We narrowed to 609 results for: CaS;
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TypeBlog PostPublishedJune 22, 2017, 2:30 p.m....targeted to the marker proteins, however, in this case the cell needs to be fixed and the cell membrane...
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PhD Applications After COVID
TypeBlog PostPublishedJan. 5, 2023, 2:15 p.m....you intend to work with, so reach out to three in case one isn’t able to respond to your email. During ... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....such as TagRFP. Problem 3 can be solved, in most cases, by using monomeric FPs or FPs from different families... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....air bubbles or high concentration of fluorophores) cast shadows along the illumination path. The shadows... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m....directly into your preferred buffer. If that’s the case, a buffer exchange can be performed using a desalting... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m.... blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24 for simplifying plant genetic engineering... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog PostPublishedFeb. 27, 2018, 2:04 p.m....antibody (HCab), which are unique to camels, llamas, alpacas and other camelids. In terms of structure, HCabs... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog PostPublishedSept. 8, 2016, 2:30 p.m....fluorescence upon binding to its cognate protein. In some cases this property eliminates the need for removing excess... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog PostPublishedAug. 9, 2016, 2:30 p.m....regions and their reverse complements were parsed for spCas9 PAM sites (NGG) and then filtered based on two ... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m....Screening Read about Genome-Wide Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out ... -
AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m.... of new capsid variants, designing expression cassettes to regulate and optimize transgene levels, and... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m.... infect the cell type of interest, which, in the case of Chan et al, was neurons and astrocytes. Why ... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m....binding by tweaking those parameters, but in some cases your antibody may just not be suitable for use with... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m....impacts the success of BiFC experiments. In either case, overexpression should be avoided because it can... -
Plasmids 101: Protein tags
TypeBlog PostPublishedDec. 11, 2014, 4:26 p.m.... blots, IP, co-IP, IF, flow -cytometry; can occassionally interfere with protein folding HBH HHHHHHAGKA... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m.... series of retroviral vectors expressing tagged dCas9 and guide RNA. The retroviral enChIP system enables... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog PostPublishedMay 15, 2014, 3:33 p.m....used to study protein-protein interactions. In this case, two portions of EGFP are fused to the proteins ... -
Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)
TypeBlog PostPublishedJuly 8, 2025, 1:15 p.m.... will save you a lot of headaches down the line! Cash in on gene synthesis Before you start your cloning... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog PostPublishedNov. 21, 2024, 10:47 p.m....Sinapse Biotecnologia 2022 Brazil In some cases, the distributors broadly helped increase access... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog PostPublishedApril 2, 2019, 1:44 p.m....both homeobox genes that are part of a genetic cascade that determines the cell fate of the dorsal mesoderm... -
Lab to Office Culture Shock
TypeBlog PostPublishedFeb. 5, 2019, 1:04 p.m....inevitably have downtime and plenty of it. That’s not the case at an office job. I’ve frequently told job candidates... -
Antibodies 101: Reading a Flow Plot
TypeBlog PostPublishedFeb. 6, 2024, 3:13 p.m.... by looking at the different populations. In the case of Figure 2, one population is B- and another two... -
Hot Plasmids - November 2023
TypeBlog PostPublishedNov. 14, 2023, 3:35 a.m....edits, such as long or highly structured templates. Cas9 variants PE6e–g often had even higher editing efficiencies... -
Viral Production at Addgene
TypeBlog PostPublishedOct. 24, 2023, 1:15 p.m.... contamination. To date, Addgene has never had a case of mycoplasma contamination. In the event of contamination... -
How to Write a Scientific Review Article
TypeBlog PostPublishedFeb. 16, 2017, 3:30 p.m....Finally, you can sound credible and cool when you casually mention “Haery et al., showed that MYC expression... -
Plasmids 101: Gateway Cloning
