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  1. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...enhanced random integration efficiencies that are accomplished with transfections and are therefore virus-free...enhance gene editing? Gene editing can now be accomplished in a more cost-effective and efficient manner...

  2. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...tested was constantly tracked with barcodes and spreadsheets. It was great to be in the lab doing experiments...was to fulfill contracts, my work sometimes felt rushed and the focus always seemed more on getting results...

  3. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...Multiple first or corresponding author publications (published, not in preparation) A history of successful funding... meet future collaborators and get access to unpublished information and reagents. Part of the reason ...

  4. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...Originally published Jan 27, 2015 and last updated Dec 17, 2020. This post was contributed by Jordan ...experimentation. In 2014, several manuscripts published in Genetics detailed approaches in which selection...

  5. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...23934178. PubMed Central PMCID: PMC3782611. 8. Tsai, Shengdar Q., et al. "GUIDE-seq enables genome-wide profiling...23503052. PubMed Central PMCID: PMC3651036. 15. Tsai, Shengdar Q., and J. Keith Joung. "Defining and improving...

  6. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...Originally published Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy...plasmids from this paper at Addgene. 5. Tsai, Shengdar Q., et al. “Dimeric CRISPR RNA-guided FokI nucleases...

  7. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...manufacturer and/or in the published literature. Note that the antibody being published doesn’t always indicate...

  8. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...advantage of AAV vectors is that there are well-established protocols for their production and purification...PubMed Central PMCID: PMC4324159. Castle, M.J., Gershenson, Z.T., Giles, A.R., Holzbaur, E.L., and Wolfe...

  9. New Antibodies in the Lab Animation!

    Type
    Blog Post
    Published
    Nov. 19, 2024, 2:15 p.m.
    ...cytometry. It's a great introduction to — or quick refresher on — these common antibody applications.  Thanks...

  10. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    Published
    Sept. 19, 2024, 3 p.m.
    ...is below.      And you’ll be able to find all published videos in our Quick Tips playlist:  Want your...

  11. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...processing of our sequencing data.”  Recently, Seqera published a case study on how Addgene has used Nextflow ...

  16. The Twelve Days of CRISPR

    Type
    Blog Post
    Published
    Dec. 22, 2020, 2:15 p.m.
    ... the second day of CRISPR, Addgene gave to me: SHERLOCK and DETECTR, two CRISPR-based methods to detect...

  17. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...recycling in the event the object in question cannot be washed or reused/repurposed in some fashion. Addgenies...

  20. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ...Instagram all summer long to see what kind of shenanigans we get into!  We hope you are as excited as we...

  21. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    Published
    Jan. 15, 2019, 2:19 p.m.
    ... @EikeMahlandt @Saskia_deMan @AnoeskaMoosdijk @ShebaAJ pic.twitter.com/X6QYXteRLT — Nika Heijmans (@nikaheijmans...

  22. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ...given a DOI for easy citation by others and, when published, will meet FAIR data-sharing guidelines. But ...

  23. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    Published
    Dec. 16, 2014, 8:01 p.m.
    ... What is a plasmid? By Margo R. Monroe Best wishes for your research during this holiday season and...

  26. The Addgene Antibody Data Hub

    Type
    Blog Post
    Published
    Sept. 12, 2024, 1:15 p.m.
    ...completeness. The information is then formatted and published as a report with a DOI for citation. If you’re...

  27. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ... available. I recommend a few: -The recently established Ebola Genome Portal at the University of California...

  28. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ...our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide...

  29. Addgene's Website Update

    Type
    Blog Post
    Published
    April 8, 2024, 2:15 p.m.
    ...features and designs addgene.org! And when you’re finished, feel free to take a moment to let us know what...
Showing: 301 - 330 of 515 results