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We narrowed to 430 results for: lias

Showing: 201 - 220 of 430 results
  1. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...please do not hesitate to email me at snappe@janelia.hhmi.org." You can find his original blog post below...Director of Student and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute...
  2. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...aExtinction coefficient. bQuantum yield. cDetermined in mammalian cells. dDetermined as effective NIR fluorescence...Brightness of Near-Infrared Fluorescent Proteins in Mammalian Cells. Cell Chem Biol 24, 758-766 e753 (2017)....
  3. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...facilitate gene expression in bacteria, yeast, mammalian cells, insects, and even Xenopus oocytes. The ...segments of choice into either an E. coli or a mammalian expression vector in a one-pot reaction. Using...
  4. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...HIV-1 latent T cell lines, as well as a latent microglial line, treatment with CRISPR/Cas9 SAM increased...Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang C. CRISPR/Cas9-Derived Mutations Both Inhibit HIV...
  5. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...of endogenous light-sensitive receptors, like mammalian rhodopsin, and the intracellular domain of an ..., Papagiakoumou, E., Gottschalk, A., Soba, P., Emiliani, V., Engel, A. K., Hegemann, P., & Wiegert, J....
  6. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...promoters Selective promoters, such as synapsin, glial fibrillary associated protein (GFAP), or tyrosine-hydroxylase...Zingg, B., Chou, X. lin, Zhang, Z. gang, Mesik, L., Liang, F., Tao, H. W., & Zhang, L. I. (2017). AAV-Mediated...
  7. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...successful implementation of GRSMs depends on the reliability of the parts used to build them. We recommend...23034349. PubMed Central PMCID: PMC3914141. 5. Brown, William RA, et al. "Serine recombinases as tools for genome...
  8. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...successfully used for genetic manipulation in mammalian cells until 2005 [1]. Like other transposons, ...Functional Gene Correction for Cystic Fibrosis in Lung Epithelial Cells Generated from Patient iPSCs.,” Cell Rep...
  9. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...Director of Student and Postdoctoral Programs at theJanelia Research Campus of the Howard Hughes Medical ...Director of Student and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute...
  10. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...development of viral vectors, gene transfer into mammalian cells often relied on chemical (CaCl2, cationic... differentiated cells such as macrophages and microglia. This property makes the lentivirus an attractive...
  11. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...CRISPR/Cas9 editing strategies in C. elegans As brilliantly and irreverently illustrated in the parable of...innovation for our community. Developments in mammalian and yeast cells using modified Cas9 proteins to...
  12. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ... genetic inheritance (1866) and just when the Mendelian genetics was being revived by the botanist Hugo...engineering itself is attributed to SciFi writer Jack Williamson who used it in his 1951 novel Dragon’s Island...
  13. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...cell types, especially immune cells and cells of epithelial origin. Suppressing function, creating tolerance...various signals. Over-production of vascular endothelial growth factors (VEGF) in tumors is attributed...
  14. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...basic biology of optogenetics so if you are not familiar with it, check out the video below). That’s not...coordinates at bregma which allows you to quickly and reliably locate your injection site. We recently purchased...
  15. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ... available in your lab or the one you are most familiar with. And, hey — if something is working for you...high quantity) – Depending on the experiment, mammalian cell transfections can require quite large amounts...
  16. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...identified a protein that enhances prime editing in mammalian cells: the small RNA-binding exonuclease protection...Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases and...
Showing: 201 - 220 of 430 results