging...your audience, and their expectations of you, consistent and clear.   And now that we’re all on the same...

  3. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...aborted fetus. HEKs have an unusual ploidy and are considered hypotriploid: most chromosomes are represented...though derivatives have been adapted for growth in suspension. Of the many derivatives of HEK-293, the most...many immature neuron-like qualities, which is inconsistent with kidney cells (Shaw et al.). Since then,...systems, such as yeast. HEKs can also grow in suspension, which allows for large scalability, and can ...cells may be particularly useful due to their intrinsic properties. HEK-293 cells are tumorigenic, ...widely available, which can make for a seamless transition of biological tools to other researchers using...

  4. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    Published
    March 4, 2014, 4:49 p.m.
    ...describe the discovery of a light-sensitive opsin from algae that is sensitive to red light instead of blue...The problem is that all of these molecules are sensitive to blue light. Many have tried to make green molecules...blue light molecule much, much, much more light-sensitive. Then you can use dim blue light and red light... tools in a deep part of the brain, making it sensitive to light. When they illuminated this region, the...for the first time? Boyden: There are always considerations for each species. This could be a three or ...

  5. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...” has me considering the microbiome more often than not). If you’re someone who’s considering getting ...definitely enjoyed the lab!) and would therefore consider myself a neuroscience novice (newb). In this post...’ve developed in your current work to make new insights in neuroscience. Even if you're currently a synthetic...altered function or maybe your favorite FRET-based biosensing technique can be applied to an important neuronal...experiments and to find tools that will make your transition into the field much easier! If there’s a plasmid...

  6. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ...Transferred DNA once inside the infected bacterium can either exist as transient extrachromosomal DNA,...inject their genetic material into the cell. Once inside, phages can follow one of two different life cycles...Lentiviral and AAV can be used to create both transient cell lines, where a gene of interest is expressed...Bacterial conjugation Conjugation was the first extensively studied method of gene transfer and was discovered...the other hand uses electrical pulses to create transient pores in the cell membrane that genetic material...

  7. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...molecular switches in signal transduction pathways and consist of a trimer of three subunits: alpha (Gα), beta...function as one unit: Gβγ. In general, GPCRs show a propensity to associate with certain subtypes, although ...ligand-bound GPCRs of interest. The TRUPATH plasmid kit consists of 20 plasmids encoding 14 alpha, 2 beta, and ... internalization of two different receptors- angiotensin II type 1 receptor and the β2 adrenoceptor. ...ideal for chemogenetics as they are relatively insensitive to their endogenous ligand, acetylcholine, and...Pascolutti R, Paulo JA, Gygi SP, Kruse AC (2017) Multidimensional Tracking of GPCR Signaling via Peroxidase-...

  8. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...order to gain a competitive advantage this cycle, consider the advice below:  1. Eligibility check Scrutinize...and grades that serve as automated filters of consideration. If you are worried about any eligibility provision...which is the perfect opportunity to paint a comprehensive picture of your academic interests, potential... day of the interview, dress the part, and be consistent with your personal statement, while you make ...your close peers to come along for the ride, and consider applying to several opportunities. In an age of...success. Every student should use these guidelines consistently across a number of years as you apply for internships...

  9. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...Brightness Brightness refers to the fluorescence intensity of a fluorescent molecule. A higher brightness...sample. Practical brightness A protein with a high intrinsic brightness that does not fold well or cannot be...brightness and is of no use. Therefore, it is more sensible to compare fluorescent proteins based on their...wavelength, available emission filters, and detector sensitivity) and the biological system (temperature, prokaryote...brightness When a dish of mammalian cells is transiently transfected with a plasmid containing a fluorescent... there is huge variation in the fluorescence intensities of individual mammalian cells within the dish...

  10. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...and tension all have specific sensors designed for them. For example, this class includes tension monitors..., which will turn on, change color, or change intensity of fluorescence in the presence of calcium. This...specific proteins of interest such as actinin. When tension is high vs. low in the cell the donor and acceptor...changes to the structure of actinin in low vs. high tension. These systems are powerful tools to measure real-time...reproducible when used as stable systems, rather than transiently overexpressing at the time of use. Another great...
Showing: 101 - 110 of 729 results