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Showing: 201 - 220 of 481 results

  1. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...any special reagents. However, there’s limited sensitivity at low concentrations of DNA and it can’t distinguish...nm. This method is time consuming and has low sensitivity so it’s not used much. However, the measurements...

  3. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ... including their potential toxicity and cross-reactivity with other pathways. Since these effectors are...chromosomally. Leakiness was also an issue; some promoter activity persisted in the inactive state. The dynamic range...

  4. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...from AAV2 because they demonstrate high promoter activity (Earley et al. 2020), but non-AAV2 ITRs can be...regions of inverted terminal repeats reduces rAAV productivity but increases transgene expression. Sci Rep,...

  5. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...clever system for reducing the on-target off-tumor activity of CAR–T cells was recently devised by the Yingxiao... plasmids here!   Wu Y, et. al. Control of the activity of CAR-T cells within tumours via focused ultrasound...

  6. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ... inserted GFP. After characterizing iGluSnFR in vitro, showing that it is selectively activated by glutamate...they showed that iGluSnFR could detect neuronal activity in C. elegans, zebrafish, and mice. Second Generation...

  8. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...impacted by a project. Instead, invite the experts on the topic at hand. Invite one “ambassador” from each ...

  9. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ... instead of having all the pages in a Curricula Vitae to showcase your publications, academic performances...perhaps you were involved in some extracurricular activities – did you volunteer to do science outreach and...

  11. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    .... Envelope gene (may be pseudotyped to alter infectivity): The use of the VSV-G envelope provides the ...tropism envelope protein; Pseudotyped to alter infectivity. Packaging Gag Precursor structural protein...

  12. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit’s lab. It contains an ampicillin resistance marker...the lacZ gene, thus disrupting 𝛽-galactosidase activity. These cells, presumably the cell you want, cannot...

  13. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...study the effect of gene knockout in vivo and in vitro, as well as to insert targeted mutations through...gRNAs may be used to target genes with overlapping activity. Multiple gRNAs can also facilitate deletions ...

  14. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ... of Ku and normal NHEJ activity. Other factors can also influence NHEJ activity. For example, repressing...

  15. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...How do I engage my students in real-world lab activities? I’m trying to inspire them and also keep up ...extracts to perform transcription and translation in vitro, and the new BioBuilder module allows students to...

  16. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...existing components are adapted and combined with creativity and feedback from the community to create state-of-the-art...Madagascar. Platforms that allow for on-going creativity with the construction plans. Appropriate open-source...

  17. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...probes, with sufficient signal for in vivo and in vitro imaging. Saito et al. used this Nano-lantern to ...intravenously with coelenterazine, with increased sensitivity and faster imaging compared to previous publications...

  18. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...showing that Cpf1 can cleave an array of 4 crRNAs in vitro and when expressed in 293 cells. This allows for...effective method with multiple applications (in vitro, in vivo, transfection, viral transduction) for ...

  19. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...commonly employed mismatch nuclease assays. Sensitivity: Less than 20 ng of total gDNA is sufficient ...Findlay, S. D., Vincent, K. M., Berman, J. R. & Postovit, L.-M. A Digital PCR-Based Method for Efficient...

  20. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...work. After all, as we like to sayat Addgene, productivity is infectious. Curious which viruses researchers...mCherry can be used to induce neuronal silencing in vitro and in vivo (Krashes et al., 2011). The various ...
Showing: 201 - 220 of 481 results