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Showing: 201 - 220 of 382 results
  1. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...glycerol stocks, prepped DNA ready for our quality control processes - all of these samples have the 2D barcodes...along the way involves good aseptic technique and controls to ensure that nothing is contaminated. We use...
  2. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ... Plasmid 2: Magneto 2.0 form Ali Guler's Lab - Control neuronal activity with a magnetic field. Listen...
  3. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...example of a FRET experiment with all relevent controls near the end of the post also really helped put...
  4. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...positive control in our knock-in experiments as it works quite efficiently and serves as a good control for...antibody.Note: Always include a positive and a negative control for the tagging experiment and test them in parallel...
  5. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...Technology Austria, decided to take a novel approach to control for these effects, drawing on the rapidly growing...also available at Addgene and can be used as a control for your experiments. For more information, please... allowing labs to precisely and non-invasively control neuronal signaling. For more information on DREADDs...viral (AAV) system enables greater spatio-temporal control over expression and increases the number of species...Thy1 or CAG promoter, while Brainbow AAV is under control of the EF1a promoter. Livet et al., Nature. 2007...
  6. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...nonprofit? Get an Inside Look at Addgene's NGS Quality Control Addgene's Viral Service - Why Virus? Why Now? ...
  7. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...favoring of certain types of mutations, difficulty controlling the rate of mutagenesis, and low throughput. ...combination of library coverage, mutational efficiency, control over where mutations are introduced, and scalability...
  8. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...a large number of requests for vectors in the “controls” category, which includes things like viral vectors...Patrick, M., Guerin, K., & Fan, M. (2023). Quality control for Adeno-associated viral vector production. Neuromethods...
  9. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...1998). Regulatory elements within the ori also control copy number and stability, which influence the ...which consist of a plasmid’s ori and all of its control elements, from broad host range plasmids can be...
  10. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...toxicity or burden relative to a fluorescent protein control. We also added the toxin-antitoxin stability cassette...Biomolecular Tools for Acoustic and Magnetic Imaging and Control of Cellular Function. Biochemistry 56, 5202–5209...
  11. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ... direct shipments weren’t able to clear import control. Partnering with a distributor allowed us to share... systems to allow for easier access to export-controlled items by streamlining permissions and paperwork...
  12. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...Weissman JS (2014) Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation. Cell 159:647...2015) A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol 11...
  13. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...approach complete with freely available positive controls. Routinely test your cell line for mycoplasma ...Cleghorn G., West M.J., Wei MQ. “The significance of controlled conditions in lentiviral vector titration and...
  14. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ...your plasmids, storing them, providing quality control and customer support, making plasmids easy for ...
  15. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ... of knockdown and developed new, more tightly controlled, Tet responsive promoters to avoid leaky activation...This approach provides a more granular level of control where individual bases can be modified. Cytidine...as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell 152:1173–1183 . https:...
Showing: 201 - 220 of 382 results