We narrowed to 602 results for: ACE
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TypeBlog PostPublishedMay 9, 2017, 2:12 p.m....Usually Destroyed Yes, Cpf1 cleaves 5' of the protospacer Multiplexing CRISPR-Cas9 options before Cpf1...
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REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog PostPublishedFeb. 10, 2016, 3:30 p.m.... a much lower cost. Read on to find out how to replace your SDM method with REPLACR (Recombineering of... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog PostPublishedJuly 28, 2015, 2:30 p.m....last one of those gene regulatory proteins and the places in the genome where they can be found more quickly... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog PostPublishedFeb. 24, 2015, 7:50 p.m....the protein’s C terminus. With that new design in place, Konermann immediately saw an order of magnitude... -
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur
TypeBlog PostPublishedJuly 10, 2014, 7:17 p.m....to Find a Job in Biotech and Resume/CV tips - LabSpaces Blog Find a Buisness Plan Competition Good Relations... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog PostPublishedMay 22, 2014, 4:02 p.m....Editor at F1000Research. With many changes taking place in the world of science publishing, and an ever ... -
Plasmids 101: Yeast Vectors
TypeBlog PostPublishedFeb. 25, 2014, 7:11 p.m....homologous recombination to facilitate simple gene replacement/mutation. Yeast expression plasmids used in the... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog PostPublishedFeb. 18, 2014, 4:22 p.m....managing to get together. If you all work at the same place, a 1-2 hour lunch on a regular schedule might work... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog PostPublishedDec. 10, 2013, 3:13 p.m....plasmids in the kit have the rest of the protein in place including FokI endonuclease domain as well as fluorescent... -
Addgene: The First Twenty Years
TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....hottest phone on the market, and two new websites, Facebook and Gmail, were launched. And Melina Fan, a cell... -
Transferable Skills Guide: Public Speaking
TypeBlog PostPublishedApril 20, 2023, 4 p.m....incredibly calm: “Excuse me, I seem to have lost my place. I need a minute.” I then pulled out my notebook... -
The time and cost required to make a plasmid
TypeBlog PostPublishedMarch 23, 2023, 1:15 p.m....resources, and, on the rare occasion things go wrong, replace them for free. Plasmids ship out 2-3 days after... -
All in a Twist: dsRNA
TypeBlog PostPublishedFeb. 2, 2023, 2:15 p.m.... RNA Mammalian cells have multiple receptors in place to sense dsRNA and respond accordingly. While not... -
Antibodies 101: Selecting the Right Antibody
TypeBlog PostPublishedMarch 15, 2022, 1:15 p.m....used the antibody before you will not only have peace of mind as you make your purchase but also someone... -
Antibodies 101: Secondary Antibodies
TypeBlog PostPublishedDec. 21, 2021, 2:15 p.m....indirect antibody protocols are designed to take place over multiple days. Increased run time is considered... -
What's New in CRISPR - November 2021
TypeBlog PostPublishedNov. 18, 2021, 2:15 p.m....Biotechnology Find the PRIME-del plasmids Deletion and replacement of long genomic sequences The PEDAR strategy ... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog PostPublishedAug. 5, 2021, 12:15 p.m....Aggarwal and colleagues are working on developing new surface display vectors to improve expression and trafficking... -
Visualizing Protein Turnover In Situ
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m....the rolling circle amplification could only take place if the two antibodies recognized an endogenous protein... -
CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....ortholog, and the target sequence must be located adjacent to that PAM. To scale up fluorescent CRISPR labeling... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog PostPublishedJan. 11, 2017, 3:30 p.m....getting a raise. Meanwhile, in less densely populated places like the University of Kansas and the University... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog PostPublishedDec. 8, 2016, 3:30 p.m....contains ccdB. A successful insertion will completely replace ccdB with the investigator’s insert of interest... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog PostPublishedJan. 31, 2019, 1:32 p.m....2009). Then, scientists began focusing on the extracellular matrix and fibroblasts. With each cell mechanism... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog PostPublishedJuly 21, 2016, 2:30 p.m....effects on the sexes come from, one clue or potential place to start research is by considering sex disparities... -
Addgene's Tips for Plasmid Quality Control
TypeBlog PostPublishedJan. 14, 2016, 3:30 p.m....when designing a sequencing primer, you need some space between your primer binding site and the first feature... -
Plasmids 101: Golden Gate Cloning
