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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 610 results for: CaS;

Showing: 501 - 550 of 610 results

  3. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ... Genetic screens in human cells using the CRISPR/Cas9 system. Science, 343(6166): 80-84 (2014). doi: https...

  4. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...primer for beginners Adenoviral delivery of CRISPR/Cas9 Tips for a first time AAV user Resources on Addgene.org...

  10. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...large enough for many applications, but in some cases multiple AAVs (co-infection), an alternative virus...

  12. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ... EGFP and mEGFP. Many of these features, such as Cas9, weren’t detected by our in-house software. In fact...

  13. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ... endogenous protein fusions created using CRISPR/Cas9. eNLs offer high luminescent signal for imaging ...

  18. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...open innovation in plant synthetic biology. In our case, we selected a project in a field with 30 years ...

  20. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...markers. The MX series of antibiotic resistance cassettes is most commonly employed, with the KanMX being...

  23. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...isolating DNA without a kit. However, that isn’t the case for RNA extraction and purification. There is one...

  24. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...scientific ecosystem, enriched by open access. Lancaster et al. (2018). Of course, preprints don’t ...

  27. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...translation in living single cells. However, before CRISPR-Cas9 these techniques were quite tedious and, even here...

  29. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...the RBS, but binding of the “trigger RNA” (in this case, biomarker RNA or 16S rRNA isolated from the microbial...

  35. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...either side (often called a “lox-stop-lox” or “LSL” cassette) upstream of a gene of interest will prevent gene...

  36. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...Activation of membrane receptors and signaling cascades conceivably allows the genome to sense the impact...

  40. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...starter cells (infected with G-deleted RABV). In this case, G-deleted RABV will infect the starter cell. RABV...

  43. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...mechanisms before you start an experiment, just in case something does happen.   Finally, the best way to...

  44. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...up when your applications get rejected on some occasions. Strategize, choose calls that suit your career...

  48. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...more accurate proviral integration assay. In either case, properly titering your viral prep is the critical...
Showing: 501 - 550 of 610 results