We narrowed to 316 results for: Mut

1. New Norepinephrine Indicators: nLightG and nLightR

   Type

   Blog Post

   Published

   Dec. 12, 2023, 2:15 p.m.

   ...adrenergic receptor; nLightG contains circularly permuted green fluorescent protein (GFP), and nLightR uses...

2. 6 Steps to Submitting a Resume That Gets Seen

   Type

   Blog Post

   Published

   July 9, 2015, 2:30 p.m.

   ...manager on paper or as an email send directly from a mutual connection. If the organization is small, any person...

3. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

   Type

   Blog Post

   Published

   Feb. 1, 2018, 3:07 p.m.

   ...confirm the identity of the gene and check for any mutations. Once we identify the gene, we can easily annotate...

4. Build Your CRISPR Vocabulary

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   Blog Post

   Published

   July 2, 2024, 1:15 p.m.

   ...other hand, viruses can evolve to evade CRISPR by mutating these sequences. Pro tip! Various labs have created...

5. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

   Type

   Blog Post

   Published

   June 11, 2020, 1:34 p.m.

   ... The opsin ChETA, for example, is a ChR2 opsin mutated to have faster off kinetics and allow neurons to...

6. Hot Plasmids - October 2022

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   Blog Post

   Published

   Oct. 6, 2022, 1:15 p.m.

   ...high-throughput screening platform to apply saturating mutagenesis on 21 positions in the protein sequence of the...

7. Plasmids 101: Gene, enhancer, and promoter trapping

   Type

   Blog Post

   Published

   May 2, 2023, 1 p.m.

   ...to generate a collection of monitored genes and mutants - viral integration, transposon, and many more!...

8. Hot Plasmids and Viral Preps - July 2021

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   Blog Post

   Published

   July 27, 2021, 1:15 p.m.

   ...control and a SL-sensitive fluorophore (circularly permuted green fluorescent protein or cpGFP) to the petunia...

9. Sleeping Beauty Awakens for Genome Engineering

   Type

   Blog Post

   Published

   June 30, 2015, 2 p.m.

   ... exciting tool for producing specific genetic mutations and gene disruptions without the use of viral ...

10. History of CRISPR Cas - A tale of survival and evolution

    Type

    Blog Post

    Published

    Nov. 25, 2013, 10:12 p.m.

    ...CRISPR/Cas defense by generating a single point mutation in the PAM sequence, preventing the Cas nucleases...

11. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type

    Blog Post

    Published

    June 9, 2016, 2:30 p.m.

    ... Cas9 Homolog Cpf1 Check Out High Fidelty Cas9 Mutants Browse All Genome Engineering Posts Additional...

12. Plasmid Cloning by PCR

    Type

    Blog Post

    Published

    March 29, 2016, 2:30 p.m.

    ...to use a high fidelity polymerase to minimize mutations. The fidelity of the polymerase becomes more important...

13. CRISPR Challenges: Standardization and Homology Directed Repair

    Type

    Blog Post

    Published

    Feb. 14, 2018, 2:36 p.m.

    ...Kwart, including a detailed look at how cut-to-mutation distance affects editing efficiency. Optimizing...

14. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type

    Blog Post

    Published

    Jan. 25, 2018, 2:09 p.m.

    ...receptor and subsequently evaluated interactions with mutant and wild-type erythropoietin molecules. The in ...

15. 10 Great Guest Posts We're Thankful For

    Type

    Blog Post

    Published

    Nov. 18, 2016, 3 p.m.

    ...fuel sources.” In her work, Dr. Polka made R-body mutants that respond to different pH’s and some that are...

16. New Viral Vectors - Winter 2025

    Type

    Blog Post

    Published

    Jan. 28, 2025, noon

    ...viral prep with multiple serotypes pAAV-hSyn-GRAB_OTmut AAV1 Biosensor Yulong Li New viral prep pAAV-CAG-NIR-GECO2G...

17. Hot Plasmids and Viral Preps - September 2021

    Type

    Blog Post

    Published

    Sept. 21, 2021, 1:15 p.m.

    ...modify up to 60% of the disease-causing point mutations. They allow single-nucleotide modifications to...

18. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type

    Blog Post

    Published

    Sept. 14, 2021, 1:15 p.m.

    ...cloning strains, browse the table of common gene mutations in our Plasmids 101: Common Lab E. coli Strains...

19. Antibodies 101: Epitope Tags

    Type

    Blog Post

    Published

    May 11, 2023, 1:15 p.m.

    ...Tags can enable the separation of detection of a mutant protein vs. endogenous WT levels of the same protein...

20. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type

    Blog Post

    Published

    March 24, 2016, 2:30 p.m.

    ...if breaking the gene is worse than leaving the mutation alone). CRISPR delivery by nucleofection The in...