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We narrowed to 673 results for: plasmid

Showing: 401 - 440 of 673 results

  1. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent Protein Guide...accounted for in your experimental design. Find nLight plasmids here! Next steps While nLightG and nLightR greatly...

  2. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...transfect the ‘helper’ plasmids (viral packaging machinery) along with the viral plasmid containing the cargo...T-antigen. This allows them to replicate viral plasmids with the SV40 origin of replication. To generate...

  3. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...instance, Lifeact-mTurquoise2 (Plasmid #36201) or mScarlet-tubulin (Plasmid #85045). We often use restriction...advisable to first express the fusion protein from a plasmid-based system to assess whether the chimera is still...Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse...protein fusions Browse the fluorescent proteins plasmid kits ...measures homodimerization of FPs targeted to the endoplasmid reticulum based on the appearance of OSER whorls...

  4. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...research? Check out Brainbow plasmids available from Addgene! Find Brainbow Plasmids @Addgene: Brainbow 1.0...1.0, 1.1, 2.1 plasmids Brainbow 3.0, 3.1, 3.2 plasmids References: Transgenic strategies for combinatorial... of Cre-lox. Check out our previous blog post, Plasmids 101: Cre-lox, if you need a quick primer on how...

  5. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ... is well suited for genome editing.  Find Cpf1 Plasmids at Addgene   Image attribution: Zetsche...Biotechnol. 2016 Jun 6. PubMed PMID: 27272384 Find plasmids from this publication at Addgene. 2. Kleinstiver...Biotechnology (2016). PubMed PMID: 27347757. Find plasmids from this publication at Addgene. 3. Zetsche ....cell.2015.09.038. PubMed PMID: 26422227 Find plasmids from this publication at Addgene. 4. Makarova...

  6. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...commonly used CRISPR nuclease. Note: Most CRISPR plasmids from Addgene produce either a gRNA, a nuclease... to deliver. Not all cells efficiently take up plasmids used to produce CRISPR systems    ...

  7. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...moved a gene required for phage propagation to a plasmid that lacked a promoter — only CAST integration ...integrate DNA payloads of up to 15 kb. Find evoCAST plasmids here! The new horizons of evoCAST In the emerging...Addgene's CRISPR Guide (Jump to Large Edits) CAST Plasmid Collection ...

  8. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...Kotton Learn How Plasmid Sharing Speeds Science Resources at Addgene.org Order Plasmids from Addgene! ...to engineer microbes. We’ve ordered a few yeast plasmids from Addgene and are eager to do our first yeast...

  9. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...enough. For example, if we need to develop a new plasmid collection page, we certainly don't expect that...design every icon, curate all of the appropriate plasmids, and code the entire page. That would be entirely...Addgene Website Are You a Molecular Biologist? Find Plasmids for Your Research Here!        ...

  10. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ... usage bias in Plasmids 101: Codon usage bias Read more about plasmids in our Plasmids 101 blog series...Reference Read about genetic code expansion Browse our plasmid collection page  ...

  11. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...popular split-FP plasmids from our catalog to help you find the right split-FP plasmid for your experiment...below, and click the Article link to find all the plasmids from the associated paper:   Color Base FP...and experimental design. We hope you find these plasmids useful. Good luck, and happy experimenting! References...

  12. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...resources on addgene.org Find Optogenetics Plasmids Find Biosensor Plasmids Visit the Fluorescent Protein Guide...Frank Hayward et al., 2024). They deposited their plasmid with Addgene, hoping to inspire others to explore...Lab is eager to hear from you. Find the MagLOV plasmid here!   References and resources References Duret...

  13. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...transforming both fusion plasmids, or mating two single haploid yeast fusion plasmid transformants into a ...reporter system (e.g. the Gateway-compatible bait Y2H plasmid pEZY202  from Yu-Zhu Zhang’s lab) is an example...Yeast Expression Vectors Find Bacterial Two Hybrid Plasmids Read our Blog Post About Yeast Vectors Read our...

  14. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...Addgene.org Browse our CRISPR plasmids for visualization Find plasmids for your research Read our CRISPR..., give CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References Ma, Hanhui et al. “Multicolor...

  15. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...-mediated cloning CRISPR plasmids: Yeast Yeast Synthetic Biology Plasmids ...the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors Tips for Screening with the...

  16. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...that Addgene’s mission statement extends beyond plasmids, AAVs, and antibodies to our content, website,...mission is to enable that process. Our repository of plasmids, AAVs, antibodies, and data allows scientists ...Resources on the Addgene Blog Science careers Plasmids CRISPR Viral vectors Fluorescent proteins  ...

  17. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...and depositors — each with subcategories such as plasmids and viral preps for catalog, blog posts and protocols... and category. For example, a search scoped to plasmids shows filters for expression and vector type, ...related items (for example, a kit that contains a plasmid with the catalog ID). Depositor suggestions provide...

  18. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...Addgene Wesbite Find Reprogramming Plasmids Find Other Hot Plasmid Tools Visit Our Stem Cell Pages ...gratifying to see that Addgene has ensured that plasmid acquisition is not a rate-limiting step in stem...

