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Showing: 1 - 20 of 420 results

  1. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...(A & B) Knockout Mouse X.S. Liu 3rd 5 5 10 49,252 Human Metabolic Gene CRISPRa sgRNA Library 187080 Activation...Weissman 3rd 5 10 104,540 209,080 CRISPRa-v2 83996 1000000093 Activation Mouse Weissman 3rd 5 10 107,105...Weissman 3rd 5 10 104,535 209,070 CRISPRi-v2 83987 1000000092 Inhibition Mouse Weissman 3rd 5 10 107,415...Weissman 3rd 5 Varies hCRISPRi-v2 subpooled libraries 83971 — 83977 Inhibition Human Weissman 3rd 5 Varies ...Weissman 3rd 5 Varies mCRISPRi-v2 subpooled libraries 83989 — 83995 Inhibition Mouse Weissman 3rd 5 Varies ...Mouse Yusa 3rd 5 87,897 Mouse improved genome-wide library v2 67988 Knockout Mouse Yusa 3rd 5 90,230 Mouse...

  2. Chemogenetics AAV Preps

    Type
    Collection
    ...Cre-dependent 5, 9 Sternson 119742 AAV SYN PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Sternson...IRES EGFP none 5 Sternson 121538 pAAV SYN1 HA-hM4D(Gi) hM4D(Gi) - Inhibition HA fusion none 5 Richie 121539...mCitrine none 5 Roth 50478 pAAV-GFAP-hM3D(Gq)-mCherry hM3D(Gq) - Activation mCherry fusion none 5 Roth 50479...) - Activation mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Roth 44362 pAAV-hSyn-DIO-hM4D(Gi...) - Inhibition mCherry fusion Cre-dependent 1, 2, 5, 8, 9, rg*, PHPeB Roth 65417 pAAV-hSyn-dF-HA-KORD-...mCherry hM3D(Gq) - Activation mCherry fusion none 2, 5, 8, 9, rg* Roth 50475 pAAV-hSyn-hM4D(Gi)-mCherry hM4D... hM4D(Gi) - Inhibition mCherry fusion none 2, 5, 8, 9, rg* Roth 154867 pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA...

  3. Optogenetics AAV Preps

    Type
    Collection
    ... 1, 2, 5, 9 Deisseroth 26973 pAAV-hSyn-hChR2(H134R)-EYFP Syn ChR2/H134R EYFP Constitutive 1, 5, 9, rg*...Constitutive 1, 5, rg* Boyden 59171 pAAV-Syn-ChrimsonR-tdT Syn ChrimsonR tdTomato Constitutive 1, 5, 9 Boyden... dependent 5 Boyden 99039 pAAV-CamKII-ArchT-GFP (PV2527) CaMKII ArchT GFP Constitutive 1, 5, 9, rg* Boyden...Constitutive 5, 8, rg* Boyden 84445 pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] CAG Jaws GFP Cre dependent 1, 5, rg* ...dependent 1, 5 Yizhar Inhibitory: PPO 139504 pAAV-CAG-DIO-PPO-Venus CAG PPO Venus Cre dependent 5 Bruchas ... 1, 5 Yizhar 198516 pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE EF1a PdCO mScarlet Cre dependent 1, 5 Yizhar...mCherry-WPRE-HGHpA EF1a ChR2/H134R mCherry Cre dependent 1, 5, 8, 9, rg* Deisseroth 20298 pAAV-EF1a-double floxed-hChR2...

  4. Fluorescent Protein Guide: FRET

    Type
    Collection
    ... four Venus fluorescent proteins connected via a 5, 5 and 6 amino acid linker, respectively VVVVV Anisotropy...Plasmid FRET Pair C5V Cerulean attached to Venus via a 5 amino acid linker C17V Cerulean attached to Venus ... acid linker C5A Cerulean attached to Amber via a 5 amino acid linker C17A Cerulean attached to Amber ...of two Venus fluorescent proteins connected via a 5 amino acid linker V17V Anisotropy/brightness standard... three Venus fluorescent proteins connected via a 5 and 6 amino acid linker, respectively VVVV Anisotropy... of five Venus fluorescent proteins connected via 5 amino acid linkers VVVVVV Anisotropy/brightness standard...consisting of six Venus fluorescent proteins connected via 5 amino acid linkers pET28CLY1 Peptide linker standard...

  5. Immunology Research Plasmids and Resources

    Type
    Collection
    ... HTR3A 5-hydroxytryptamine (serotonin) receptor 3A 5-HT-3, 5-HT3A, 5-HT3R, 5HT3R, HTR3 HTR3B 5-hydroxytryptamine...heavy diversity 5-24 (non-functional) IGHD524 IGHD5-5 immunoglobulin heavy diversity 5-5 DK4, IGHD55 IGHD6...:14099, K5-5, MGC88293 CCR5 chemokine (C-C motif) receptor 5 CC-CKR-5, CCCKR5, CD195, CKR-5, CKR5, CMKBR5...receptor 3B 5-HT3B HTR3C 5-hydroxytryptamine (serotonin) receptor 3, family member C - HTR3D 5-hydroxytryptamine... interleukin 5 (colony-stimulating factor, eosinophil) EDF, IL-5, TRF IL5RA interleukin 5 receptor, alpha...