TypeBlog PostPublishedAug. 27, 2015, 2:30 p.m.... The destination vector and entry vector(s) are placed in a single tube containing the Type IIS enzyme... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog PostPublishedMay 5, 2015, 3:54 p.m.... – F1000 just announced the launch of their F1000Workspace. Give your favorite searching, reading and... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog PostPublishedJan. 29, 2015, 1:25 p.m....articles. Look for them on Twitter, LinkedIn and Facebook to see their online personas. Some have online... -
5 Steps to Writing Grant Proposals for Grad Students
TypeBlog PostPublishedFeb. 11, 2014, 12:13 p.m....research plan Most grant applications restrict the space available to describe the proposed work. Thus, every... -
CRISPR in the Clinic
TypeBlog PostPublishedNov. 2, 2023, 1:15 p.m....for these diseases was sponsored by Vertex Pharmaceuticals and CRISPR Therapeutics. Data shared from these... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog PostPublishedDec. 14, 2021, 2:15 p.m.... godsends. Tethering dCas9 to the histone acetyltransferase p300 or the DNA methyltransferase DNMT3A allows... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog PostPublishedJuly 23, 2020, 1:20 p.m.... viruses. For example, an Acr gene was used to replace genes in the difficult-to-engineer Sulfolobus islandicus... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog PostPublishedJuly 18, 2019, 12:55 p.m....reminded of the extent that C. elegans researchers embrace open science and share resources and tools. That... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog PostPublishedJuly 11, 2019, 1:26 p.m....create knockout mice include a reporter gene, which replaces the targeted gene and provides a way to track ... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog PostPublishedSept. 27, 2017, 1:23 p.m....opportunity is the AAAS Mass Media Fellowship, which places science students for 10 busy weeks at media outlets... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m....Miyawaki et al., 1997), was designed to measure intracellular calcium and published in 1997. Since that time... -
Adenoviral Vector Production and Troubleshooting
TypeBlog PostPublishedJune 14, 2018, 11:44 a.m....chloride (CsCl) density gradients combined with ultracentrifugation to separate rAdV from other cellular debris... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog PostPublishedOct. 6, 2020, 1:15 p.m....method, dCas9 is fused to CBP, an histone acetyltransferase domain capable of rearranging chromatin structure... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog PostPublishedAug. 9, 2022, 1:15 p.m.... contained all the goldilocks antibodies in one place... well, lucky for you one is in the making by Dr... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 3, 2022, 1:15 p.m....fragment An enhanced recombinant amino-terminal acetylation system and novel in vivo high-throughput screen... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog PostPublishedAug. 30, 2016, 2:30 p.m....this spread until vaccine programs can be put in place to provide community protection. Toehold switches... -
Plasmids 101: FLEx Vectors
TypeBlog PostPublishedApril 28, 2016, 2:30 p.m....gene “GeneR”). The key to this experiment is the placement of the two pairs of target sites. By flanking ... -
Tips for Titering Your Lentiviral Preps
TypeBlog PostPublishedMarch 15, 2016, 2:30 p.m....or single cell sorting based on expression of a surface marker, subcloning, expansion, and confirmation... -
7 Simple Strategies to Resolve Conflicts with Difficult Supervisors
TypeBlog PostPublishedJan. 21, 2015, 1:30 p.m....both personal and professional conflicts. At the workplace, these strategies will help you to communicate... -
The AAVantages of AAV in CRISPR Screens
TypeBlog PostPublishedFeb. 4, 2025, 2:15 p.m....that when using Cas9, you’re already almost out of space! Don’t let this get you down for too long, though... -
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m.... is added in the form of auxin hormone indole-3-acetic acid (IAA), the TIR1 F-box associated E3 ligase... -
Of Myc and Men
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m.... one of several new tools for unraveling the multifaceted role Myc plays in human health and disease. ... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog PostPublishedFeb. 7, 2019, 2:16 p.m....guide sequence to every possible 23bp sequence (protospacer + PAM) in the target region after HDR. If the... -
Academic vs. Industry Postdocs
TypeBlog PostPublishedNov. 13, 2018, 1:22 p.m....better understanding of the fast, deadline-driven pace of biotech. In other words, industry postdocs already... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog PostPublishedOct. 30, 2018, 1:04 p.m....In many tissues, stem cells serve to replenish/replace damaged cells that no longer function adequately... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....community if you've had success with it. Some great places to get your results out there include: Google ...