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...scientist-in-training will spend 10 or more years with a small number of formal advisors learning how to be a scientist...

...with broccoli aptamers or MS2 tags using the same number of fluorophores. You can find plasmids containing...

...Editing, gets over 4,000 views a month. To me, these numbers show that this basic concept of gRNA design is ...

...you are in a lab, your PI is likely working on a number of them. Find one to apply to or offer to help ...

... with a high MOI, which is beneficial for a low number of cells and in vivo settings. Find the sgRNAiBAR...

...an audience?  An audience can be defined by any number of characteristics. Common ones include age range...

...your vector is large (>10 kb) or has a low copy number, try following protocols for low copy plasmids....

...to dive into how they’re used! We have a growing number of Viral Vectors 101 posts about each type, so ...

...Applications of epitope tags   The tags A number of epitope tags exist – all with individual optimizations...

...transcripts of interest without an increase in the number of steps in the protocol. IgnaviCas9’s incredible...

... for studying autophagy focused on counting the number of autophagosomes. This approach, however, has ...

...helpful to report the genetic background and catalog number or RRID if it exists. This is helpful because viewers...

...an easier way? In fact, there is! Addgene has a number of ready-to-use viral preps available for a variety...

...neutralization. “That degree of escape from a very small number of mutations was surprising,” says Balazs.   ...

...few years for a new project to reach a sufficient number of distributions to cover costs, which can limit...

...biotin incorporation and to a lesser extent, the number of added biotins per molecule cannot be well-controlled...

...Addgene Cas9 and NgAgo head to head NgAgo has a number of unique features compared to Cas9. These include...

..., and pUC were developed to provide higher copy number vectors that could be used in these cloning experiments...

...migraines. Those attacks can be brought on by a number of stressors, including emotional stress or changes...

... response, I was still able to get through to a number of experts, including Harvard/MIT faculty, venture...