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TypeBlog PostPublishedJan. 16, 2014, 2:56 p.m....scientist-in-training will spend 10 or more years with a small number of formal advisors learning how to be a scientist...
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5 Uncommon Ways to Gain Career Skills During an Uncommon Time
TypeBlog PostPublishedOct. 20, 2020, 1:15 p.m....you are in a lab, your PI is likely working on a number of them. Find one to apply to or offer to help ... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m....with broccoli aptamers or MS2 tags using the same number of fluorophores. You can find plasmids containing... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog PostPublishedFeb. 14, 2018, 2:36 p.m....Editing, gets over 4,000 views a month. To me, these numbers show that this basic concept of gRNA design is ... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog PostPublishedJan. 25, 2018, 2:09 p.m....biotin incorporation and to a lesser extent, the number of added biotins per molecule cannot be well-controlled... -
Intro to Our Summer SciComm Series!
TypeBlog PostPublishedJune 9, 2022, 1:15 p.m....an audience? An audience can be defined by any number of characteristics. Common ones include age range... -
Antibodies 101: Epitope Tags
TypeBlog PostPublishedMay 11, 2023, 1:15 p.m....Applications of epitope tags The tags A number of epitope tags exist – all with individual optimizations... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog PostPublishedSept. 14, 2021, 1:15 p.m....your vector is large (>10 kb) or has a low copy number, try following protocols for low copy plasmids.... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog PostPublishedJune 22, 2021, 1:15 p.m....neutralization. “That degree of escape from a very small number of mutations was surprising,” says Balazs. ... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog PostPublishedMay 25, 2021, 2:29 p.m....few years for a new project to reach a sufficient number of distributions to cover costs, which can limit... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog PostPublishedAug. 18, 2023, 4:15 p.m....to dive into how they’re used! We have a growing number of Viral Vectors 101 posts about each type, so ... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m.... with a high MOI, which is beneficial for a low number of cells and in vivo settings. Find the sgRNAiBAR... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog PostPublishedMarch 5, 2020, 2:15 p.m....helpful to report the genetic background and catalog number or RRID if it exists. This is helpful because viewers... -
Fluorescent Biosensors for Measuring Autophagic Flux
TypeBlog PostPublishedJan. 22, 2019, 2:41 p.m.... for studying autophagy focused on counting the number of autophagosomes. This approach, however, has ... -
Viral Vectors 101: Transductions
TypeBlog PostPublishedApril 30, 2024, 1:15 p.m....an easier way? In fact, there is! Addgene has a number of ready-to-use viral preps available for a variety... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....transcripts of interest without an increase in the number of steps in the protocol. IgnaviCas9’s incredible... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog PostPublishedJune 9, 2016, 2:30 p.m....Addgene Cas9 and NgAgo head to head NgAgo has a number of unique features compared to Cas9. These include... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog PostPublishedOct. 29, 2015, 2:30 p.m...., and pUC were developed to provide higher copy number vectors that could be used in these cloning experiments... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog PostPublishedSept. 15, 2015, 2:30 p.m....migraines. Those attacks can be brought on by a number of stressors, including emotional stress or changes... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog PostPublishedAug. 25, 2015, 2:30 p.m.... response, I was still able to get through to a number of experts, including Harvard/MIT faculty, venture...