We narrowed to 612 results for: SCR

1. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

   Type

   Blog Post

   Published

   Sept. 26, 2024, 1:15 p.m.

   ... — for example, the stem-loop structure of transcriptional terminators. Another potential issue is sequence...

2. Improved Plasmid Maps Powered by SnapGene

   Type

   Blog Post

   Published

   June 29, 2017, 1:06 p.m.

   ...the gene, the name of its gene product, and a description of its function. You can also click one location...

3. A Practical Approach to Choosing the B(right)est Fluorescent Protein

   Type

   Blog Post

   Published

   June 1, 2017, 2:30 p.m.

   ...the fluorescent protein properties need to be scrutinized under relevant conditions. The properties of ...

4. CasPEDIA: A Functional Classification of Cas Enzymes

   Type

   Blog Post

   Published

   Jan. 25, 2024, 2:15 p.m.

   ...relevant resources The homepage displays a graphic describing CasIDs and relevant properties of Cas enzymes...

5. Viral Vectors 101: Inverted Terminal Repeats

   Type

   Blog Post

   Published

   Oct. 19, 2023, 1:15 p.m.

   ...region of an ITR with a sequence containing transcription factor binding sites can increase transgene ...

6. Viral Vectors 101: Biosensors

   Type

   Blog Post

   Published

   July 6, 2023, 1 p.m.

   .... Physiological factors Unlike the previously described sensors, this class measures either factors as...

7. GCE4All: Making Genetic Code Expansion Accessible

   Type

   Blog Post

   Published

   June 15, 2023, 1:15 p.m.

   ...through a seminal paper from Peter Schultz’s lab describing a technique that allowed for intentional incorporation...

8. Antibodies 101: Flow Cytometry Controls

   Type

   Blog Post

   Published

   June 6, 2024, 1:50 p.m.

   ...controls like FMO or isotype controls, which we’ll describe a bit later. Single Colors Single color controls...

9. Sharing is Caring: But How to Distribute Open Hardware?

   Type

   Blog Post

   Published

   Feb. 28, 2023, 2:15 p.m.

   ...Researchers around the world don't have to start from scratch every time to pursue their scientific curiosity...

10. 7 Tips to Secure a STEAM Internship This Summer

    Type

    Blog Post

    Published

    Feb. 2, 2021, 2:15 p.m.

    ...consider the advice below:  1. Eligibility check Scrutinize every opportunity prior to starting an application...

11. A Deep Dive into BioBuilder

    Type

    Blog Post

    Published

    Oct. 11, 2017, 1:44 p.m.

    ...Murray’s lab uses cellular extracts to perform transcription and translation in vitro, and the new BioBuilder...

12. Plasmids 101: Yeast Vectors

    Type

    Blog Post

    Published

    Feb. 25, 2014, 7:11 p.m.

    ...differ from the bacterial elements previously described. Why do scientists use yeast vectors? Yeast are...

13. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type

    Blog Post

    Published

    Sept. 8, 2020, 2 p.m.

    ...RESOLUTE plasmid collection to perform a functional screen in mammalian cells to identify any solute channels...

14. Giving gRNAs a Facelift - Synthetic and Beyond

    Type

    Blog Post

    Published

    Oct. 20, 2022, 1:15 p.m.

    ...conformationally restricted RNA which improves mismatch discrimination and is more nuclease-resistant, resulting in...

15. Antibodies 101: Selecting the Right Antibody

    Type

    Blog Post

    Published

    March 15, 2022, 1:15 p.m.

    ...-independent assays such as mRNA data from transcriptomics; independent antibody strategies that compare...

16. Antibodies 101: Secondary Antibodies

    Type

    Blog Post

    Published

    Dec. 21, 2021, 2:15 p.m.

    ...secondary will bind to all the primary antibodies indiscriminately, giving them the same output signal. In these...

17. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    Published

    March 19, 2019, 1:31 p.m.

    ...toolbox, Ed Boyden’s lab developed a way to rapidly screen thousands of proteins for one that can report electrical...

18. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type

    Blog Post

    Published

    Jan. 31, 2019, 1:32 p.m.

    ...enzyme that controls apoptosis and regulates transcription. Without TKA1, cells proliferate and cannot ...

19. Antibodies 101: Fab Fragments

    Type

    Blog Post

    Published

    Sept. 5, 2024, 1:15 p.m.

    ...talking about here? Many of the Fab fragments described above can be used for similar applications and...

20. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type

    Blog Post

    Published

    Feb. 2, 2018, 3:20 p.m.

    ...Twitter @sejkeown. If you enjoyed this series, subscribe to the Career Channel of the blog so you don’t...

21. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...Amsterdam. The previous two posts in this series described a practical approach to selecting a bright fluorescent...

22. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type

    Blog Post

    Published

    Dec. 8, 2016, 3:30 p.m.

    ...more efficient, as one does not have to thoroughly screen colonies for the insert. Gateway® technology (developed...

23. Viral Vectors 101: An Introduction to AAV

    Type

    Blog Post

    Published

    May 30, 2023, 1:15 p.m.

    ... this DNA structure can readily fold into transcriptionally active double-stranded form through intra-...

24. Transferable Skills Guide: Public Speaking

    Type

    Blog Post

    Published

    April 20, 2023, 4 p.m.

    ...your slide deck gets uploaded. Your mic emits screeching feedback. You’re muted on Zoom. Your video conferencing...

