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  1. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...reflected light by using a pinhole.  However, the excitation light excites all of the fluorophores as it passes...takes to image through a large z-stack (8).  One limitation of LSFMs using single side illumination, especially...

  2. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...BLAST (blastx) search to check for expected ORFs, mutations or truncations. A primary advantage of blastx ...database. Another benefit is that a frameshift mutation present in the ORF is readily apparent when viewing...

  3. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...the central dogma. We have the beginnings of computational logic and data storage. What we don't have are...begin to imagine designing 10-gene systems with quantitative precision. There are few designers of biological...

  5. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...the world is unprepared. Each year in the United States, two million people will be infected by antibiotic...defective phagemid; Bikard et al. did not observe any mutations at the target site. In a mouse skin colonization...

  6. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...were more prohibitive at the time. With these limitations no longer an issue, bacterial in vivo cloning... N-terminus of a protein-coding sequence, or a mutation can be introduced in a gene, while adding a GFP-tag...

  7. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses, 13(7), 1288. https://... the National Academy of Sciences of the United States of America, 54(1), 137–144. https://doi.org/10.1073...

  8. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...Fixation allows you to freeze your samples in a given state or point in time. You want to halt degradative processes...methanol, or ethanol, work by dehydrating and precipitating cellular components. This method of fixation...

  9. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...studies (e.g. using mariner), as a method of tagmentation for next-gen sequencing pipelines (e.g. using...the mini-transposon can occur in two different orientations, but is strongly biased towards T-RL, where ...

  10. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...storage may be a bit dubious due to lack of documentation and high turn-over of lab personnel. If you ...These binding events can be impaired by the electrostatic repulsion of the negatively charged cell with...

  11. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...structures in 10 closely related yeast strains. Adaptation to changing conditions - Organisms often need...sometimes (but not always) be noted in in the “mutation” field on the plasmid page (see plasmid 87904 ...

  13. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...definitely glad we did - you can see some of the stats from the AMA to the right - the Reddit community...r/Science has a great team of moderators who facilitated scheduling, filtered out inflammatory questions...

  14. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...floating on the water surface. The latter reflects a state of behavioral despair, and the more time the animal...showed that the activity of a particular subset of excitatory neurons (Drd1) in mPFC is responsible for the...

  15. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...cleaves a substrate, which will then form a blue precipitate. GUS is usually the first choice for establishing...transformation success. Luciferases are great quantitative reporters and use a substrate to produce bioluminescence...

  16. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin-1 (pan-neuronal) promoters...cell types (microglia are a notable exception) necessitates such molecular gymnastics for sensitive applications...

  17. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...amount of marker that is present, which would be annotated like so: CD14high or CD14low.  Don’t be intimated...liver and kidney recipients. World Journal of Transplantation, 8, 23–37. https://doi.org/10.5500/wjt.v8.i1.23...

  18. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...: creativity. I’ve heard quite a few scientists state that they’re not creative, usually saying that they...your break can be as simple as a few minutes of meditation or a walk to the break room for a sip of water...

  19. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ... E. coli B strains, 1 E. coli W strain, and 1 Tatumella citrea, with a total of 70 sites edited. We optimized...strain library with different gene dosages to facilitate swift optimization of strains. Multiple induction...

  20. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...Nature report from researchers at Amgen, it was stated that only 11% of the academic research in the literature...with them including reports and protocols. To facilitate this, some ELNs allow you to invite people and...

  21. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ... in the lab are protein purification and immunoprecipitation experiments. Biotinylated proteins or complexes...non-selectively label DNA and RNA. Photobiotin acetate consists of a biotin molecule attached to a photoreactive...

  22. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...Medicago truncatula and several crop species including potato, corn, tomato, wheat, mushroom, and rice (Khatodia...Lew TTS, Sweeney CJ, Koman VB, Wong MH, Bohmert-Tatarev K, Snell KD, Seo JS, Chua N-H, Strano MS (2019)...

  23. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...the architecture of my cells,” or “How does this mutation impact the types of cells found in my tissue.”...are using secondaries with fluorophores whose excitation/emission spectra do not overlap. Multiplexed ...

  24. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...zoom on a camera lens without having to physically rotate the rings around the lens. It allowed me to focus... 4).  Learn: This works great for on-camera presentations and interviews. But how can I adapt this setup...

  25. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ... signal from low abundance targets. Analysis/Presentation Once you’ve captured your images, various software...each cell. When making figures for a paper or presentation, make sure the images are accessible to all ...

  26. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...the transformed bacterial culture into the late stationary phase. Growing the culture at a lower temperature...low metabolic burden seed-stock and fed-batch fermentation processes. Biotechnol Bioeng. 2009 Aug 15;103...

  27. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...gene is also useful in case the gene has a weird mutation that you didn’t know about (which could affect...theory you could use densitometric analysis to quantitate the intensity of the bands on your gel and get...

  29. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...resembles cas10. All type IV subtypes are missing adaptation genes, Cas1 and Cas2, which are critical for ... become the most engineered Cas enzyme, with adaptations for increased fidelity, activation/repression...

  30. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...cells used and the readout, this could be a major limitation. An alternative to the full genome CRISPR screen...PhD student. In particular, one slide of the presentation made a lasting impression on me. The slide showed...

  31. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...being able to specify and correct single-base mutations without introducing DSBs at all. Base editing ...are smaller and less likely to cause off-target mutations than those derived from naturally occurring cytidine...

  32. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...lane 1 is‌ lane 8. Common ways to keep membrane orientation straight are to mark the upper left-hand corner... Ensuring wash is happening under sufficient agitation (rocking) Add Tween-20 to wash buffer (can...

  33. New Antibodies in the Lab Animation!

    Type
    Blog Post
    Published
    Nov. 19, 2024, 2:15 p.m.
    ...study proteins via ELISAs, western blots, immunoprecipitation, immunohistochemistry, immunocytochemistry...

  34. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    Published
    Sept. 5, 2023, 1:15 p.m.
    ...they go to Addgene.”-Ryan Mehl, Professor, Oregon State University. ...

  35. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    Published
    Sept. 20, 2022, 1:15 p.m.
    ...be invited to compete in our speed round data presentation, with two winner each receiving a $500 travel...

  36. 2022 Depositor Week

    Type
    Blog Post
    Published
    Dec. 12, 2022, 2:15 p.m.
    ...  Monday: Thank you post (this is it!)  Tuesday: Stats roundup on Twitter Wednesday: 2022 Blue Flame Depositors...

  38. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...Addgene shipping and ordering processes, don’t hesitate to contact our friendly customer support team ...

  41. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ... new tool for making diverse, localized point mutations. Listen to Our Segment on CRISPR X Hot Plasmid...

  42. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...cells are screened for phenotypes of interest. Limitations of CRISPR Precise edits are difficult. CRISPR...

  44. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ... convenience. After an order is placed, we’ll facilitate the necessary MTA, then start production and ...

  45. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...for Gene Expression in Mammals Recombinase-based State Machines Enable Order-dependent Logic in vivo As...

  48. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    Published
    Jan. 15, 2019, 2:19 p.m.
    ... year, @BordeauxFoSho takes you through our interpretation of Buddy the Elf's journey from the North Pole...

  49. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ...standards (* above) will be given a DOI for easy citation by others and, when published, will meet FAIR ...
Showing: 501 - 550 of 704 results