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We narrowed to 610 results for: REV

Showing: 201 - 220 of 610 results

  1. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...RNAs. Annual Review of Biochemistry. 2012;81(Volume 81, 2012):145-166. doi:10.1146/annurev-biochem-051410...transcription start site function would also help to reveal the impacts of a lncRNA on nearby genes and its...

  2. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...field forward to where it is today. Below we will review some of the CRISPR-based editing therapies that...’s own cells that have been modified ex vivo, previously edited healthy donor cells can be delivered immediately...safety is important for allogenic therapies to prevent a graft vs. host response. Off-target effects are...

  3. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...symposia held across the USA during this time have revealed that young scientists are aware of, and are engaged...Academy of Sciences. “The Postdoctoral Experience Revisited” 2014 Appendix B p93-95). For those advocating...25653845. Pubmed Central PMCID: PMC4304227. 4. Revitalizing biomedical research: recommendations from the...

  4. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...a table. It adds a formality and distance that prevents interaction with each other and the audience. ...moderator takes control It is the moderator's job to prevent anyone from derailing the show. The moderator must... active at all times keep things moving and to prevent any one panelist or audience member from taking...

  5. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...for dicots. NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right: T1 tomato plants that inherited...genetically-encoded voltage indicator (GEVI) than previous versions. ASAP5 can be imaged using one- or two-photon...across many cell types in varying conditions, revealing context-dependent responses and molecular patterns...

  6. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...editor (typically a Cas9 H840A nickase fused to a reverse transcriptase) together with a prime editing guide...sequence containing a primer binding site and a reverse transcription template (RTT). The design of the...and highly versatile genome manipulation. Nature Reviews Genetics, 24(3), Article 3. https://doi.org/10.1038...

  7. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ... series from Subach et al.) were derived from previously developed monomeric fluorescent proteins mKO ...dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer.... German. "Chronology of islet differentiation revealed by temporal cell labeling." Diabetes 58.8 (2009...

  8. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...Inspired Engineering. CRISPR/Cas9 technology has revolutionized the fields of molecular biology and bioengineering...which variant of Cas9. To circumvent this problem, previous studies have proposed the concurrent use of “orthogonal...gene of interest, Cas9 forms a steric block that prevents RNA polymerase from efficiently transcribing the...

  9. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...is suitable for your application. We have previously reviewed many common lab E. coli strains on the blog...optimal growth, as insufficient gas exchange will prevent your cultures from reaching the desired density...

  10. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ... to repair DNA. The DNA ends are chewed back to reveal homology, allowing the strands to anneal. DNA synthesis...Using MMEJ for gene knock-in Building on the lab’s previous work, Sakuma et al. describe a detailed protocol... using much shorter sequence homologies. Abbreviated protocol for PITCh Step 1: Generate microhomologies...

  11. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ...experience. Along with the updated search, we’ve also revamped our homepage and mascot Blugene (check back on...search terms and potential results. Just as in the previous layout, the number of plasmids matching each filter...the source organism for a gene insert. (e.g. S. cerevisiae) Plasmid Type: Choose an empty backbone if you...

  12. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...tethered to a repressive domain, such as KRAB, to prevent efficient transcription from sgRNA targets. CRISPRi...gain of function screens. These screens can help reveal the function of lowly expressed genes or genes ... sgRNAs were selected and ranked based on the previously established sgRNA rules and  potential on- and...

  13. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    Published
    Aug. 27, 2020, 1:15 p.m.
    ...inhibitors are tested in parallel for their ability to prevent BiFC resulting from the assayed PPI. This high-...However, it’s important to note that BiFC is irreversible — FP fragments become covalently bonded by associating...analysis of protein homomerization in Saccharomyces cerevisiae. Genome Res 29:135–145. https://doi.org/10.1101...

  14. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...more about how nanobodies are identified in this review. If you just want to use the nanobodies described...picking a secondary nanobody, it’s important to review a few characteristics of your primary antibody:...Nanobodies: natural single-domain antibodies.” Annual review of biochemistry 82 (2013): 775-97. PubMed PMID:...

  15. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    Published
    July 1, 2025, 1:15 p.m.
    ... for granted. In this post, we take a moment to review what exactly you are measuring and how to interpret...starting from the beginning. Before trying again, revisit your purification protocol. Are any of your materials...and quality than we have covered here! Be sure to review resources on the specific instrument you use and...

  16. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...way to improve the photon budget. Of course, “reverting” to small molecule dyes seems daunting—no one ...enzyme variant tag that reacts specifically and irreversibly with a small substrate ligand motif attached...strategies, several groups—including mine—have been revisiting the old chemistry of dyes. The first synthetic...

  17. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ... both favors antibiotic sensitive bacteria and prevents sensitive bacteria from acquiring resistance genes...settings where antibiotic resistant bacteria have previously flourished.   Figure 2: Two-phage strategies...Infection with the lytic phage kills bacteria not previously infected by the lysogenic phage, rendering the...

  18. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...hybrid systems were developed in Saccharomyces cerevisiae in 1989 and are still used extensively to screen...heterologous systems (e.g. mammalian proteins in S. cerevisiae). While this may be difficult to resolve for...Kamuzinzi, R., Ledent, V., Blaudez, D., Sanders, D., Revuelta, J.L., Boles, E., André, D., and Frommer, W.B....

  19. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...the limitations associated with SpCas9. Cas13a (previously referred to as C2c2), has several unique properties...adopts an enzymatically “active” state rather than reverting to an inactive state like Cas9 or Cpf1. As a result... Recombinase Polymerase Amplification (RPA) or Reverse-transcription RPA (RT-RPA), respectively. Amplified...

  20. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ...itself to be quite the mental puzzle if you lack previous experience with it. Don’t worry, we’ve got you...to be in the same (forward) or in the opposite (reverse) orientation? The answers to questions like these...plasmid map and identify any mismatches that are preventing your overhangs from aligning properly. Do this...
Showing: 201 - 220 of 610 results