We narrowed to 608 results for: Sis

1. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

   Type

   Blog Post

   Published

   Aug. 13, 2014, 3:49 p.m.

   ...nature, with the ultimate goal to improve the diagnosis of disease, and cancer in particular. They recently...

2. 9 Tips to Achieve Success in Academia

   Type

   Blog Post

   Published

   April 1, 2014, 5:33 p.m.

   ...aware of careers outside of academia. How do PIs assist their mentees who have goals outside of the university...

3. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

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   Blog Post

   Published

   March 15, 2018, 12:59 p.m.

   ...configuration. In addition, D10A editing efficiency is consistently higher than that of H840A, especially with smaller...

4. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

   Type

   Blog Post

   Published

   Feb. 1, 2018, 3:07 p.m.

   ...through our Snapgene powered maps and sequence analysis tools.     The main ways we use SnapGene for...

5. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

   Type

   Blog Post

   Published

   Dec. 13, 2017, 3:20 p.m.

   ...Osuna collated Zika case studies, making a meta analysis available to the community on ReFigure. ReFigure...

6. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

   Type

   Blog Post

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   June 5, 2017, 3:19 p.m.

   ... provides unmatched customer service that is consistently timely, informative, friendly, and professional...

7. Bricking Science: Portraying Scientific Reality Through LEGO

   Type

   Blog Post

   Published

   Nov. 29, 2016, 3:30 p.m.

   ...is the most approachable for LEGOs. Stop motion consists of taking many pictures, one for each position...

8. What's the Best Way to Elute and Store Your Plasmid DNA?

   Type

   Blog Post

   Published

   June 4, 2020, 1:22 p.m.

   ...mechanism called depurination. In depurination, the hydrolysis of the glycosidic bond leads to the release of...

9. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

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   Blog Post

   Published

   May 21, 2020, 1:15 p.m.

   ...2011) Power tools for gene expression and clonal analysis in Drosophila. Nature Methods 9:47–55 . https:...

10. Hot Plasmids and Viral Preps - July 2021

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    Blog Post

    Published

    July 27, 2021, 1:15 p.m.

    ...investigating genetic mechanisms related to morphogenesis, cell fate specification, and human disease....

11. CRISPR 101: Drug discovery and CRISPR

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    Blog Post

    Published

    May 4, 2023, 1 p.m.

    ..., fast, and easy. In cases where the mutational basis of a disease is known – repeat expansion, gene knock...

12. Cellosaurus: A Cell Line Information Database

    Type

    Blog Post

    Published

    Feb. 21, 2023, 2:15 p.m.

    ...ever been the target of an ‘-omics’ study or is resistant to specific compounds, links to publication references...

13. Hot Plasmids - October 2022

    Type

    Blog Post

    Published

    Oct. 6, 2022, 1:15 p.m.

    ...high-throughput screening platform to apply saturating mutagenesis on 21 positions in the protein sequence of the...

14. Understanding GPCR Signaling Using TRUPATH

    Type

    Blog Post

    Published

    Dec. 14, 2020, 2:15 p.m.

    ...(Olsen RHJ et al., 2020). A transducer complex consists of a trimer of three subunits: alpha, beta, and...

15. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type

    Blog Post

    Published

    Jan. 21, 2020, 2:30 p.m.

    ...for the user. This approach serves to maintain consistency with published screen interpretations and allows...

16. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type

    Blog Post

    Published

    Oct. 18, 2017, 1 p.m.

    ...from home and looking for a job.” But Caitlin’s persistence paid off: “I spent about 6 months just editing...

17. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type

    Blog Post

    Published

    Oct. 3, 2017, 12:11 p.m.

    ...performance data, protocols, and even certificates of analysis.” Han’s biggest piece of advice for those looking...

18. Running for Rare Disease, Running for FOP, Running for AJ

    Type

    Blog Post

    Published

    Sept. 15, 2016, 2:30 p.m.

    ...put on typical middle age weight and “exercise” consisted of taking walks in the mall. I took action, joined...

19. Management for Scientists: Giving Feedback

    Type

    Blog Post

    Published

    Feb. 6, 2015, 2:46 p.m.

    ...Feedback is help and it should result in authentic assistance for the recipient to become more effective and...

20. Choosing Your Perfect Empty Backbone

    Type

    Blog Post

    Published

    Aug. 19, 2014, 3:39 p.m.

