We narrowed to 478 results for: SEC

...long-read technologies. Short-read sequencing (“Second-generation sequencing”)  Short-read sequencing ... or complex sequences such as G-C rich areas, secondary structures, or repetitive regions. Short-read ...

... date with their third party libraries and any security updates. Most of the time, updating these libraries... which means there will be no further updates, security or otherwise. This might sound like Greek to you...

...salts may be preferable if the protein product is secreted to the medium or if slow expression is required...required due to solubility concerns. Insoluble and Secreted Proteins: The most common purification protocols...

...in a single step and serve as donors for two consecutive CRISPR-mediated HDR events which results in the...target site after the first HDR event, then the second HDR event can’t occur. Lastly, depending on its...

...aeruginosa. These toxins were a mix of Type VI secreted effector toxins, toxin-antitoxin type toxins, ...Importantly, to prevent activation of toxins before the second crossover step, they tested their expression under...

... experiments, biotin is added to a primary or secondary antibody that targets your protein of interest... AviTag expressing proteins in bacteria, yeast, insect and mammalian cells. Check out Kay et al., 2009...

...Joachim Goedart, an assistant professor at the Section of Molecular Cytology and van Leeuwenhoek Centre...Joachim Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre...

...multiple plasmids. For simplicity, we’ll use the second-generation system as an example.  The lentiviral...remember: You can never trust a lentivirus with secrets... Because they tend to insert themselves into ...

... (Nilaratanakul et al., 2017) and levels of the second messenger cyclic di-AMP in Gram-positive bacteria...fluorescent biosensors for live cell imaging of second messengers cyclic di-GMP and cyclic AMP-GMP." Journal...

...usually ensure that the 3’-base(s) do not form secondary structures. The introduction (or ablation) of ...around 3% final concentration). The DMSO reduces secondary structures of the DNA template, and may also decrease...

...through cytotoxicity or the immune response. The second strategy is the simple removal of the HIV-1 genome...to ablate a well-established viral reservoir. A second challenge for CRISPR HIV-1 therapies is sequence...

... the first number refers to the genome, and the second number to the capsid. Most vectors provided by ...Issa et al., 2023). AAV2 Despite being isolated second, AAV2 is the best studied and most commonly used...

...of biting my tongue and gratefully moved on. My second rotation was in a big lab with a famous PI who ... I’ll highlight some of these questions in the sections below. Mentoring relationships can help identify...

...several applications of this technology. In each section below, we cite the original paper but link to plasmids...factors present unique challenges for study. These secreted signaling proteins regulate vital cell and tissue...

...#198003)) and Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Thermo Fisher A-21131... or Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (Thermo Fisher A-11012...

... that we describe in the Materials and Methods section of our paper (1). Lastly, it is possible to build...sequencing as described in the Material and methods section of our paper (1). How might one troubleshoot GSRMs...

...Conference highlights included remarks by the Spanish Secretary of Research, Development, and Innovation, D. Carmen...quantifiable, but always significant. Doubts, fears, insecurities… all shrink when shared with a mentor who has...

...complex samples such as whole cell lysate or tissue sections. It is important to confirm that your antibody...doi.org/10.1080/19420862.2015.1100787  Taussig MJ, Fonseca C, Trimmer JS (2018) Antibody validation: A view...

...of the techniques listed below and always use a second assay to be 100% sure. Before testing for mycoplasma...time-consuming process and increases the risk of secondary contamination to other healthy cell lines. Mycoplasma...

...recombineering experiment (Figure 2). In the following sections, key steps that differ from traditional restriction...removed from the chromosome of the host E. coli by a second lambda red recombination event. Alternatively, ...