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We narrowed to 243 results for: virus

Showing: 201 - 220 of 243 results

  1. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...out there for extracting DNA from tissues, blood, virus, or other samples, for PCR and gel cleanup, and ...

  4. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...generate lentivirus (B). Cas9-expressing cells or wild-type cells are treated with lentivirus containing... Quick Guide to All Things Lentivirus Get Tips on Titering Your Lentivirus Plasmids 101: NGS Quality Control...genome wide-screening experiments commonly use lentivirus to deliver a pooled population of gRNAs to Cas9... concentration that is sufficient to generate lentivirus. Be sure to use next-generation sequencing to...the steps above! Cells are then transduced with lentivirus containing the CRISPR library to generate a heterogeneous...

  5. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ... But what if we could specifically target only virulent or antibiotic resistant bacteria with a weapon...no antibiotic strategy available to target only virulent or antibiotic resistant bacteria, and inappropriate...antimicrobials can target specific sequences in a single virulent bacterial species, or even an antibiotic resistance...Bikard et al. used a phagemid system to target virulent S. aureus, a common hospital-borne infection. ...phagemid treatment were sufficient to wipe out the virulent strain. Bacteria not killed by the first round...phagemid treatment decreased the proportion of virulent S. aureus from 50% to 11% in just 24 hours.   ...they suggest that targeting multiple resistance/virulence factors may further reduce this likelihood. The...

  6. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...Mammalian Lentivirus pRDA_512 (RSV-driven; lentiGuide-based) Mammalian Lentivirus pRDA_722...lentiCRISPRv2-based; preferred over 512) Mammalian Lentivirus pRDA_789 (CMV-driven; CROPseq) Mammalian...Organism (Mammalian or drosophila) Delivery type (lentivirus, AAV, transposon, Plasmid) Destination vector...

  7. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...requests for access to coronavirus-related plasmids and to receive important coronavirus-related deposits. ...information resource for scientists studying the coronavirus. Addgene’s Technology Transfer Team also moved...

  12. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...copy virulence plasmids. This strategy has been successful in displacing, or “curing” the virulence plasmids...

  13. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...Jaenisch’s lab in plasmid form or as ready-to-use lentivirus. Figure 2: dCas9-Tet1 demethylates cytosines...with the former also available as ready-to-use lentivirus. Figure 3: dCas9-DNMT3A methylates cytosines...

  14. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone...the process of creating complex polycistronic lentiviruses. The MuLE toolbox contains a series of building-block...tRNA/aaRS pairs are delivered via a pseudotyped baculovirus system, which is advantageous compared to other...

  15. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ...them out: https://t.co/uDEE9PsspD#deckthelab #virussnowflakes #sciart #thankyouscience #scicomm pic.twitter.com...

  18. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ... aptamer binding protein for packaging into a lentivirus-like particle. Read the paper in Nucleic Acids...
Showing: 201 - 220 of 243 results