We narrowed to 570 results for: FIE

...assignments. The instructors are leaders in the field of microbiology with multiple high-profile publications...should be able to be enjoyed and appreciated by all fields not solely the specific area the writer works.  ...

...community. For our first concrete steps into this field, we’ve curated microbiology plasmids from the repository...network with scientists in this diverse and exciting field and to find out how we can serve them better. Here...

...content coming also keeps me on top of a variety of fields thereby broadening my knowledge. In addition, this...distillation, etc) are transferable to many different fields. If you start thinking about preparing yourself...

...dissociate under blue light. This tool has now been modified by Torsten Wittmann’s lab at UCSF to modulate ...of the LOVTRAP domains. When both halves of the modified EB1 protein are overexpressed in cells, microtubule...

...and PfAgo, another thermophilic Argonaute. They identified NgAgo and showed that it can cleave plasmid and...removing 1-20 nucleotides from the cleavage site specified by the gDNA. Depending on your application, this...

...of the primer that binds to the sequence to be amplified (usually 18-21bp) When selecting restriction... primer that hybridizes to the sequence to be amplified (the ORF in this case), not the Tm of the entire...

... as a seizure disorder.” In 2012, researchers identified the gene responsible for the disease as ATP1A3...specifically, they’ve proposed to use plasmids with modified nickase Cas9 protein in order to target the specific...

...et al. made the surprising discovery that both purified CPP-tagged protein and an untagged control could...subsequently enter the nucleus and cleave the specified target. Figure adapted from D’Astolfo et al. ...

...inhibitors cycloheximide and puromycin, Halstead et al. verified that translation is necessary for removal of GFP...the mCherry labeled mRNA and keeps it in a single field of view for constant tracking. You can then directly...

...  Devarshi Gajjar has 10 years experience in the field of bacterial and fungal pathogenesis. Her research... scientists working in a wide range of research fields and geographical locations. In addition to providing...

...Identifying a host institute or lab So, you’ve identified a gap or need in your research and decided you...of these situations apply to you? Once you’ve identified one or more labs that you would like to visit...

... biological processes is highly useful in other fields, including synthetic biology. Addgene depositors...in optimizing TCSs. Over 75,000 TCSs have been identified in bacteria, and with similar optimization methods...

...today, where he helps researchers in a variety of fields develop RNAi screens to answer their own research...In the case of CRISPR, the hype was completely justified… it has been just an incredibly useful technology...

...student or postdoc. Although the chosen research field is important, the end goal during this time is to...like a scientist. Of course, the specific research field should be interesting; however, this should (in ...

...SLIC cloning process, a gene of interest is PCR amplified to add 5′ and 3′ homology regions from the target...assembly with SLIC. Multiple fragments are PCR amplified to add 5′ and 3′ homology regions to the adjacent...

... aren’t the only investigators in the chromatin field who have deposited with Addgene, but you’ll find...DW Cell. 2017 Aug 24;170(5):956-972.e23 Screen identifies bromodomain protein ZMYND8 in chromatin recognition...

...constitutively active CMV promoter. Constructs were then verified by both diagnostic restriction digest and colony...generated mAb and the R-mAb, with the R-mAb being verified when the labeling matched that which was observed...

...optimized genetic screening technique, researchers identified a synthetic lethal interaction between MARCH5... available separately. The proteins, which are purified via histidine tags, are optimized for SDS-PAGE...

... For an estimate on availability date or to be notified when a particular catalog item is available for... the vector name and serotype you’d like to be notified about. Chemogenetics Plasmid Serotype Name ...

...molecular biology teamwork. The guide RNA (gRNA) specifies the editing target site on the DNA, the Cas domain...enzyme. After testing numerous TadA variants, they identified TadA-8e, which edits 590-fold faster than TadA...