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Showing: 201 - 220 of 545 results

  2. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ... In frogs, ectopic expression of ion channels, despite having an otherwise normal genomic background, ...Subsequent cutting results in a two-headed worm, despite the fact that it’s genetically wild type  (Oveido...

  4. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...a slash, that indicate the ITR and serotype, respectively. For example, AAV2/8 has a type 2 ITR and a ...serotype selection. Promoters and serotype control, respectively, cell-specific gene expression and viral infection...

  5. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...was released. This blog has not been updated in response to this paper. Any hint of CRISPR editing in human...still be helpful in increasing viable embryos, especially in the case of older mothers, for whom egg retrieval...

  6. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...boxB Magenta boxB-MS2 White boxB-MS2-PP7 Despite the success of Ma et al.’s approach, fluorescently...fluorescent proteins BFP, GFP, and RFP fused to the respective hairpin binding domain. Pairs of identical hairpin...

  7. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...www.addgene.org/guides/crispr/” — SirHamhands, responding to a request for “any suggestion for CRISPR online...2023 conference. In 2020, the world changed in response to the COVID-19 pandemic, and Addgene pivoted ...

  8. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...request to any of these people, including and especially the hiring manager. This is considered intrusive...own good ideas, events and favorite resources. Respect the network and it will reward you. Resources ...

  10. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...abundance of a particular barcode sequence then corresponds to the abundance of a specific clone; lineage... C. Bhang, et al. "Studying clonal dynamics in response to cancer therapy using high-complexity barcoding...

  12. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...successful outcome and the good ones take this responsibility seriously. As I make my rounds speaking at ...Schachter Consulting is part of that group and she is especially excellent in coaching for communication skills...

  16. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ..., some adenovirus strains are known to cause respiratory diseases in humans, earning this virus a BSL-... and closely follow safety protocols. This is especially true when you’re tired, in a rush, or having ...

  17. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...great tips to guide your experimental design, especially the design of the RNA sequences you’ll use in...increase the odds of incorrect editing outcomes, especially when MMR is inhibited. Created with BioRender.com...

  18. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ... from the membrane along with the antibodies, especially with more stringent stripping methods. If you...horseradish peroxidase (HRP) or Alexa Fluor dyes, respectively. Chromogenic detection produces colored precipitates...

  20. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...experience helping scientists share plasmids, especially those of the CRISPR variety (see slides from ...conserved in mammals, expressed in neurons, and are especially abundant in neuronal synapses. shRNA silencing...
Showing: 201 - 220 of 545 results