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  1. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...collaboration. This is why she is excited to be working at Addgene, because it brings scientists together...others. This love of interacting with people and talking about science makes her the perfect Addgenie and...

  2. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...technology has really changed the game for anyone looking to quickly and easily manipulate specific genes...These pooled libraries include enrichment against kinases, cell cycle proteins, and more.  “In the past, ...

  5. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...not be expressed in the tissue/cell line you are working with. Development-specific genes – if a gene...depends on your experiment and the organism you are working in. The spacing of genes and whether or not it’...

  6. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...her talk about Addgene’s internship opportunity taking place next summer.  I applied to Addgene and began...week at Addgene. This report shared my work by breaking down the process of each day, my background, observations...

  7. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...selectivity for K+ over Na+ and rapid photocurrent kinetics. They demonstrate the use of HcKCR1 as an optogenetic...photocurrents, and is more selective for K+ than HcKCR2, making it more suitable for in vivo optogenetic use.   ...

  8. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ... to improve our resources for this community. Networking with microbiologists Over the course of the conference... technology. Addgene got several shout-outs for making popular screening libraries like Brie and Brunello...

  9. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ... be. Managers must ensure that their teams are working well together and collaborating effectively with...ability to assess and initiate things independently. Taking initiative doesn’t mean going rogue, pointing out...

  10. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ...some people find this hard so get used to it by making it a concrete goal to say “thank you, job well ... they are at risk of being fired and should be looking for a new position. One of the most common mistakes...

  12. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...properties (for example, overall brightness or on/off kinetics) that makes them suitable for different applications...with a CAG promoter exhibits high specificity for Purkinje neurons. 105321  AAV1  pGP-AAV-syn-jGCaMP7c...

  13. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...sugar may be a more attractive choice for labs looking to improve their AAV yields. Given the wide range...Clathrin-mediated endocytosis and subsequent endo-lysosomal trafficking of adeno-associated virus/phage." Journal of ...

  14. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ... days or even weeks. Primer design was done painstakingly by hand. Records of plasmid construction were...newly sequenced bacterial or viral strains. We are working on a way for SnapGene users to apply features from...

  16. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ... much-needed community resource. Instead of painstakingly searching for experimental details in research...currently still in beta version, so we’re always looking for ways to improve the viewing or data deposition...

  17. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...catalog, so that you will know exactly what you are working with. We will also be collecting feedback from ...Resource Identifiers (RRIDs) for each antibody, making it easy for you to determine whether any given ...

  18. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ... be green, containing the desired mutation but lacking both RUBY and the construct that rescues the mutation...RUBY was integrated into a CRISPR system, then tracking the segregation of the CRISPR construct could ...

  20. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...application. Depending on the tag, directly imaging or tracking your protein may also be possible, without the...also be removed by treating the protein with enterokinase, which recognizes the 5 amino acids on the C...

  21. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...You are a scientist looking to determine how Protein A and Protein B interact. You read extensive research...Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R., King, D. J., Norris, S. H., & Scher, D. S. (1994). Isotype...

  22. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ... four. No Y chromosome is present in this line, making it genetically female. HEKs are generally cultured... line for you. Are HEKs for you?   If you are looking for a human cell line model, HEKs might be a great...

  23. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...by outright stating the big-picture problem, by asking your audience a simple question, like “Have you...ending of the story.  At this point you may be thinking  “I don’t want to bury my results all the way ...

  24. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...Incredibly imaginative, funny, bittersweet, and shocking at times."     - Jason Snair, Product Designer...on today! Addgene Book Club (ABC) founder, Eric Perkins, is Addgene’s Director of Science Product Management...

  25. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...contrast” variant provides improved detection for tracking large populations of neurons in wide-field imaging...fusion fluorescent proteins can affect DREADD trafficking and localization. The Sternson lab recently deposited...

  28. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...and how it can be used in a lab? Here we’ll be talking about the ins and outs of this classification system...negative (red/pink) staining. Image credit: Mark Perkins https://www.flickr.com/photos/occbio/7092385559...

  29. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...effectiveness of CRISPR/Cas9, scientists are still looking for ways to diminish the possibility of those off-target...s Disease.” Journal of Alzheimer's disease & Parkinsonism. https://doi.org/10.4172/2161-0460.1000439 6...

  30. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    .... There’s also tips for setting writing goals, tracking progress, and starting a writing group. Loop Habit...literally a tomato! Image from Gerlos.   Tools for checking grammar and jargon Hemingway Editor  This is a...

  31. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...proteins and mutations in the trs site reduce Rep nicking. Since Rep proteins are important for viral genome...PMID: 28967288. Shitik, E. M., Shalik, I. K., & Yudkin, D. V. (2023). AAV- based vector improvements unrelated...

  32. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...you have one) to communicate your plan as well. Making logistical and funding arrangements Visiting scientist...really good time! If it sounds right for you, start thinking outside of your current research box, send out...

  34. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...centralized tracking when biological reagents are shared — both at what time and with whom. Tracking these ...

  35. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...differentiated into dentritic cells or macrophages through cytokine induction (Groell et.al, 2018). Thus, microscopic...mimic the human lymphatic system or even the human skin and subcutaneous models (Groell et.al, 2018). Treating...

  37. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...diseases in which sequence replacement holds some kind of fitness advantage but error-prone repair of the... the gene would be disastrous (i.e. if breaking the gene is worse than leaving the mutation alone). CRISPR...

  38. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...Photons Chemical reaction High-energy photons Kinetics of Photon Generation Slower Faster Cofactors...been identified in various species with differing kinetics, substrate requirements and photon emission wavelengths...

  41. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...performed to amplify the target using PCR. Instead of tracking the reaction progress as in real time PCR, each...introduced how digital PCR works, let’s explain what kind of assays can be used to sniff out your desired ...

  42. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ... and careful Addgenies, but also a lot of good tracking. Our Software and IT Teams made major upgrades...plasmids in the repository. The Outreach Team has been making sure scientists know about the new initiative and...

  44. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...information on this system! GPCR-APEX: Interested in tracking the downstream signaling pathways mediated by ... JA, Gygi SP, Kruse AC (2017) Multidimensional Tracking of GPCR Signaling via Peroxidase-Catalyzed Proximity...

  45. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...FRET-based sensors such as Twitch. While calcium is king in the sensor world, there are sensors available...expression and downstream monitoring. Nervous about making a virus for your biosensor? Don’t be! We have you...

  46. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...replicate and spread. Instead, viruses replicate by hijacking the host’s system to produce more virus and continue...viral applications to help you start (safely!) thinking about the possibilities. Ready to plan your next...

  47. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ... those first. Depending on the markers you are looking at and the antibodies you are using, you may need... general consensus seems to be that if you are looking for a rare population or are unsure what amount...

  48. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...Addgene was built on the concept of accessibility: making plasmids more accessible to scientists around the...helpfully. You and your readers are now a team, working together to share information. Sometimes it is ...

  49. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...useless without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource displays...they're available through Addgene. So whether you're looking for the exact Cas9 plasmid used by Jinek et al....
Showing: 501 - 550 of 779 results