We narrowed to 559 results for: ESP

...great tips to guide your experimental design, especially the design of the RNA sequences you’ll use in...increase the odds of incorrect editing outcomes, especially when MMR is inhibited. Created with BioRender.com...

... from the membrane along with the antibodies, especially with more stringent stripping methods. If you...horseradish peroxidase (HRP) or Alexa Fluor dyes, respectively. Chromogenic detection produces colored precipitates...

..., some adenovirus strains are known to cause respiratory diseases in humans, earning this virus a BSL-... and closely follow safety protocols. This is especially true when you’re tired, in a rush, or having ...

...experience helping scientists share plasmids, especially those of the CRISPR variety (see slides from ...conserved in mammals, expressed in neurons, and are especially abundant in neuronal synapses. shRNA silencing...

...trait to their granddaughters that reduced their lifespan. Because these dramatic conditions were experienced...so the syndromes are caused by a lack of the corresponding gene products due to the same region, but not...

...have to, but this is tough for women in skirts, especially short people – which I am, so I hate stools)....listing some and asking people to raise hands in response. I am always ready with some written poll questions...

... a company. Revolution Bioengineering is two responsible adults in their 30s who have quit their academic...development: building a plant that could sense and respond to novel substances. We both had an applied science...

... agitated with sudden changes in temperature (especially if you have air conditioning on) so leave them...cell line you’re using and its passage number. Especially in a lab where multiple people are working on...

...during the production process? These questions are especially important when producing multiple vectors in ... with the most total reads are most likely to correspond to the contaminant. Each contig is then BLASTed...

...complementary and non-complementary to the gRNA, respectively. When complexed with a truncated gRNA, Cas9 ...transcriptional activation or repression at the respective sites.   Many thanks to our guest bloggers Alissa...

...interest to better study them in vivo and in vitro, especially in the mid-late 20th century with the advent ...tumorigenesis.  Using two different synthetic peptides corresponding to linear sequences within the middle of the...

...so reach out to three in case one isn’t able to respond to your email. During my PhD application cycle,...interview from a travel day to an online call in response to challenges on their end. Though the quick changes...

...deployed around the world to combat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus...cell-surface receptor. The spike protein RBD responsible for binding ACE2 is the target of most anti-SARS-CoV...

...Academic postdocs can be long—5 years or more, especially in the biological sciences—whereas industry postdocs...make that transition from an academic setting—especially if job applications require teaching experience...

.... In this scenario, gRNAs that are “enriched” correspond to genes that confer drug resistance when mutated...library plus Cas9 to generate the mutant cells, respectively (C). Mutant cells are screened (D) and "hits...

... on popular support, which in turn depends on widespread trust in scientists to develop new technologies...trusted to handle such a powerful technology responsibly. An accidental release would be particularly ...

...human therapeutics (e.g. using Sleeping Beauty). Despite their diverse utility, DNA transposons have been...Ladha A, Makarova KS, Koonin EV, Zhang F (2020) Response to Comment on “RNA-guided DNA insertion with CRISPR-associated...

...Infographics Low Combines both words and images; especially useful for conveying and contextualizing data...except for space) Real-time audience engagement and response. High connection levels and interaction. Can bring...

...Mikhaylova, et al. 2015).  Despite these limitations, ExM is a valuable tool, especially when paired with complementary...

...students. But he also found that his teaching responsibilities left him with limited time for one-on-one ...professional risk involved in setting up a CURE, especially if it’s a new approach within their department...