We narrowed to 602 results for: ACE

1. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   Blog Post

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   April 19, 2016, 2:30 p.m.

   ... is sedimentation equilibrium analytical ultracentrifugation [2]. Some affinity assays used in the papers...

2. Light Sheet Fluorescence Microscopy

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   July 16, 2015, 2:30 p.m.

   ...Protocols, 7(11), 1983–1995. Pubmed. K. Chung, J. Wallace, S.Y. Kim, S. Kalyanasundaram, A.S. Andalman, T.J...

3. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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   May 22, 2015, 4:58 p.m.

   ...Insect Populations: I. Cage Studies of Chromosome Replacement by Compound Autosomes in Drosophila melanogaster...

4. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   Blog Post

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   Dec. 12, 2013, 3:35 p.m.

   ...plugging into the probiotic and cellular therapeutic space. If you were to look forward to the future of the...

5. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   Blog Post

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   Oct. 10, 2023, 1 p.m.

   ...offers is marginal. Fortunately, amp can easily be replaced with carb if satellite colonies are an issue. ...

6. Course-Based Undergraduate Research Experiences (CUREs)

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   Blog Post

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   Feb. 9, 2023, 2:15 p.m.

   ....     Fig. 2: Students in an MDH CURE replaced select residues of human mitochondrial MDH (hMDH2...

7. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   Blog Post

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   Sept. 6, 2018, 12:02 p.m.

   ... a Cas9 mutant (Cas9M1C/C80S) that harbors two surface exposed cysteines, including the native C547, allowing...

8. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   Blog Post

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   Aug. 27, 2020, 1:15 p.m.

   ...of your PIPs in which the protein-interaction interface has been compromised (Kodama and Hu, 2012). Such...

9. Transferable Skills Guide: Identifying Your Transferable Skills

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   Blog Post

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   Jan. 11, 2018, 3:29 p.m.

   ...that you can bring into pretty much any other workplace. To get your mind working, take a look at the ...

10. MXS Chaining

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    Feb. 7, 2017, 3:30 p.m.

    ...assembled next to each other, a third module cannot be placed between them, but rather, can only be added to ...

11. Negative Can Be Positive: Open AAV Data with Addgene

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    Blog Post

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    Sept. 6, 2022, 1:15 p.m.

    ... or if and how much the spread of the virus to adjacent areas impacted the experiments we wanted to perform...

12. Lentiviral Vector Uses and Overview

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    Blog Post

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    May 19, 2016, 2:30 p.m.

    ...Galacteros F, Beuzard Y, Gluckman E, Bushman F, Hacein-Bey-Abina S, Leboulch P. Nature. 2010 Sep 16;467...

13. Plasmids 101: Colony PCR

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    Blog Post

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    May 12, 2016, 2:30 p.m.

    ...verify if the colony PCR primers work in the first place: the backbone vector with and without an insert....

14. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    Blog Post

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    March 17, 2016, 2:30 p.m.

    ...transcription (Figure 1). All viral genes are removed and replaced by a single (non-viral) reporter gene (e.g. firefly...

15. Tips for Screening with Yeast Two Hybrid Systems

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    Blog Post

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    Oct. 22, 2015, 2:30 p.m.

    ...yeast! Many false negatives stem from or are exacerbated by expression of target proteins in heterologous...

16. Cpf1: A New Tool for CRISPR Genome Editing

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    Blog Post

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    Oct. 14, 2015, 2:30 p.m.

    ...Cas9’s, but with the direct repeat preceding the spacer rather than following it. The Cpf1 crRNA is also...

17. A Career in Grants: How To Become a Grant Professional

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    Blog Post

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    Aug. 20, 2015, 2:30 p.m.

    ...different issues, and our program didn’t stay in one place within the city infrastructure. My career path changed...

18. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    Blog Post

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    Dec. 2, 2014, 7:23 p.m.

    ...'ve found that research institutes in different places have very different cultures. In Basel, I felt ...

19. Your Lentiviral Plasmid FAQs Answered

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    Blog Post

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    April 23, 2014, 1:08 p.m.

    ...expression of not only the transgene but also that of adjacent cellular genes. Q7: Which genes are deleted or...

20. Negotiating Work and Life: How to Find the Joy

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    Blog Post

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    March 20, 2014, 3:04 p.m.

    ...“in the zone” and everything seems to fall into place. When the balance tips, we need to look to for support...

21. Plasmids 101: Shuttle Vectors

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    Blog Post

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    May 20, 2025, 1:15 p.m.

    ...functional in the organism your experiment takes place in. In many cases, you won’t need to worry about...

22. Twenty Years of Sharing: Expanding Accessibility

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    Blog Post

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    Nov. 21, 2024, 10:47 p.m.

    ...Beijing Zhongyuan 2012 China Summit Pharmaceuticals International 2013 Japan Leehyo Bioscience...

23. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    Blog Post

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    Sept. 13, 2018, 1:55 p.m.

    ...all genes involved in the production of a cell surface glycan that’s required for ricin uptake. The ricin...

24. Transferable Skills Guide: External Collaborations

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    Blog Post

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    April 19, 2018, 1:25 p.m.

    ...social media is a connector and virtual meeting place: You can follow interesting people on twitter and...

25. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post

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    July 2, 2019, 12:51 p.m.

    ...researchers inserted human CD8, a gene that encodes a surface protein for which there are good flow cytometric...

26. Designing Your Chalk Talk for the Academic Job Interview

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    Blog Post

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    March 12, 2019, 1:37 p.m.

