We narrowed to 430 results for: lias

...please do not hesitate to email me at snappe@janelia.hhmi.org." You can find his original blog post below...Director of Student and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute...

...aExtinction coefficient. bQuantum yield. cDetermined in mammalian cells. dDetermined as effective NIR fluorescence...Brightness of Near-Infrared Fluorescent Proteins in Mammalian Cells. Cell Chem Biol 24, 758-766 e753 (2017)....

...deliver the alkaline phosphatase gene to airway epithelial cells in mice. This vector transduced ~10% of...A, Rex TS, Di Vicino U, Cutillo L, Sparrow JR, Williams DS, Bennett J, Auricchio A (2008) Serotype-dependent...

...facilitate gene expression in bacteria, yeast, mammalian cells, insects, and even Xenopus oocytes. The ...segments of choice into either an E. coli or a mammalian expression vector in a one-pot reaction. Using...

...HIV-1 latent T cell lines, as well as a latent microglial line, treatment with CRISPR/Cas9 SAM increased...Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang C. CRISPR/Cas9-Derived Mutations Both Inhibit HIV...

...of endogenous light-sensitive receptors, like mammalian rhodopsin, and the intracellular domain of an ..., Papagiakoumou, E., Gottschalk, A., Soba, P., Emiliani, V., Engel, A. K., Hegemann, P., & Wiegert, J....

...promoters Selective promoters, such as synapsin, glial fibrillary associated protein (GFAP), or tyrosine-hydroxylase...Zingg, B., Chou, X. lin, Zhang, Z. gang, Mesik, L., Liang, F., Tao, H. W., & Zhang, L. I. (2017). AAV-Mediated...

...successful implementation of GRSMs depends on the reliability of the parts used to build them. We recommend...23034349. PubMed Central PMCID: PMC3914141. 5. Brown, William RA, et al. "Serine recombinases as tools for genome...

...successfully used for genetic manipulation in mammalian cells until 2005 [1]. Like other transposons, ...Functional Gene Correction for Cystic Fibrosis in Lung Epithelial Cells Generated from Patient iPSCs.,” Cell Rep...

...Director of Student and Postdoctoral Programs at theJanelia Research Campus of the Howard Hughes Medical ...Director of Student and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute...

...optical and genetic methods to photo-stimulate mammalian neurons and alter synaptic events: a new technology...Diester. "Optogenetic approaches to study the mammalian brain." Current opinion in structural biology ...

...development of viral vectors, gene transfer into mammalian cells often relied on chemical (CaCl2, cationic... differentiated cells such as macrophages and microglia. This property makes the lentivirus an attractive...

...CRISPR/Cas9 editing strategies in C. elegans As brilliantly and irreverently illustrated in the parable of...innovation for our community. Developments in mammalian and yeast cells using modified Cas9 proteins to...

...or universal. This method is quick, efficient, reliable and cost-effective (Sung, 2006). The sensor cell-based...) Sensitive Assay for Mycoplasma Detection in Mammalian Cell Culture. Anal Chem 84:4227–4232 . https:/...

... genetic inheritance (1866) and just when the Mendelian genetics was being revived by the botanist Hugo...engineering itself is attributed to SciFi writer Jack Williamson who used it in his 1951 novel Dragon’s Island...

...experiments, experience has shown that some are more reliable and more efficient at tracking cells. Good two-photon...extension into the small bowel lumen in response to epithelial cell TLR engagement." Journal of Experimental...

...cell types, especially immune cells and cells of epithelial origin. Suppressing function, creating tolerance...various signals. Over-production of vascular endothelial growth factors (VEGF) in tumors is attributed...

...basic biology of optogenetics so if you are not familiar with it, check out the video below). That’s not...coordinates at bregma which allows you to quickly and reliably locate your injection site. We recently purchased...

... available in your lab or the one you are most familiar with. And, hey — if something is working for you...high quantity) – Depending on the experiment, mammalian cell transfections can require quite large amounts...

...identified a protein that enhances prime editing in mammalian cells: the small RNA-binding exonuclease protection...Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases and...