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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 612 results for: SCR

Showing: 551 - 600 of 612 results

  7. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ... separately). Figure 5: Mechanism of ATLASCre. VAMP2 = synaptobrevin 2; At = ALFA-tag; BACE ...

  8. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...double-stranded break, while modulators regulate transcription and chimeric efforts make a precise edit or ...

  9. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...proteasome for degradation. Like several other tags described here, the dTAG uses a small molecule, a tag, and...

  10. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...that perfect. One of the known limitations was described by Schüchner, et al., who published a paper showing...

  11. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...lab, research, and provide them with a CV or transcripts if requested Thank them, and eagerly await a ...

  12. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...concentration Low copy plasmids produce fewer transcripts of their antibiotic resistance genes and can ...

  15. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...served. I hope that with some of the information described above you feel empowered to start your own journal...

  18. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ... (and therefore between early and late gene transcription) is demarcated by viral genome replication (...

  19. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...generate transgenic Arabidopsis, from studying transcriptional and translational dynamics of genes and proteins...

  20. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ... minimize false positive colonies. We routinely screen just two colonies for simple cloning procedures...

  21. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...reading up on validating genome edits to find screening strategies for your experiment. Also learn about...

  23. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...examples. The tables list more common tags than are described in the post, but have been categorized to help...

  24. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...our eBook: Plasmids 101 Learn about genome-wide screening with CRISPR and Lentivirus Read about the ClonTracr...

  27. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...primary antibody and serum immunoglobulins indiscriminately, causing competition for binding sites. In...

  28. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...Gateway technology relies on the two reactions described below: The BP Reaction takes place between the...

  33. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...into E. coli harboring the defective CamR gene. Screening for surviving colonies and subsequent rounds of...

  34. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ... able to edit A → Y (Y = T or C) in 67.17% of screening cells (Tong, Wang, et al., 2023). Based on this...

  36. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...free of contamination and match their original description. The oldest, largest, and most diverse of these...

  39. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...gene of interest: Plasmid Color Expression Description pPROEX Aqua Cyan Bacterial Expresses Aquamarine...

  40. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...Xing, X.-H. (2018). Pooled CRISPR interference screening enables genome-scale functional genomics study...

  42. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...freeze all grants, contracts The Guardian: Trump to scrap Nasa climate research in crackdown on “politicized...

  44. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice...

  46. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...feedback. Many of these documents are too vaguely described in other sources of guidance or the document examples...

  47. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...the same). Examples of such targets include transcription factors, histones, and DNA repair enzymes. Note...

  48. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...collection includes 26 unique antibodies that bind discrete subunits or particular subunit pairs. Some of ...

  49. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...listeners to be thinking “What happens next?”  Describe how you pushed to a solution or overcame obstacles...
Showing: 551 - 600 of 612 results