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We narrowed to 800 results for: cha;

Showing: 201 - 220 of 800 results

  1. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...research. This includes chat apps like Slack, Microsoft Teams, and Google Hangouts Chat, resources like Github...an electronic environment and hence making it searchable and shareable, but some ELNs provide better support... delve deeper and look specifically at the characteristics and capabilities an ELN needs to facilitate...send documents from RSpace to a selected Slack channel for discussion and review. It’s also possible to...many different ELNs out there can help with searchability, record-keeping, and data sharing, not all of...

  2. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...sensitivity Longer protocolIncreased chance of cross-reactivityChallenging to develop assay Competitive...antibodies. Assay development, however, can be challenging since scientists must first identify two independent...Disadvantages Direct Fast and simple protocolReduced chance of cross-reactivity Higher level of background...availability Longer protocolLess specificityIncreased chance of cross-reactivity Sandwich High specificityHigh...Competitive Appropriate for small moleculesReduced chance of cross-reactivity Less sensitivityLess specificityLimited...

  3. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ... LA, Garcia KF, Barnes KG, Chak B, Mondini A, Nogueira ML, Isern S, Michael SF, Lorenzana I, Yozwiak NL...reporter is added to the reaction. Cleavage of the quenchable fluorescent RNA by the “activated” Cas13 produces...Csm6 specific reporter (in the same fluorescent channel as the Cas13 reporter), they could significantly...Donghia N, Daringer NM, Freije CA, Myhrvold C, Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti... Koonin EV (2015) Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems. Molecular...

  4. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...ideally less than 10 bp away. For small (<50 bp) changes, you should consider using ssDNA oligo as donor...80 bp of homology arms on each side flanking the change you'd like to introduce. We use Ultramer oligo ...the Cas9/sgRNAs vector (like PX330). For large changes (>100 bp insertions or deletions), we typically...to mutate the PAM 'NGG' in the HR template, like change it to 'NGT', or 'NGA', or 'NGC' (if it's within...required for successful targeting and cleavage, changing it to 'NGT' or 'NGC', in addition to mutations...typically use PAGE purified long oligos. For large changes (>100 bp insertions or deletions), the Zhang lab...doi.org/10.1126/science.1231143  Elliott, B., Richardson, C., Winderbaum, J., Nickoloff, J. A., & Jasin...

  5. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...genome editing without a nuclease Although the mechanism of incorporation is not well understood, researchers...double strand break repair, it has proven more challenging to develop high efficiency CRISPR-based approaches...Schofield, 2015; Moser and Hirsch, 2016; Epstein and Schaffer, 2017) Hopefully you’ve been able to check out...research currently focuses on determining the mechanisms and consequences of cohesin gene inactivation...27280971. 10. Epstein, Benjamin E., and David V. Schaffer. "Combining engineered nucleases with adeno-associated...Medicine, CRISPR, and Genome Engineering. Springer, Cham, 2017. 29-42. PubMed PMID: 29130152. PubMed Central...

  6. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...: In one of my favorite books, Michael Ende’s Momo, the title character endears herself to the nearby ...lack thereof): Information sharing gets more challenging the bigger an organization gets, and creating...communication that gets sent out through multiple channels is not reasonable or sustainable. That said, when...village (and to the reader) by simply listening. Chances are, you know someone like this, even if you’re...these people yourself.  Fig 1: Quote from Michael Ende, courtesy of morefamousquotes.com A good...

  7. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ... of the fly (Pfeiffer et al., 2010). Yeast (Saccharomyces cerevisiae) Yeast, one of the simplest eukaryotic...as Parkinson’s disease (PD). PD is primarily characterized by protein misfolding that leads to a build-...invaluable in understanding the basic molecular mechanisms underlying the disease (Menezes et al., 2015)... development, cell signaling, aging, and mechanotransduction to name a few. For example, Andrew Leifer....5 (2011): 190-195. Liu, Wei, et al. "From Saccharomyces cerevisiae to human: The important gene co‑expression... PubMed PMID: 26712014. Pandey, Udai Bhan, and Charles D. Nichols. "Human disease models in Drosophila...

  8. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...Stochastic multicolor labeling is a popular technique in neuroscience and developmental biology. This... persistently fluorescent during development. Stochastic multicolor labeling systems, many based on Brainbow...even axons of up to 100 microns in length. Some challenges of the Brainbow system are that it does not use...for improving the level of detail possible for stochastic multicolor labeling of the brain. Labeling Drosophila... researchers who developed Tetbow. Conclusion Stochastic multicolor cell labeling systems allow single...Harbor Protocols 2011.1 (2011): pdb-prot5546. Richardson, Alexander, et al. "Visualizing the Fate of Transplanted...

  9. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...nature. Once the bond is formed it is unaffected by changes in pH, temperature or organic solvents. Uses of...it is added to. Biotin also has an exposed side chain that can be easily manipulated to make reactive ...vivo. Biotinylation reagents Biotin’s exposed side chain can be cross-linked to amino acid functional groups... terminus of a DNA sequence during a Polymerase Chain Reaction (PCR) by using a biotinylated primer. Biotin...substrate (Holden and Cremer, 2003). References 1. Chapman-Smith, A., & Cronan, J. E. “Molecular Biology of...129 (2009a): 477–484. PubMed PMID: 10064313. 2. Chapman-Smith, A., & Cronan, J. E. “The enzymatic biotinylation...