  6. Luciferase Plasmids

    Type
    Collection
    ...Insertion of 5' promoter/enhancer regions Joshua Mendell 60323 pGL4.23-GW Firefly Insertion of 5' promoter...Description PI 16539 pBV-Luc Firefly Insertion of 5' promoter/enhancer regions Bert Vogelstein 12178 pIS0...normalization William Kaelin 71248 pXPG Firefly Insertion of 5' promoter/enhancer regions. Mutations within the luciferase...luciferase and renilla_polyA Firefly/Renilla Insertion of 5' promoter/enhancer regions upstream of Firefly luciferase.... Mikko Taipale 87789 pPTluc Firefly Insertion of 5' promoter/enchancer regions in Drosopholia Herman ... Calabrese 106253 pLS-mP-Luc Firefly Insertion of 5' promoter/enhancer regions. Lentiviral Nadav Ahituv...Ahituv 106292 pLS-SV40-mP-Rluc Renilla Insertion of 5' promoter/enhancer regions. Lentiviral Nadav Ahituv...

  7. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...parameters: 37 °C for 30 min; 95 °C for 5 min and then ramp down to 25 °C at 5 °C/min. Dilute oligos 1:10 in ddH...following parameters: Cycles 1-20 (37 °C for 5 min, 20 °C for 5 min); Cycle 21 (80 °C for 20 min). These ...enzyme (5,000 U/ml), 5.0 μl ATP (10 mM), 0.25 μl (5 μg) BSA (20 mg/ml), 0.375 μl (750 U) T4 DNA ligase... solution and add to electroporation cuvette. Add 5 μg of each CRISPR/Cas9 construct (10 μg total). Add...construct. Electroporate cells with 250 volts for 5 msec in a 2 mm cuvette using an electroporation system...population exposed to sgRNA-A and sgRNA-B (see step 5 ). The purpose of the following PCR is to validate...multichannel pipette. Centrifuge PCR plate at 400 x g for 5 min and remove supernatant by flicking the PCR plate...

  8. CRISPR Guide

    Type
    Collection
    ...bacterium (LbCpf1) 5' TTTV AsCpf1 RR variant 5' TYCV LbCpf1 RR variant 5' TYCV AsCpf1 RVR variant 5' TATV Campylobacter...seed sequence completely abolish target cleavage, whereas mismatches toward the 5′ end distal to the PAM...sequence may not be characterized *In the table above, 3' and 5' indicate on which end of targeted sequence...will continue to anneal to the target DNA in a 3′ to 5′ direction. Thus, mismatches between the target sequence...application. For example, knockout libraries often target 5′ constitutively expressed exons, but activation and...application. For example, the PAM sequence for SpCas9 (5′-NGG-3′) is abundant throughout the human genome, ...(or Cas9 nickase) Single (or dual) gRNA targeting 5′ exon or essential protein domains High-fidelity Cas...

  9. Neurodegeneration Research Collection

    Type
    Collection
    ...and breathing, and often results in death within 3-5 years from symptom onset. Disease mechanisms are still...having no prior family history. A small percentage (5-10%) are familial ALS cases having at least one other...lentiviral vectors expressing kinesin heavy chain 5 . Zamponi et al. Dev Cell. 2022 Jun 6 Use this avidity-based...mesDA neurons. Maimaitili et al. Nat Commun. 2023 Dec 5. See More Antibodies Find information on Addgene's...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...Scientists are studying protein aggregation, learning about the blood brain barrier integrity, and using CRISPR...with support from the BRAIN Initiative. Jackson Laboratory collection of neurological mouse models including...

  10. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ... actual oligo you should order is: 5'-CACCGN...N-3' 3'-CN...NCAAA-5' You need to add the CACC and AAAC...purification and have tried conditions with 5% DMSO or without 5% DMSO. My genomic PCR didn’t work, do you... http://www.cell.com/abstract/S0092-8674(13)01015-5 As for target selection, we usually pick 3-6 guides... Feng Zhang's Lab, has assembled a list of FAQs about using the lab's CRISPR technology. Genome..., February 2013 ), has assembled a list of FAQs about using the lab's CRISPR technology. Visit the Zhang...

  11. Nuclear Receptor Signaling Atlas (NURSA) Plasmid Guide: Nuclear Receptors

    Type
    Collection
    ...SF-1, SF1, SPGF8, SRXY3 nuclear receptor subfamily 5, group A, member 1 NR5A2 Plasmids NR5A2, B1F, B1F2...FTZ-F1beta, LRH-1, LRH1, hB1F-2 nuclear receptor subfamily 5, group A, member 2 NR6A1 Plasmids NR6A1, GCNF, GCNF1...nuclear receptor plasmid collection assembled in collaboration with the Nuclear Receptor Signaling Atlas (NURSA...associated with numerous disease pathways. In collaboration with research labs from around the globe, Addgene...

