...there’s a PAM near your insertion site, the corresponding gRNA might be undesirable due to potential off-target...than SpCas9, in this case, NNGRRT and NNNNGATT, respectively. Researchers also engineered SpCas9 variants..., resulting in lower overall genome frequency. Despite the progress we’ve made, there is still a need ...

... on which lab jobs needed to be done (Who is responsible for making the running buffer this week?). Scientists...what is most important to each person. It can especially be difficult to think creatively when you are...create a win-win situation? Learning about and respecting cultural differences is a crucial part of understanding...

...are also an important target for gene delivery, especially for the study of pain. While many new capsids...and AAV.PHP.S, that target the CNS or the PNS, respectively.  Read on to learn more about these two new ...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nature biotechnology...

...individual molecules. Poor photon budgets are a widespread issue with fluorescent proteins—even the thriftiest...student searching couch cushions for spare change, desperate to extract a few more photons from a sample. Chemical...have excellent properties inside living cells, especially for advanced imaging experiments. We continue...

...without triggering a destructive inflammatory immune response. At the time, adenoviral vectors were the most...disease-causing genes, including the RPE65 gene responsible for an autosomal recessive, severe, childhood-onset...follow in the footsteps of Luxturna™. However, despite promising results in preclinical studies some potential...

...and it would have been very difficult for us to respond to such a large number of requests by ourselves...with each other, which is critically important, especially for young researchers. It was a really nice incubator...Identification of Proteins Associated with an IFNgamma-Responsive Promoter by a Retroviral Expression System for...

...Quiñones-Valles, C., Espíndola-Serna, L., Martínez-Antonio, A., Quiñones-Valles, C., Espíndola-Serna, L., & Martínez-Antonio...Solar, G., Giraldo, R., Ruiz-Echevarría, M. J., Espinosa, M., & Díaz-Orejas, R. (1998). Replication and...

...when bacterial cells regulate gene expression in response to changes in cell density. At the mixer, they...reviewing and commenting. Binning Singletons is especially grateful to its mentors and supporters during...was that kid who used recycled notebook paper, despite the fact that it was gray. He has worked in molecular...

... suggestions based on what I have found to be especially beneficial during my own graduate career – and...Attend smaller talks within the bigger conference, especially ones that are more career-focused. After these... important to realize that you have to take responsibility for your own professional development, not ...

...facilitate binding and cleavage of target DNA respectively. LshCas13a, on the other hand, uses only a short...(RPA) or Reverse-transcription RPA (RT-RPA), respectively. Amplified nucleotides are combined with Cas...expression. Although the collateral RNA cleavage response is well described in bacterial cells, LwaCas13a...