25. The time and cost required to make a plasmid

    Type

    Blog Post

    Published

    March 23, 2023, 1:15 p.m.

    ...plasmids, using both mass production genome wide screens and the more traditional “one at a time” approach...

26. Antibodies 101: Conjugation

    Type

    Blog Post

    Published

    March 26, 2024, 1:15 p.m.

    ...Stephenson W, et al (2017) Simultaneous epitope and transcriptome measurement in single cells. Nat Methods 14:...

27. Transferable Skills Guide: Cross-team Communication

    Type

    Blog Post

    Published

    Nov. 21, 2019, 1:53 p.m.

    ...when applicable Any other relevant information Prescribing an official name to all of our plates, tubes,...

28. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type

    Blog Post

    Published

    Oct. 29, 2019, 2:52 p.m.

    ..., they are not always easy to track at the transcriptional or translational level. However, several genetic...

29. Human Germline Editing Using CRISPR

    Type

    Blog Post

    Published

    Aug. 10, 2017, 2:19 p.m.

    ...disorders, pre-implantation genetic diagnosis (PGD) screens embryos to find those that do not carry a given...

30. Hot Plasmids - October 2020

    Type

    Blog Post

    Published

    Oct. 13, 2020, 1:15 p.m.

    ...Addgene The Cas Hybrid for Multiplexed Editing and screening Applications (CHyMErA) Knockout Libraries express...

31. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type

    Blog Post

    Published

    June 25, 2020, 1:15 p.m.

    ... I can have on an organization beyond my job description.” Our resident bagel provider, source of energy...

32. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    Published

    Aug. 24, 2021, 1:15 p.m.

    ...ELISA is a modification of the sandwich ELISA as described here, the direct and indirect ELISA (more details...

33. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type

    Blog Post

    Published

    May 4, 2021, 1:15 p.m.

    ...to share or remix the songs, the CDs could get scratched over time, and it was difficult to keep track ...

34. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    Published

    June 26, 2018, 1:33 p.m.

    ...proteins in the lab, clinicians might consider prescribing GdnHCl to patients suffering from rare diseases...

35. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    Published

    June 14, 2018, 11:44 a.m.

    ...20 additional days at which time the cells are scraped, and lysed by multiple freeze-thaw cycles. The ...

36. Rabies and Neuronal Tracing

    Type

    Blog Post

    Published

    May 29, 2018, 1:51 p.m.

    ...widely used in neuroscience. In this post, I’ll describe how rabies virus (RABV) can be used in the brain...

37. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type

    Blog Post

    Published

    May 22, 2018, 1:38 p.m.

    ...corresponding S. cerevisiae met deficiencies (manuscript in preparation), indicating that Met protein ...

38. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    Published

    March 7, 2024, 2:39 p.m.

    ...Between SpRY and several of the other Cas variants described, nearly all genomic landscapes should be editable...

39. CRISPR in the Clinic

    Type

    Blog Post

    Published

    Nov. 2, 2023, 1:15 p.m.

    ...potential for treatment is no longer an accurate descriptor; progress in clinical therapy is more fitting...

40. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    Published

    Aug. 10, 2023, 1 p.m.

    ...populations of interest. Many of the calcium sensors described are also already packaged into viral vectors and...

41. High Complexity Golden Gate Assembly with NEB’s DAD

    Type

    Blog Post

    Published

    Aug. 1, 2023, 1:15 p.m.

    ...Additionally, this assembly, which used only antibiotic screening for selection, failed under normal cycling protocols...

42. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    Published

    June 22, 2023, 1:15 p.m.

    ...involve needles or scalpels (sharps). Cuts and scrapes from sharp equipment are one of the most common...

43. 6 Tips for Grant Writing

    Type

    Blog Post

    Published

    Oct. 18, 2016, 2:30 p.m.

    ...are unlikely to get past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor...

44. Tips for Technical Support Calls

    Type

    Blog Post

    Published

    Sept. 20, 2016, 2:39 p.m.

    ...plasmids and pooled libraries were originally described in a peer-reviewed scientific publication. We ...

45. Overcoming the Challenges of Lentiviral Production

    Type

    Blog Post

    Published

    Aug. 7, 2018, 12:31 p.m.

    ...systems, this system incorporates the Tet-off transcriptional activation system to drive high levels of expression...

46. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type

    Blog Post

    Published

    Oct. 22, 2019, 12:20 p.m.

    ...poster format differs in many ways from writing a manuscript. If you have ever attended a scientific conference...

47. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    Published

    Sept. 26, 2019, 12:50 p.m.

    ... can also deliver Cas9 variants, such as a transcriptional activator dCas9-VPR, if these variants are ...

48. Science communication: 9 Strategies to Get Your Foot in the Door

    Type

    Blog Post

    Published

    Sept. 27, 2017, 1:23 p.m.

    ...now. These 9 strategies will get you started:  Subscribe to Career Blog Posts from Addgene 1) Twitter –...

49. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    Published

    July 14, 2016, 2:30 p.m.

    ...for Cas9 (42 nt vs 100 nt.) However, the newly described non-CRISPR protein NgAgo is smaller than any of...

50. Experimenting in an Open Source lab: from CRISPR to Cats

    Type

    Blog Post

    Published

    July 23, 2015, 2:30 p.m.

    ...manual skills at the regular microscope, and the scripting skills at the automated microscope  to keep our...