  10. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...to simultaneously test how multiple amino acid changes affect a protein of interest’s function. This technique...combination and possibly discover unexpected epistatic changes in protein activity. See Natarajan et al for an...mutagenic primers to introduce all desired codon changes (step 1 in the image above). A second universal...This results in the final product having a codon change present on both strands of the plasmid. The ssDNA...in your research? References 1. Natarajan, Chandrasekhar, et al. "Epistasis among adaptive mutations ... Mccullum, E. O., Williams, B. A., Zhang, J., & Chaput, J. C. (2010). “Random Mutagenesis by Error-Prone...

  11. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...may be familiar with popular light-sensitive ion channels used to control activities like neuronal signaling...activity. Researchers have designed a variety of photoswitchable elements into many other types of proteins....under blue light BcLOV4 undergoes a conformational change and binds the plasma membrane under blue light ...The authors demonstrated its use to probe cell mechanics, vesicle transport, and cell division, but given...family signaling pathways. Rho guanine-nucleotide exchange factors (GEFs) activate their partner Rho GTPase...formation, cell contraction, or other morphological changes, depending on the specific Rho GTPase/GEF pair ...

  12. Addgene Stands with the Scientific Community

    Type
    Blog Post
    Published
    April 23, 2025, 1:30 p.m.
    ...and increased tariffs present an unprecedented challenge to the scientific research ecosystem, not just...concerned about these developments. These abrupt changes threaten to slow transformative biological discoveries...years to come. We Are Here to Help Despite these challenges, Addgene remains steadfast in our mission to ...resilience and creativity in the face of funding challenges. By strengthening our commitment to collaboration... invite researchers to share how these funding changes are impacting your work and what support would ...

  13. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ... technology was invented to introduce specific changes into the mouse genome. With these early technologies...Mice. My first CRISPR mouse experiment Everything changed with the advent of CRISPR in 2013 (Cong et al.,...CRISPR-Cas9 is an acquired immune system in bacteria and archaea. As you know well if you’ve been following the ...sends out an “SOS” signal and cellular repair mechanisms rush in to repair the damage. If all they can...form of an oligonucleotide or plasmid), precise changes can be made in the genome via the homology directed...repair pathway (HDR), be it a single nucleotide change, insertion of a reporter gene, or replacement of...

  14. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...Magenta. Or you can use Color>Split Channels and then Color>Merge Channels into whatever combination of colors...antibody Secondary antibody SCN1A sodium channels anti-pan-Nav R-mAb K58/35R (Mouse IgG2a) Goat... (Mouse IgG1) Goat anti-mouse IgG1 Kv2.1 channel anti-Kv2.1 KC (Rabbit IgG polyclonal) Goat ...images of each labeled target as different color channels and analyze the results. Depending on the goal...rather than red-green). Even better, show each channel in individual panels, then the multicolor overlay...

  15. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...alterations in gene expression, but causality and mechanisms remain unclear. Today, targeted epigenetic modification...could identify the target genes of previously uncharacterized enhancers. The popular TALEN-based LITE system...H3K27ac histone modification at specified loci. Charles Gersbach’s lab has deposited mammalian expression...sorting and selection of transduced cells. Grant Challen’s lab also created constitutive (pCMV-dCas9-D3A)...publication at Addgene. Maeder ML, Angstman JF, Richardson ME, Linder SJ, Cascio VM, Tsai SQ, Ho QH, Sander...Fowler T, Rees R, Kramer A, Martens A, Edwards JR, Challen GA (2016) Reprogrammable CRISPR/Cas9-based system...

  16. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...known about bioluminescent organisms and their characteristic radiance for some time – there are records ...after that, in 1994, Prasher’s collaborator Martin Chalfie expressed GFP in exogenous organisms (E. coli, ...and later C. elegans) for the first time. After Chalfie’s work, scientists really started to take notice... Roger Tsien resulted in much of our current mechanistic understanding of GFP’s function. His lab described...Nobel Prize in chemistry was awarded to Shimomura, Chalfie, and Tsien for their work on GFP. This great honor...

  17. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    Published
    Aug. 13, 2020, 1:21 p.m.
    ... some challenges during my internship, but nothing could have prepared me that those challenges would ...always been interested in the ways that I could change the world around me. My strong interest in the ...exploring my different interests but also a way of challenging what I am used to. Brandon Solis, marketing...structured career because our mindsets and needs can change. Having been at Addgene for a month, I can say ...

  18. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins by Cary Valley, Shreya Vedantam, Michelle...fluorescent proteins at Addgene. 7. Designing Your Chalk Talk for the Academic Job Interview by Erik Snapp...blogger Erik Snapp breaks down how to prepare a chalk talk for an academic job interview including defining... research vision, organizing your vision into a chalk talk, and tips for practicing.  6. The Fluorescent...insertions, deletions, and all possible base-to-base changes.   1. Plasmids 101: Transformation, Transduction...

  19. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...from Zygosaccharomyces rouxii), B2 (from Zygosaccharomyces bailii) and B3 (from Zygosaccharomyces bisporus...as low toxicity. FLP/FRT recombinases (from Saccharomyces cerevisiae) had been used successfully in Drosophila... Kotelevtseva N, King K, Davis JR, Mullins LJ, Chapman KE, Dunbar DR, Mullins JJ. Nucleic Acids Res. 2007...

  20. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...selection. Knock-in events tend to be rare, and the challenge with any experiment is in efficient recovery of...increase in knock-in efficiency, presumably by channeling DNA breaks into the homologous recombination ... template design The Meyer lab meticulously characterized several aspects of editing. First, they devised...injected animal.  PAM sequence considerations For changes 5’ to the PAM, sequences corresponding to the same...Prize included Jennifer Doudna and Emmanuelle Charpentier for their pioneering CRISPR/Cas9 work (now recognized...proteins associated with a given genomic locus (CRISPR-ChAP-MS) could further transform the range of experiments...
Showing: 201 - 220 of 800 results