Skip to main content
Addgene
Showing: 201 - 220 of 419 results

  1. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...of endogenous light-sensitive receptors, like mammalian rhodopsin, and the intracellular domain of an ..., Papagiakoumou, E., Gottschalk, A., Soba, P., Emiliani, V., Engel, A. K., Hegemann, P., & Wiegert, J....

  2. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...promoters Selective promoters, such as synapsin, glial fibrillary associated protein (GFAP), or tyrosine-hydroxylase...Zingg, B., Chou, X. lin, Zhang, Z. gang, Mesik, L., Liang, F., Tao, H. W., & Zhang, L. I. (2017). AAV-Mediated...

  3. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...AAV5 is generally the best choice for targeting astroglia. It has additionally been reported to have tropism...tropism for lung, liver, vascular endothelial, and smooth muscle cells (Issa et al., 2023). AAV5 capsids...

  4. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...successful implementation of GRSMs depends on the reliability of the parts used to build them. We recommend...23034349. PubMed Central PMCID: PMC3914141. 5. Brown, William RA, et al. "Serine recombinases as tools for genome...

  5. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...successfully used for genetic manipulation in mammalian cells until 2005 [1]. Like other transposons, ...Functional Gene Correction for Cystic Fibrosis in Lung Epithelial Cells Generated from Patient iPSCs.,” Cell Rep...

  7. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ... genetic inheritance (1866) and just when the Mendelian genetics was being revived by the botanist Hugo...engineering itself is attributed to SciFi writer Jack Williamson who used it in his 1951 novel Dragon’s Island...

  9. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...development of viral vectors, gene transfer into mammalian cells often relied on chemical (CaCl2, cationic... differentiated cells such as macrophages and microglia. This property makes the lentivirus an attractive...

  10. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...Director of Student and Postdoctoral Programs at theJanelia Research Campus of the Howard Hughes Medical ...Director of Student and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute...

  11. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...CRISPR/Cas9 editing strategies in C. elegans As brilliantly and irreverently illustrated in the parable of...innovation for our community. Developments in mammalian and yeast cells using modified Cas9 proteins to...

  13. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...cell types, especially immune cells and cells of epithelial origin. Suppressing function, creating tolerance...various signals. Over-production of vascular endothelial growth factors (VEGF) in tumors is attributed...

  14. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...basic biology of optogenetics so if you are not familiar with it, check out the video below). That’s not...coordinates at bregma which allows you to quickly and reliably locate your injection site. We recently purchased...

  15. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ... available in your lab or the one you are most familiar with. And, hey — if something is working for you...high quantity) – Depending on the experiment, mammalian cell transfections can require quite large amounts...

  16. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...identified a protein that enhances prime editing in mammalian cells: the small RNA-binding exonuclease protection...Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases and...

  17. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...Overall, integrating viral vectors efficiently and reliably generate iPSCs, but they have several safety concerns... and allows for replication of the plasmid in mammalian cells and assists in tethering the vector to the...

  18. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ..., qPCR needs a steady pair of hands to produce reliable and reproducible data. Here are some tips to help... you’ve done the work to produce excellent and reliable qPCR data, you’ll of course want to share it so...

  19. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...jacket, home to a questionable collection of memorabilia, even made an appearance during co-founder and...

  20. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...gave to me: a miniature CRISPR-Cas system for mammalian use. On the second day of CRISPR, Addgene gave...
Showing: 201 - 220 of 419 results