TypeBlog PostPublishedJan. 12, 2017, 3:30 p.m....product or plasmid with an attP donor vector. In this case, we would use PCR to add attB sites to either end... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog PostPublishedNov. 10, 2016, 3:30 p.m....even make their own antibiotic compounds. In such a case, the microbe itself would be unharmed by the compound... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog PostPublishedDec. 14, 2017, 2:08 p.m....per 100 ml of starting blood sample, in the best-case scenario [7]. In the clinical setting where patient... -
Transferable Skills Guide: Career Planning Resources
TypeBlog PostPublishedNov. 14, 2017, 2 p.m....outside of the box (most likely your lab, in this case). It is important to realize that you have to take... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog PostPublishedOct. 25, 2016, 2:30 p.m....the salary changes expected for postdocs in many cases should be as described in the notice of the new ... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog PostPublishedJuly 25, 2024, 1:15 p.m....process can be laborious and expensive. In some cases, conjugation methods can interfere with binding,... -
Tips for Writing a Good Cover Letter
TypeBlog PostPublishedMay 21, 2024, 1:30 p.m.... to three paragraphs. This is where you make the case for why you are a good candidate, explain how your... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog PostPublishedApril 11, 2024, 1:15 p.m....incorporate interchangeable antibiotic resistance cassettes (Prior et al. 2010), inducible promoters with ... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog PostPublishedSept. 17, 2019, 12:40 p.m....harbouring a Cre-dependent IL-1 beta overexpression cassette. Expression of Cre in these neurons turns on overexpression... -
Bioinformatics at Addgene
TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m....Nextflow for bioinformatics pipelining In many use-cases, including ours at Addgene, the overall purpose ... -
Getting the Most from Your Lentiviral Transduction
TypeBlog PostPublishedApril 7, 2016, 2:30 p.m....in a higher volume of lower titer virus. In such cases you may want to consider concentrating your viral... -
Tips for Using FRET in Your Experiments
TypeBlog PostPublishedNov. 5, 2014, 3:41 p.m....with the laser used to excite the donor. In that case the cross-talk is high and your background signal... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog PostPublishedApril 3, 2014, 8:05 p.m....oftentimes put to therapeutic uses, and in those cases it's important to identify the right tissue-specific... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog PostPublishedMarch 5, 2019, 1:08 p.m.... same XFP transgene in an adult fly. As a stop cassette is located directly upstream of the fluorescent... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog PostPublishedJan. 29, 2019, 2:27 p.m....cellular RNA to specific intracellular locations using Cas9 and track the localization of the complex. Broccoli... -
March for Science
TypeBlog PostPublishedApril 21, 2017, 2:30 p.m....Information on how to contact your representatives in case you would like to talk with them about science: ... -
Evolution of Lab Techniques
TypeBlog PostPublishedJune 21, 2016, 2:30 p.m....detection methods are also commonly used. In all cases, the result can be quantified by the strength of... -
An Integrin Antibody Toolkit from IPI
TypeBlog PostPublishedMarch 28, 2024, 1:15 p.m....and migration, initiating intracellular signal cascades, and allowing agile responses to mechanical forces... -
Transferable Skills Guide: Conflict Resolution
TypeBlog PostPublishedJan. 4, 2022, 2:15 p.m....perceive any conflict as inherently bad, and in many cases it can be. If people or teams can’t see eye to eye... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog PostPublishedSept. 1, 2015, 2:30 p.m....that means saying no to a negotiation or, in this case, to academia altogether. The primary point of ... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog PostPublishedSept. 29, 2016, 2:30 p.m....what her cat is thinking. Find her on Twitter @JessicaSacher. References 1. Nobrega, Franklin L., et al... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog PostPublishedOct. 2, 2018, 12:56 p.m....undermining personal integrity, destructive innuendo and sarcasm, persistent teasing, name calling, insults, intimidation... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m....lacking gaps or mismatched base pairs (as in the case for LCR, LDR and Padlock Probes), use of a high ... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog PostPublishedNov. 2, 2021, 1:15 p.m....of AAV vector we produce containing a DIO/FLEx cassette using next-generation sequencing (NGS) (Guerin... -
A Guide to Getting Started in Undergrad Research
TypeBlog PostPublishedJune 8, 2021, 1:15 p.m....think reflecting on why it is we enjoy, or in some cases, don’t enjoy, is worthwhile and can help us make...