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...re still a bit confused by CRISPR acronyms, this post is for you. We’ll cover the common terminology for...guide or our CRISPR 101 ebook. We also have a blog post on the structural biology of Cas9. Want a simpler...spacer sequence is the RNA guide, like a wanted poster that Cas9 uses to track down its suspect—the target...

...which contains the viral genome. In today’s blog post, we’ll focus on the packaging plasmid and take a...understanding of the AAV RepCap plasmid! Check out this blog post to learn more about the AAV transfer plasmid! ...

...article, a preprint, or without any paper at all. Posting preprints and openly sharing materials are both... to release the hold as soon as the preprint is posted in order to support these goals. And we can update...

...a kit Read our blog posts about plasmid cloning Browse our Plasmids 101 blog posts Resources on Addgene.org...

...Read our blog post about external collaborations Browse our science career related blog posts ...

...needs. If you need an FP refresher, see our FP blog posts.     References Sattarzadeh A, Saberianfar R, Zipfel...Fluorescent Protein Plasmids and Resources Relevant Blog Posts Plasmids 101: Green Fluorescent Protein Which Fluorescent...

...about being an effective manager, read the other posts in this series. Listen to our podcast interview ... on this topic and get the whole series of blog posts in one pdf - download the Addgene Management for...

...The following post was contributed by Daniel Bauer and Matthew Canver of Boston Children’s Hospital and...CRISPR Information at Addgene More CRISPR-Cas Blog Posts    ...

...optional media exchange can be performed 12–18 hours post-transfection to help remove toxic transfection reagents...uses, check out our ddPCR for AAV quantitation blog post.  We generally do not recommend using ELISA-based...applications by reading our Viral Vectors 101 series!  This post was originally written by Didem Goz Ayturk and updated...

...Browse our Fluorescent Protein 101 blog posts Read our blog post covering an introduction to FRET Resources...This post was contributed by Patrick Miller-Rhodes from the University of Rochester Medical Center.  ...

...Plasmids articles. Here's what you'll find in this post: Bright and photostable yellow fluorescent protein...generate heritable edits (learn more in our recent blog post on TnpB). Similar to CRISPR-Cas, TnpB is directed...neuroscience applications (Figure 4). Check out our blog post on AAV serotypes for more about the tissue tropism...released into the synapse. Once AF binds to the postsynaptic AMPA receptor and is endocytosed, the Cre recombinase...

.... To see even more tools, head over to this blog post that highlights five different methods to use CRISPR...behind-the-scenes glimpse of their work, head over to their blog post. Months later, we partnered with Ginkgo Bioworks... remote collaborations Browse all COVID-19 blog posts Resources on Addgene.org Find COVID-19 resources...

...catalogue of viral preps available to request. In this post, we’ll walk you through how we produce both our ... function. These data are sometimes reported or posted on the material page for the corresponding catalog... of early passage cells. Approximately two weeks post-thaw, cell culture supernatant is tested for mycoplasma...

...This post was contributed by Rory Macneil, founder of Research Space. There are many types of electronic...reproduce the research that produced the data. In this post I’d like to delve deeper and look specifically at... Minisymposium on Reproducibility Read all blog posts about scientific sharing Resources on Addgene.org...

...This post was contributed by guest blogger, Jennifer Rivers, a research microbiologist specializing in...prescription for a bacterial infection. While this post will, indeed, focus on bacteria, it is important...bacterium to survive). Check out our recent blog post on CRISPR antimicrobials to learn how scientists...

...This post was contributed by guest blogger Gairik Sachdeva. Healthcare consulting is a fast-paced field...their knowledge to a variety of problems. In this post, I’ll share what I’ve learned as a healthcare consultant...and occasionally being faced with “shifting goal posts” as a part of a case, consulting can sometimes be...

... View Other CRISPR Blog Posts Additional Resources Zhang lab Cpf1 blog post on Benchling ...experimentally confirmed. To prevent new editing post-HDR, repair templates should remove the PAM sequence...

... Resources Read other posts about life at Addgene!Read more of our careers posts!    ...successful event at your company. The point of this post isn’t to make you all jealous that you don’t work...Spread word of your plans by email, gong, evite, posters, megaphone, or your company’s intranet. Don’t forget...

...We've discussed promoters before so in this blog post we’ll cover basics of multicistronic vectors: why... plasmids. We've combined our Plasmids 101 blog posts and additional resources into one downloadable PDF... 2008 May 11.) PubMed.   Read more Plasmids 101 posts or browse Addgene's Plasmid Guide for more molecular...

...are transcribed from the RNAP III promoters. This post features promoters for general RNAP II and RNAP ...constructs and we will discuss these in a future post on viral vector parts. Promoter specificity Aside...Resources on the Addgene Blog Browse All Plasmids 101 Posts Learn about Mammalian Vectors Read about Reporter...