  12. AAVED

    Type
    Collection
    ...Event Details Date: Friday September 21, 2018, 9 AM - 5 PM Location: Addgene, 75 Sidney St, Cambridge, MA,...00 PM Wrap Up & Next Steps Melina Fan/Leila Haery 5:00 PM Adjourn Meeting Preparation For Speakers: Each...Learn about Addgene's 2018 AAV Education and Development (AAVED) workshop series. The... Institute Katherine Matho Cold Spring Harbor Laboratory Gowan Tervo Janelia Research Campus Sarada Viswanathan...Institute Katherine Matho, Cold Spring Harbor Laboratory Gowan Tervo and Sarada Viswanathan, Janelia Research...

  13. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Cell Chem Biol. 2016 Jun 1. pii: S2451-9456(16)30163-5. Teresa Alonso , Javier García-Sancho Calcium Large... calmodulin-peptide pairs. Chem Biol. 2006 May;13(5):521-30. Amy Palmer , Roger Tsien Calcium Turquoise... in the endoplasmic reticulum. Biophys Rep (2019) 5, 31–42 Pingyong Xu Magnesium Fluorescent Mg2+ sensor...influx/kynurenine efflux cycle. PLoS Biol. 2007 Oct;5(10):e257. Wolf Frommer Return to Top Neurotransmitters...2023.05.27.542566. Yulong Li Serotonin Serotonin (5-HT) sensor GRAB_5-HT1.0 for viral expression in neurons...measuring serotonin dynamics. Nat Neurosci. 2021 Apr 5. Yulong Li , J. Julius Zhu Serotonin Serotonin sensor...S0092-8674(20)31612-3. Lin Tian Serotonin Serotonin (5-HT) sensor psychLight Psychedelic-inspired drug discovery...

  14. Ras Pathway

    Type
    Collection
    ...Kyprianou K. World J Gastrointest Oncol. 2013 May 15; 5(5): 97–101. doi: 10.4251/wjgo.v5.i5.97. PubMed PMID... Frederick National Laboratory for Cancer Research . Frederick National Laboratory for Cancer Research...

  15. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ...guide RNA ( gRNA ) that uses 20 variable nts at its 5’ end to base pair with a target genomic DNA sequence...construct gRNA expression vectors. More information about RGNs and CRISPR-Cas systems can also be found at...

  16. AAV Viral Preps

    Type
    Collection
    ... vectors for trial purposes in serotypes AAV1, 2, 5, 8, 9 and retrograde Engineered Serotypes Retrograde...are reported with each shipment. For more details about our viral service, visit our Viral Service page ...items are listed on the Addgene inventory pages above (By Function or By Serotype). Convert a Penn Catalog...

  17. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...20R] SUR2B Rat Mouse IgG2a 114481 Anti-QKI-5 [N195A/16R] QKI-5 Human Mouse IgG2a 114482 Anti-Synaptotagmin... IgG2a 114563 Anti-TRIP8b (exon 1a/5) [N291C/22R] TRIP8b (exon 1a/5) Mouse Mouse IgG2a 128612 Anti-Kv2.1... IgG2a 177570 Anti-mGluR1/5 (Group I) glutamate receptor [N75/33R] mGluR1/5 (Group I) glutamate receptor...Mouse IgG2a 182119 Anti-mGluR1/5 (Group I) glutamate receptor [N75/3R] mGluR1/5 (Group I) glutamate receptor...Mouse IgG2a 206550 Anti-Synaptotagmin-5 [N274/8R] Synaptotagmin-5 Mouse Mouse IgG2a 206552 Anti-Histone...190517 Kv1.4 K+ channel (extracellular) scFv [L71/5] L71/5 scFv Kv1.4 K+ channel (extracellular) Rat Mouse...190533 Pan-Gamma-protocadherin-Constant scFv [N159/5] N159/5 scFv Pan-Gamma-protocadherin-Constant Mouse Mouse...

  18. Brain Initiative Collection

    Type
    Collection
    ...Fishell 83896-AAV1 pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression in forebrain...Fishell 83896-AAV9 pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression in forebrain...Fishell 83896-AAVrg pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression in forebrain... 140552-AAV9 pAAV-hsyn-GRAB_5-HT1.0 Expresses the 5-HT sensor GRAB_5-HT1.0 in neurons Yulong Li 140553...particles that are suitable for in vivo use. Learn more about our viral service ID Viral Prep Description PI 65417...well as by our trusted partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity...

  19. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...iSPI_pSer_Subpool#4 Pooled Library 188530 iSPI_pSer_Subpool#5 Pooled Library 188531 iSPI_pSer_Subpool#6 Pooled Library...Nov;19(11):1371-1375. doi: 10.1038/s41592-022-01638-5. PubMed rEcoli XpS and a new pSerOTS Described in:...This library is anticipated to be useful for laboratories performing phosphoproteomic experiments in need...

  20. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...Pool ENgineering (OPEN) method. This kit consists of 5 individually packaged plasmids and 3 individually ...Dove et al., Nature 1997 ) and later modified by Pabo and colleagues ( Joung et al., PNAS 2000 ). The ...
Showing: 1 - 20 of 420 results