We narrowed to 478 results for: SEC

... google “how to write a scientific review.” The second thing I did was stare in horror at the limited ...paper. I also learned to appreciate the discussion sections of the primary literature as a resource that both...

...include surgery to remove cancerous masses (tumor resection), systemic chemotherapy to kill cancerous cells...added to the treatment recommendations for non-resectable mesothelioma by the National Comprehensive Cancer...

...salts may be preferable if the protein product is secreted to the medium or if slow expression is required...required due to solubility concerns. Insoluble and Secreted Proteins: The most common purification protocols...

... experiments, biotin is added to a primary or secondary antibody that targets your protein of interest... AviTag expressing proteins in bacteria, yeast, insect and mammalian cells. Check out Kay et al., 2009...

...Joachim Goedart, an assistant professor at the Section of Molecular Cytology and van Leeuwenhoek Centre...Joachim Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre...

...aeruginosa. These toxins were a mix of Type VI secreted effector toxins, toxin-antitoxin type toxins, ...Importantly, to prevent activation of toxins before the second crossover step, they tested their expression under...

...in a single step and serve as donors for two consecutive CRISPR-mediated HDR events which results in the...target site after the first HDR event, then the second HDR event can’t occur. Lastly, depending on its...

...multiple plasmids. For simplicity, we’ll use the second-generation system as an example.  The lentiviral...remember: You can never trust a lentivirus with secrets... Because they tend to insert themselves into ...

... (Nilaratanakul et al., 2017) and levels of the second messenger cyclic di-AMP in Gram-positive bacteria...fluorescent biosensors for live cell imaging of second messengers cyclic di-GMP and cyclic AMP-GMP." Journal...

...usually ensure that the 3’-base(s) do not form secondary structures. The introduction (or ablation) of ...around 3% final concentration). The DMSO reduces secondary structures of the DNA template, and may also decrease...

...through cytotoxicity or the immune response. The second strategy is the simple removal of the HIV-1 genome...to ablate a well-established viral reservoir. A second challenge for CRISPR HIV-1 therapies is sequence...

... the first number refers to the genome, and the second number to the capsid. Most vectors provided by ...Issa et al., 2023). AAV2 Despite being isolated second, AAV2 is the best studied and most commonly used...

...of biting my tongue and gratefully moved on. My second rotation was in a big lab with a famous PI who ... I’ll highlight some of these questions in the sections below. Mentoring relationships can help identify...

...several applications of this technology. In each section below, we cite the original paper but link to plasmids...factors present unique challenges for study. These secreted signaling proteins regulate vital cell and tissue...

...#198003)) and Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Thermo Fisher A-21131... or Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (Thermo Fisher A-11012...

... that we describe in the Materials and Methods section of our paper (1). Lastly, it is possible to build...sequencing as described in the Material and methods section of our paper (1). How might one troubleshoot GSRMs...

...Conference highlights included remarks by the Spanish Secretary of Research, Development, and Innovation, D. Carmen...quantifiable, but always significant. Doubts, fears, insecurities… all shrink when shared with a mentor who has...

...of the techniques listed below and always use a second assay to be 100% sure. Before testing for mycoplasma...time-consuming process and increases the risk of secondary contamination to other healthy cell lines. Mycoplasma...

...complex samples such as whole cell lysate or tissue sections. It is important to confirm that your antibody...doi.org/10.1080/19420862.2015.1100787  Taussig MJ, Fonseca C, Trimmer JS (2018) Antibody validation: A view...

... but with random combinations of the different sections. Structure-guided directed evolution If you desire...antibodies that target families of cell surface and secreted proteins (glypicans, integrins, and more coming...