    ...with the proper selection marker or antibiotic resistance. Next, consider the expression level of your ...

21. Advice for Choosing a Research Project

    Type

    Blog Post

    Published

    April 17, 2014, 2:45 p.m.

    ...to use to reach these milestones? Can you get assistance from people who have the required expertise to...

22. The Stingy Scientist: How the Baby Gel Box Was Born

    Type

    Blog Post

    Published

    April 15, 2014, 3:11 p.m.

    ...before the advent of techniques like PCR and DNA synthesis. Most molecular biologists bought the pricey stuff...

23. CRISPR/Cas9 FAQs Answered!

    Type

    Blog Post

    Published

    March 13, 2014, 4:08 p.m.

    ... cells (for example, plasmid with a puromycin resistance cassette, such as PX459). After you co-transfect...

24. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type

    Blog Post

    Published

    June 27, 2018, 1:16 p.m.

    ...fluorophore, or APEX2), allowing for quantitative analysis using different detection methods. Since the anti-GFP...

25. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type

    Blog Post

    Published

    Oct. 10, 2019, 12:41 p.m.

    ...Central PMCID: PMC427010. Porter, R. (1959). The hydrolysis of rabbit y-globulin and antibodies with crystalline...

26. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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    Blog Post

    Published

    June 2, 2020, 1:15 p.m.

    ...designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized GFP variant...

27. Hot Plasmids and Viral Preps - September 2021

    Type

    Blog Post

    Published

    Sept. 21, 2021, 1:15 p.m.

    ...selection marker, Thy1.1, in addition to puromycin resistance.  The MYC-CRISPR library targets E-boxes genome-wide...

28. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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    Blog Post

    Published

    June 22, 2021, 1:15 p.m.

    ...The lab saw that the B.1.351 variant was very resistant to vaccine serum responses. This result was similar...

29. Viral Vectors 101: Transductions

    Type

    Blog Post

    Published

    April 30, 2024, 1:15 p.m.

    ...non-integrating. Read on to find out more! Molecular basis of transduction Transductions utilize the ‘normal...

30. What the HEK?

    Type

    Blog Post

    Published

    April 18, 2023, 1:15 p.m.

    ...many immature neuron-like qualities, which is inconsistent with kidney cells (Shaw et al.). Since then,...

31. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type

    Blog Post

    Published

    May 25, 2021, 2:29 p.m.

    ...neuroscience research. More broadly, advances in gene synthesis, affinity reagent screening, and next-generation...

32. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type

    Blog Post

    Published

    Sept. 15, 2015, 2:30 p.m.

    ...AHC suffer from recurrent attacks of unilateral paralysis (hemiplegia) that are often painful and accompanied...

33. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type

    Blog Post

    Published

    July 7, 2015, 3:36 p.m.

    ...selection cassette containing genes for hygromycin resistance and the Roller movement phenotype. To make these...

34. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type

    Blog Post

    Published

    April 7, 2015, 4:21 p.m.

    ...control The CRISPR interference (CRISPRi) model consists of a catalytically dead Cas9 protein (dCas9) that...

35. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type

    Blog Post

    Published

    March 4, 2014, 4:49 p.m.

    ...became aggressive. If we can understand the neural basis of violence or aggression, it might do a lot to ...

36. FPbase: A new community-editable fluorescent protein database

    Type

    Blog Post

    Published

    May 16, 2018, 1 p.m.

    ...lineages, 2-photon spectra, more elaborate spectrum analysis tools, and a better way to store and represent...

37. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type

    Blog Post

    Published

    Sept. 19, 2017, 1:20 p.m.

    ...., Vitriol, E., & Hahn, K. (2008). Chromophore-assisted laser inactivation in cell biology. Trends in ...

38. Pushing the Limits of DNA Assembly

    Type

    Blog Post

    Published

    Sept. 6, 2017, 1:01 p.m.

    ...HiFi can simplify multi-position site-directed mutagenesis by assembling multiple fragments containing the...

39. Visualizing Translation at the Single Molecule Level

    Type

    Blog Post

    Published

    Aug. 1, 2017, 1:15 p.m.

    ...References 1. Ingolia, Nicholas T., et al. “Genome-wide analysis in vivo of translation with nucleotide resolution...

40. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type

    Blog Post

    Published

    July 27, 2017, 1:11 p.m.

    ...sample. The second sensor is a fusion protein consisting of sfGFP fused to a His-tagged ABC transporter...