  19. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ...FITS deposit also includes pLX_305_Ovalbumin, a plasmid that enables introduction of the model antigen,...to study antigen-specific responses. Find FITS plasmids here! Marty LaFleur is a Postdoctoral Fellow in...

  20. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...Browse fluorescent protein plasmids and resources See all Jaffrey lab plasmids ... cellular processes by transforming cells with plasmids encoding aptamer-tagged RNA and later introducing...aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores describes the enrichment...

  21. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...their MECP2-related plasmid tools with Addgene and share additional resources. Plasmid tools to facilitate...to partner with them to launch a Rett Syndrome Plasmid Collection and Resource Center for Rett Syndrome... Rett Syndrome in novel ways.  Addgene’s MECP2 Plasmid Collection and Resource Center will continue to...

  22. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...vectorization strategies include creating an AdV transfer plasmid with the E1 and E3 (Early 1 and Early 3) genes .... These genes’ functions are rescued by helper plasmids used during production and/or by genes present...made available by this deletion from the genomic plasmid can be used to house a recombinant sequence (transgene...late genes are usually present in the transfer plasmid for the majority of adenoviral vector systems used...

  27. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...s incredible.” Looking for CRISPR Plasmids? CRISPR-Cas9 Plasmids available at Addgene CRISPR-Cas9 References...

  28. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...these diseases and disorders. You can find the plasmids for FlipGFP and FlipCherry at Addgene. References...Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse...protein fusions Browse the fluorescent proteins plasmid kits ...

  29. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...Fluorescent protein plasmids Use FRET to study protein-protein interactions Deposit plasmids with Addgene! ...Tagged protein can easily be overexpressed from a plasmid but overexpression may interfere with the system...

  30. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...Chudakov et al, 2007). We have developed a variety of plasmids that can be used in the OSER assay including CytERM-mKOkappa...documenting it in some other way. Besides the data, new plasmids and/or cells or organisms that are engineered ...Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire...

  31. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...their required components are very similar. While plasmids containing virus-specific elements such as the...integrate, and the addition of a helper virus or plasmid allows it to replicate episomally, essentially ...-associated Virus (AAV) Guide Addgene’s viral plasmids and resources Addgene’s viral services: AAV ...

  33. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    Published
    Nov. 25, 2014, 4:02 p.m.
    ...come to the right place. Get started with PGC-1 plasmids from Addgene. References: Skeletal muscle PGC....2014.09.012. PMID: 25295784 Related Materials: PGC-1a Plasmids      ...

  34. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ...on BitesizeBio here. As part of my job ensuring plasmid quality at Addgene, I analyze 50-100 sequencing...Sequencing Resources: Tips for Using BLAST to Verify Plasmids Acknowledgements: Thank you BitesizeBio for ...

  35. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...BRDN0000561167) (Plasmid #80034) and BRAF gRNAs (BRDN0001145345 and BRDN0001146266) (Plasmid #76479 and #76480...viruses, but you can find a list of validated gRNA plasmids on our website. There are a few reasons to target...18007609. Addgene Materials Used 1. lentiCas9-Blast (Plasmid #52962) was a gift from Feng Zhang and is described... PubMed Central PMCID: PMC4486245.2. pXPR_011 (Plasmid #59702) was a gift from John Doench & David Root...

  36. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...Resources on Addgene.org Find Plasmids for FRET Browse Empty Plasmids to Fluorescent Protein Fusions ...oocytes, mainly). Nevertheless, tRNA suppression plasmids are available from depositing labs that have mastered...the potential rewards could be substantial: Plasmid tRNA Synthetase Expression Host pMAH-POLY Polyspecific...Fig. 1) which can be carried out using Addgene plasmids. His-tag labeling reagents You may already have...

  37. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ... tag plasmids here! Where should I place the tag and how many do I need? When designing a plasmid containing...from Addgene More Resources on the Addgene blog Plasmids 101: Protein Tags Antibodies 101: ChIP Antibodies...

  38. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...module, which are also available as lentiviral plasmids if you want to make a stable cell line. This design...developing embryo is accessible for injection of plasmids and other materials.    Figure 3: Optogenetic...Guide or browse our collections of optogenetics plasmids – and keep an eye out for future updates to the...on Addgene.org Optogenetics Guide Optogenetics Plasmids References Aljiboury, A. A., Ingram, E., Krishnan...

  39. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...Resource Page CRISPR plasmids designed for use in bacteria Deposit Your Microbiology Plasmids with Addgene ...

  40. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...on the Addgene Blog Plasmids for endogenous gene tagging in human cells Plasmids 101: Protein Tags Fluorescent...from many pitfalls: they require construction of plasmid vectors and/or rely on cumbersome in vitro synthesis...directly purchased and there is no need to construct plasmid vectors or synthesize/purify IVT sgRNA in the lab...SapTrap Resources on Addgene.org Browse CRISPR plasmids Check out our CRISPR guide pages Find ready-to-use...
Showing: 401 - 440 of 673 results