We narrowed to 289 results for: Pol

1. Celebrate National Book Month with Addgene

   Type

   Blog Post

   Published

   Oct. 3, 2019, 12:47 p.m.

   ...(which had been lost for an age) fight in the Napoleonic Wars and against something even more sinister...

2. Finding and Joining Your Dream Lab

   Type

   Blog Post

   Published

   April 8, 2014, 2:50 p.m.

   ...or contract, you should be starting to send out polite emails with your CV to people you’d like to work...

3. Viral Vectors 101: Biosensors

   Type

   Blog Post

   Published

   July 6, 2023, 1 p.m.

   ...2016 Jun 30;60(1):1-8. DOI: 10.1042/EBC20150001 Leopold, A. V., Shcherbakova D. M., Verkhusha, V. V., Fluorescent...

4. AAV Q&A with Tim Miles

   Type

   Blog Post

   Published

   May 17, 2022, 1:15 p.m.

   ... before considering the need for some manner of polyA. In this scenario, you may be best served by a dual...

5. Hot Plasmids - February 2022

   Type

   Blog Post

   Published

   Feb. 22, 2022, 2:15 p.m.

   ...individually (~20,000 lanes/50 mLs) or through two polycistronic expression vectors (3570 lanes/50 mLs) coexpressing...

6. Sweating the Small Stuff: Details in the Lab

   Type

   Blog Post

   Published

   Feb. 8, 2022, 2:15 p.m.

   ...at odd hours, used more secluded workspaces, or politely asked to not be disturbed when they were at the...

7. Antibodies 101: Buffers, Storage, and Conjugates

   Type

   Blog Post

   Published

   Feb. 1, 2022, 2:15 p.m.

   ...Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies...

8. Antibodies 101: Isotypes

   Type

   Blog Post

   Published

   Oct. 19, 2021, 1:15 p.m.

   ...lot of ways to classify antibodies: monoclonal, polyclonal, scFvs, nanobodies, and the list goes on. But...

9. 7 Tips to Secure a STEAM Internship This Summer

   Type

   Blog Post

   Published

   Feb. 2, 2021, 2:15 p.m.

   ...previously mentioned, enlist the help of your network to polish the final draft prior to submission. When in doubt...

10. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type

    Blog Post

    Published

    March 17, 2020, 1:32 p.m.

    ...III. Gene III is under the control of a T7 RNA polymerase and cannot be expressed until the base editor...

11. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type

    Blog Post

    Published

    May 11, 2017, 2:30 p.m.

    ...exciting computational algorithms, such as SIFT or PolyPhen to approach this challenge, using attributes of...

12. Screening for Successful Genome Editing with Digital PCR

    Type

    Blog Post

    Published

    March 30, 2017, 2:30 p.m.

    ...PMCID: PMC3773023. 7. Vouillot, L., Thélie, A. & Pollet, N. Comparison of T7E1 and surveyor mismatch cleavage...

13. Plasmids 101: Inducible Promoters

    Type

    Blog Post

    Published

    Jan. 18, 2018, 2:34 p.m.

    ...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...

14. Communicating Your Science With Help From ComSciCon

    Type

    Blog Post

    Published

    July 19, 2018, 1:12 p.m.

    ...broader impacts for their work in education and policy and to draw attention and support to work in their...

15. A Deep Dive into BioBuilder

    Type

    Blog Post

    Published

    Oct. 11, 2017, 1:44 p.m.

    ...whose work is focused on education and education policy. Additional Resources on the Addgene Blog Synthetic...

16. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type

    Blog Post

    Published

    Feb. 10, 2016, 3:30 p.m.

    .... Using commercially available high-fidelity polymerases such as KOD and Phusion, which are recommended...

17. Viral Vectors 101: An Introduction to AAV

    Type

    Blog Post

    Published

    May 30, 2023, 1:15 p.m.

    ...vector. Full-length ssAAV vector genome of both polarities are rescued from the vector plasmid and individually...

18. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type

    Blog Post

    Published

    Dec. 8, 2022, 2:15 p.m.

    ...to detect variants such as single nucleotide polymorphisms. Fast facts – PAM requirement: none. Best ...

19. Summer SciComm: Preprints

    Type

    Blog Post

    Published

    Aug. 16, 2022, 1:15 p.m.

    ...comment online! Want the general public (say, policymakers or community organizations) to use your work...

20. dTAG - You're it!

    Type

    Blog Post

    Published

    June 21, 2018, 2:06 p.m.

    ...proximity with the target protein, leading to polyubiquitination of the target protein and proteasomal degradation...