    ...talk?" So what exactly is a chalk talk? On the surface, a chalk talk is quite simple. It's you, a dry ...

27. An Inside Look into Addgene's Dev Team

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    Blog Post

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    Jan. 23, 2020, 2:45 p.m.

    ...might be calling something a sample and in other places, we might be calling it an aliquot. This can lead...

28. Overcoming the AAV Size Limitation for CRISPR Delivery

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    Blog Post

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    Sept. 16, 2020, 1 p.m.

    ... REPAIR (RNA Editing for Programmable A to I Replacement), system is also small enough to deliver with...

29. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post

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    Nov. 1, 2018, 12:35 p.m.

    ...Successful cloning occurs when the ccdB gene is replaced by the gene of interest. As a result, cells that...

30. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    Blog Post

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    Dec. 14, 2017, 2:08 p.m.

    ... 21243013. PubMed Central PMCID: PMC3193421. 4. Hacein-Bey-Abina, S., et al., LMO2-associated clonal T...

31. Mesothelioma - Causes, Symptoms, and Treatment

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    Blog Post

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    Dec. 13, 2016, 3:30 p.m.

    ...Addgene's Cancer Collection Pages Learn How the ClonTracer Library Can be Used to Study Cancer Drug Resistance...

32. Antibiotic Resistance: An Old Solution but a New Problem

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    Blog Post

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    Nov. 10, 2016, 3:30 p.m.

    ...is cleaning and disinfecting frequently touched surfaces like tabletops, toys, gym equipment, etc. Virtually...

33. Changing Labor Laws Bring Increased Postdoc Wages

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    Blog Post

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    Oct. 25, 2016, 2:30 p.m.

    ... yet announced what they are doing, however, may face greater challenges. Current salary information that...

34. Antibodies 101: Normalization and Loading Controls for Western Blots

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    Blog Post

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    April 8, 2025, 1:15 p.m.

    ...choose, your loading control measurement will take place either before or after blotting for your POI. ...

35. Adeno-associated Viruses (AAVs) for Genome Editing

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    Blog Post

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    March 27, 2018, 1:32 p.m.

    ...the viral vector. This delivery method frees up space in the AAV allowing a researcher to fill it with...

36. Antibodies 101: Introduction to Immunofluorescence

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    Blog Post

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    Nov. 16, 2021, 2:15 p.m.

    ...this commentary by Uhlen, et al. 2016 is a good place to start.) Reviewing available antibodies can seem...

37. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

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    Blog Post

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    April 9, 2019, 1:13 p.m.

    ...several ways to measure stickiness. In vitro, ultracentrifugation or gel filtration is the most common analytical...

38. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    Blog Post

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    March 5, 2019, 1:08 p.m.

    ...brightness of Tetbow allows this system to be used to trace neurons over millimeter-long distances across multiple...

39. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    Blog Post

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    Feb. 22, 2017, 3:30 p.m.

    ...Pooled Libraries to Generate Mutant Populations Use REPLACER Mutagenesis to Mutate Your Plasmids Resources...

40. 10 Steps to a Perfect Science Talk

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    Blog Post

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    Aug. 23, 2016, 2:30 p.m.

    ...Joanne's "How to Choose the Right Next Lab or Workplace" On YouTube Joanne's 10 Things Scientists Ask ...

41. Using Phosphoserine to Study Protein Phosphorylation

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    Blog Post

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    June 23, 2016, 2:30 p.m.

    ...expression vectors, the codon for the amino acid to be replaced with phosphoserine must be recoded to ‘TGA’ using...

42. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    Blog Post

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    Jan. 16, 2025, 3:42 p.m.

    ...variables, we suggest these serotypes as a good place to start. Table 2: AAV tissue tropism for mice in...

43. CRISPR 101: Off-Target Effects

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    Blog Post

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    Feb. 8, 2024, 2:15 p.m.

    ...experiments, there is hardly any flexibility in gRNA placement (e.g., mutating a specific amino acid or introducing...

44. A Control for All Seasons

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    Blog Post

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    Oct. 5, 2023, 1:15 p.m.

    ...2018). In this control, the primary antibody is replaced with pre-immune serum from the animal the antibody...

45. How to Design Your gRNA for CRISPR Genome Editing

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    Blog Post

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    Sept. 24, 2020, 1:15 p.m.

    ...optimized sequence will do little if it is in the wrong place, but because the target window is more-narrow, there...

46. Finding Your Perfect Job After University

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    Blog Post

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    Jan. 12, 2016, 3:30 p.m.

    ...developing tropical country. This is why I set up a placement working in a rural health clinic in Ghana. My ...

47. Deep Dive: Statistical Tests (Comparisons)

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    Blog Post

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    March 7, 2023, 2:15 p.m.

    ...Douglas. Explorations in statistics: statistical facets of reproducibility. Adv Physiol Educ. 40(2): 248...

48. Important Considerations When Using AAVs

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    Blog Post

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    June 13, 2017, 2:30 p.m.

    ...bleach solution should be used to clean AAVs from workspaces. Speed: AAV transduction experiments are ...

49. Sequencing Options for CRISPR Genotyping

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    Blog Post

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    Oct. 4, 2016, 2:30 p.m.

    ...-target breaks Prediction algorithms: A good place to start for biased validation At the moment, many...

50. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    Blog Post

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    April 25, 2017, 2:30 p.m.

    ...continuous excitation. By generating PA-FPs fused to intracellular proteins, scientists can track single protein...