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We narrowed to 729 results for: NSI

Showing: 111 - 120 of 729 results

  1. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...approaches can be found. As an example, say you are considering either a cellular screen or a selection. A selection...specific to your scientific question. Things to consider when selecting an assay system include: How specific...specific is it? Is it high throughput? Will it be sensitive enough? No one wants to spend time screening hits...t yield any hits. Make sure you plan ahead and consider these questions to save yourself a headache later...scene, there are other options which are worth considering. RNAi screens are similar to CRISPR, but instead...method, popularized in yeast, are temperature sensitive screens. These involve raising or lowering the...

  2. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...with Addgene’s expansion into plant synthetic biology. There are already two comprehensive plant cloning... of the technology to the public in an open, responsible manner. At Revolution Bio, we are addressing ... circadian rhythm in petunias. Because of the extensive research that has come before us, it’s possible...change color. Without this basic research – this comprehensive understanding of the genetic pieces underpinning... intellectual property concerns – patents and licensing agreements. Developing a product without the use...

  3. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ...school and turn it into a biotech company. Transitioning an idea from academia to a startup Mike Koeris... biotech startup, Mike explained, his career transitioned away from the bench and became all about finding...failure at all stages in the startup". To gain insight into "the random-walk" of a budding startup, I ...company won’t go anywhere. The team should initially consist of 2-3 individuals that are aligned in vision and...company may begin to prove the technology. This transition takes around 1 – 2 years. The product is the...into it.” Have any other helpful tips to help transition a project from academia to a biotech startup ...

  4. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...budding. Yeast Centromere plasmids (YCp): These are considered low copy vectors and incorporate part of an ARS...are most similar to bacterial plasmids and are considered “high copy”. A fragment from the 2 micron circle...spontaneously occurring resistant mutants and the insensitivity of yeast strains to some antibiotics. Auxotrophy...uses for said element. This link provides a more extensive list of yeast auxotrophic markers and includes...auxotrophic selection in E. coli? Additional considerations HIS3 L-hisitidine no S. cerevisiae  no yes...ade6+ purine (adenine)   S. pombe  no no   Considerations when using auxotrophic selection Of course,...

  5. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...interested in following? Someone you admire for their insight and way of expressing it in meetings? The more ...help you learn the local “ropes” and become an insider faster A mentor that works elsewhere is able to... If your organization has a mentoring program, consider tapping into it and helping make it work by being... coaches who charge for their advice and time. Consider this option but be cautious and make sure to choose... to be your mentor, it may sound like a big responsibility or commitment. Start by asking them to have...questions and topics to discuss. The mentee is responsible for leading the discussion and for being “coachable...

  6. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...recurrence, and treatment of cancer have been extensively explored. Biologists have attempted to study ...and dynamic phenomenon that is interestingly responsible for tumor reduction as well as progression. Dunn...is a region of incessant chemical activity, and consists of other cell types, especially immune cells and...relapse. Chemical and radiation therapies cause a transient shutdown of the immune system, hampering its ability...have been made to use whole cell therapeutics consisting of various types of immune cells. Dendritic cells...exhibit cytotoxic activity. CAR-T cells have consistently shown promise in reducing leukemia and lymphoma...impacted by the trial. During clinical trials, expansion of participants based on early results can enhance...

  7. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...thin plane within a relatively thicker three dimensional sample (see Figure 1); similar to an MRI or CT...rapid photobleaching and a reduction in signal intensity. 2-Photon microscopes restrict excitation (and...focal point of the objective can a a high enough density of red-photons be establish for this to happen....field of the objective (the distance in the axial dimension that will appear in focus). This means that the... then collected and projected back to a fast, sensitive detector such as a scientific CMOS camera. If ...Reversible Saturable Optical Linear Fluorescence Transitions (RESOLFT/STED) also excel on these types of samples...a host of tools and opened doors to many great insights, it can be overwhelming to a novice microscopist...

  8. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...in vivo, they have higher reproducibility and consistency in the final product. Because the constant (non-epitope...binding) region is the first thing to go when downsizing an affinity reagent, most of the alternative ...its thorns and every affinity reagent has its downsides. These reduced-in-size options often have reduced... as ankyrin repeat proteins. DARPins typically consist of 3-4 repeats, have high specificity and binding...it? This post isn’t meant to convert anyone to downsize their affinity reagents, trendy as that may sound...Grumezescu, A. M. (2018). Drug targeting and stimuli sensitive drug delivery systems. Elsevier Science & Technology...

  9. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...LoxP vector.   Turn it off for a little while Transient suppression of expression is faster and easier...molecules that degrade target mRNA transcripts. When transiently introduced, they can deplete endogenous protein...instead of introducing a DNA double strand break. Transient introduction of CRISPRi or inducible expression...expression. Addgene has a dCas9 KRAB plasmid for transient knockouts!  Turn it on Turn it on forever Permanently...activator and repressor mechanisms Want to transiently induce expression? Check out Addgene's Lentiviral...site-specifically knocked in. This can be labor intensive and time consuming. If your native protein doesn...

  10. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ...experimental design considerations used in all behavioral studies. General considerations for behavioral ...behavioral testing As a general consideration for behavioral testing in a laboratory, a high level of experimental...increase stress levels and affect behavior. A fact to consider: Rodents can hear ultrasonic frequencies (> 20...targeted to a specific area. Some other technical considerations for stereotaxic injections are: Vector titer...brain areas, repeated staggered injections may be considered. Timing: High expression levels of the viral ...
Showing: 111 - 120 of 729 results