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We narrowed to 482 results for: SEC

Showing: 201 - 220 of 482 results
  1. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...shared server. The blue box labeled “A5” on the second rack in the corner freezer sounds easy to remember... some things I could pay attention to, so my dissections go smoother next time?” or more generally “How...
  2. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...results of any experiment I’ve run in under thirty seconds.” That’s exactly what you would want to talk about...possible, send it to a mentor or colleague for a second look and helpful feedback!  What if I’m not a good...
  3. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ... google “how to write a scientific review.” The second thing I did was stare in horror at the limited ...paper. I also learned to appreciate the discussion sections of the primary literature as a resource that both...
  4. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...include surgery to remove cancerous masses (tumor resection), systemic chemotherapy to kill cancerous cells...added to the treatment recommendations for non-resectable mesothelioma by the National Comprehensive Cancer...
  5. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...salts may be preferable if the protein product is secreted to the medium or if slow expression is required...required due to solubility concerns. Insoluble and Secreted Proteins: The most common purification protocols...
  6. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...Joachim Goedart, an assistant professor at the Section of Molecular Cytology and van Leeuwenhoek Centre...Joachim Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre...
  7. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...aeruginosa. These toxins were a mix of Type VI secreted effector toxins, toxin-antitoxin type toxins, ...Importantly, to prevent activation of toxins before the second crossover step, they tested their expression under...
  8. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ... (Nilaratanakul et al., 2017) and levels of the second messenger cyclic di-AMP in Gram-positive bacteria...fluorescent biosensors for live cell imaging of second messengers cyclic di-GMP and cyclic AMP-GMP." Journal...
  9. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...multiple plasmids. For simplicity, we’ll use the second-generation system as an example.  The lentiviral...remember: You can never trust a lentivirus with secrets... Because they tend to insert themselves into ...
  10. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ... experiments, biotin is added to a primary or secondary antibody that targets your protein of interest... AviTag expressing proteins in bacteria, yeast, insect and mammalian cells. Check out Kay et al., 2009...
  11. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...usually ensure that the 3’-base(s) do not form secondary structures. The introduction (or ablation) of ...around 3% final concentration). The DMSO reduces secondary structures of the DNA template, and may also decrease...
  12. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...through cytotoxicity or the immune response. The second strategy is the simple removal of the HIV-1 genome...to ablate a well-established viral reservoir. A second challenge for CRISPR HIV-1 therapies is sequence...
  13. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...of biting my tongue and gratefully moved on. My second rotation was in a big lab with a famous PI who ... I’ll highlight some of these questions in the sections below. Mentoring relationships can help identify...
  14. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    Published
    Oct. 2, 2025, 1:15 p.m.
    ...several applications of this technology. In each section below, we cite the original paper but link to plasmids...factors present unique challenges for study. These secreted signaling proteins regulate vital cell and tissue...
  15. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...#198003)) and Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Thermo Fisher A-21131... or Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (Thermo Fisher A-11012...
  16. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ... the first number refers to the genome, and the second number to the capsid. Most vectors provided by ...Issa et al., 2023). AAV2 Despite being isolated second, AAV2 is the best studied and most commonly used...
  17. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ... that we describe in the Materials and Methods section of our paper (1). Lastly, it is possible to build...sequencing as described in the Material and methods section of our paper (1). How might one troubleshoot GSRMs...
Showing: 201 - 220 of 482 results