We narrowed to 387 results for: abo.5

..., the sacB gene is used for negative selection on 5% sucrose plates. Exchange of the sacB gene for the... 101 blog posts Learn more about Lambda Red recombineering Learn more about counter-selection Resources...levansucrase that converts sucrose into a toxic metabolite in gram-negative bacteria. Plating on sucrose...

...317–334 . https://doi.org/10.1007/s40259-017-0234-5  Büning H, Srivastava A (2019) Capsid Modifications...engineered Learn about the parts of the AAV transfer plasmid  Resources on Addgene.org Learn about AAV Find ...plasmid! Check out this blog post to learn more about the AAV transfer plasmid! References: Naso MF...

...This is the fifth in a 5 part series on Management for scientists.  We are inundated with articles and...that their teams are working well together and collaborating effectively with other teams. While they sometimes... they are as engaged as possible. To learn more about being an effective manager, read the other posts...our podcast interview with Connie Cepko to learn about her management style. Leaders look to the future...

...Dunbar DR, Mullins JJ. Nucleic Acids Res. 2007;35(5):1402-10. doi:10.1093/nar/gkl1108. Epub 2007 Feb 6...site-specific recombination in Drosophila, read on to learn about new recombinases suitable for this system. Gerald...recombinases from the Rubin lab For more information about limitations and caveats of Cre usage: Vagaries of...

...100 µL (1 mL), 25x 100 µL (2.5 mL), or 50x 100 µL (5 mL).  Once submitted, the request will be reviewed...educational resources.  We hope you’re as excited about this expansion as we are! Happy packaging! ...

...Tropism and Axonal Transport of AAV Serotypes 1, 2, 5, 6, 8 and 9 in the Mouse Brain. PLoS ONE 8:e76310....Pseudotyped with Viral Capsids from Serotypes 1, 2, and 5 Display Differential Efficiency and Cell Tropism after...the most common concerns we get here at Addgene about this system is off-target or spurious expression...cells that don’t express Cre or Flp, what can we do about it?  Test the serotype and promoter The first step...

...Learn How You Can Use Video to Share Your Research 5 Reasons to Use Reddit for Science Communication 10...finally decide the LEGO laboratory was good enough. There are no pre-made LEGO laboratory sets so we had to ...everything now is about the plot. This part is easy, after all, who working in a laboratory has never faced...dysregulated water bath boiling your experiments, just about anything can disrupt your bench work and sometimes...to show people the very human struggles in the laboratory routine. We hope that sharing and laughing at...can learn with each other through globalized, collaborative thinking. LEGO and science: The potential is...while opening a LEGO set with my husband, I thought about how the limitless design possibilities of the LEGOs...

...blogosphere is to read and comment on their blogs. 5)   Local science museums are a great place to find...take on collaborative roles in academia, and that means it’s important to know how to talk about science...talking about and sharing some science on the one you already have. Read my earlier post about Scientists...important for scientists to get out there and talk about their work. To tell others why that work is cool... than ever before for scientists to communicate about their work and their interest in science more generally...already make it a habit to talk – really talk – about the research you are doing in the lab with your ... the details of your science but also something about how science really works. 2)   Read science writing...

...transgene. On a plasmid map, promoters are upstream or 5’ of the gene they control. Promoters can be organism...the Addgene blog Learn more about basic viral vector elements Read about four considerations for the ...Key elements of an AAV plasmid First let’s talk about the key parts of an AAV plasmid. Inverted terminal...routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ITR loss, it’s best... Other cis-regulatory elements Next let’s talk about some of the elements that aren’t required parts ...the perfect AAV tool Learn about synthetic promoter AAVs for cell-type expression in retinal cells Resources...

...bioengineering 37.10 (1991): 989-993. PubMed PMID: 18597326. 5. Shen, Chun Fang, and Amine Kamen. "Hyperosmotic pressure...resources on Addgene.org Learn about Addgene's viral service Learn about viral production Find viral protocols... By now you have probably heard about Addgene’s viral service but did you know that Addgene also performs... approach can be adapted by vector cores and laboratories alike as an easy and inexpensive method to improve...Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production." Biotechnology and ...

...Biorender workspace. Free Biorender accounts can store 5 workspaces at a time and workspaces are only downloadable... for the Addgene blog, I was focused on writing about new scientific techniques and cool plasmids. Creating... graphics were usually the last thing I thought about when writing posts. Since then I’ve realized my ...know of a tool or resource not mentioned? Tell us about it in the comments section!  Additional resources...scientists Find science career blog posts Learn about other lab software ...

... The sample was separated into smaller samples of 5-10 cells each for sequencing, allowing for lower complexity...Addgene blog Learn about other Cas proteins Read all of our CRISPR blog posts Read about GeoCas9, another... able to eliminate the need for axenic (pure) laboratory cultures to study these microbes. From 16S rRNA...by flexibly deploying IgnaviCas9 without concern about its stability under harsh conditions. One example...

...Blue-White screening could be a potential solution. 5. Cpf1: A New Tool for CRISPR Genome Engineering While...eyes open for any new technologies that will bring about the next revolution in biological research. We’re...Plasmids 101: Control Plasmids Need help thinking about the types of controls you’ll need for your next ...Addgene Browse Our CRISPR Resources Learn More about Viral Vectors Browse the Repository  ...

...you choose the best CRISPR software for your needs 5 great apps for lab life  Sharing your lab protocols...particular tasks, but they tend to be scattered about the internet. To help you keep your virtual molecular...other applications Primer design tools Many of the above-listed plasmid mapping tools have built-in primer...

... patterns generated by the nickases; D10A creates 5’ overhangs and H840A creates 3’ overhangs in a PAM-out...Additional Resources on the Addgene Blog Learn about Homology Directed Repair 3 Tips to Improve HDR Efficiency...

...proto-oncogene product. Molecular and Cellular Biology, 5(12), 3610–3616. doi: https://doi.org/10.1128/mcb.5.12.3610...Brian O'Neill  The François St-Pierre lab and collaborators have successfully developed an improved voltage...

...increased my autonomy and satisfaction with my work.  5. Do you enjoy bench work? It’s easier to stay at the... Resources Resources on the Addgene blog Learn about career planning resources What Do I Do Now? Academic...

...overhangs depending upon whether they are on the 3’ or 5’ end of the gene of interest allowing for insertion...of DNA that can move around in a genome. In a laboratory setting, transposons can be used to both introduce...

...single fluorescent biosensor." Scientific reports 5 (2015): 12397. PubMed PMID: 26215030. PubMed Central...measurement and it provides limited information about what materials or organelles are being targeted ...

...Netherlands. Transformation of HEK cells with adenovirus 5 DNA was postdoc Frank Graham’s 293rd experiment, hence... currently assumed that the cells came from an aborted fetus. HEKs have an unusual ploidy and are considered...