Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
Type
Blog Post
Published
Dec. 8, 2016, 3:30 p.m.
...ligase-mediated method, the more recently developed Gibson Assembly Cloning and Gateway® cloning technologies... toxic protein (CcdB) that acts as a DNA gyrase poison, locking up DNA gyrase with broken double stranded...killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes.” J Mol Biol...