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  1. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...trait to their granddaughters that reduced their lifespan. Because these dramatic conditions were experienced...so the syndromes are caused by a lack of the corresponding gene products due to the same region, but not...

  2. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...have to, but this is tough for women in skirts, especially short people – which I am, so I hate stools)....listing some and asking people to raise hands in response. I am always ready with some written poll questions...

  3. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ... a company. Revolution Bioengineering is two responsible adults in their 30s who have quit their academic...development: building a plant that could sense and respond to novel substances. We both had an applied science...

  4. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...Academic postdocs can be long—5 years or more, especially in the biological sciences—whereas industry postdocs...make that transition from an academic setting—especially if job applications require teaching experience...

  5. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...deployed around the world to combat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus...cell-surface receptor. The spike protein RBD responsible for binding ACE2 is the target of most anti-SARS-CoV...

  6. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...interest to better study them in vivo and in vitro, especially in the mid-late 20th century with the advent ...tumorigenesis.  Using two different synthetic peptides corresponding to linear sequences within the middle of the...

  7. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...during the production process? These questions are especially important when producing multiple vectors in ... with the most total reads are most likely to correspond to the contaminant. Each contig is then BLASTed...

  8. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...complementary and non-complementary to the gRNA, respectively. When complexed with a truncated gRNA, Cas9 ...transcriptional activation or repression at the respective sites.   Many thanks to our guest bloggers Alissa...

  9. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ... agitated with sudden changes in temperature (especially if you have air conditioning on) so leave them...cell line you’re using and its passage number. Especially in a lab where multiple people are working on...

  10. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...so reach out to three in case one isn’t able to respond to your email. During my PhD application cycle,...interview from a travel day to an online call in response to challenges on their end. Though the quick changes...

  11. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    .... In this scenario, gRNAs that are “enriched” correspond to genes that confer drug resistance when mutated...library plus Cas9 to generate the mutant cells, respectively (C). Mutant cells are screened (D) and "hits...

  12. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ... on popular support, which in turn depends on widespread trust in scientists to develop new technologies...trusted to handle such a powerful technology responsibly. An accidental release would be particularly ...

  13. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ... figure organization with a different person responsible for each figure. Others still are more casual...focus and critical mass of participants. This is especially true of journal clubs where the very point is...

  14. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...Infographics Low Combines both words and images; especially useful for conveying and contextualizing data...except for space) Real-time audience engagement and response. High connection levels and interaction. Can bring...

  15. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...human therapeutics (e.g. using Sleeping Beauty). Despite their diverse utility, DNA transposons have been...Ladha A, Makarova KS, Koonin EV, Zhang F (2020) Response to Comment on “RNA-guided DNA insertion with CRISPR-associated...

  16. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...Mikhaylova, et al. 2015).  Despite these limitations, ExM is a valuable tool, especially when paired with complementary...

  17. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...students. But he also found that his teaching responsibilities left him with limited time for one-on-one ...professional risk involved in setting up a CURE, especially if it’s a new approach within their department...

  18. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...you can inhibit cell cycle regulatory proteins responsible for the transition out of S phase, so cells stay...Methods favoring homology-directed repair choice in response to CRISPR/Cas9 Induced-double strand breaks. Int...

  19. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...AsCpf1 and LbCpf1 (1307 and 1228 amino acids long respectively), both cleave in a staggered pattern similar...rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins...

  20. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...neighboring ssRNA and ssDNA by Cas13 and Cas12a, respectively, to cleave and activate a reporter. The detectable...amplification) or reverse transcriptase (RT)-RPA, respectively. RPA is coupled with T7 transcription to convert...
Showing: 221 - 240 of 545 results