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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 566 results for: FIE

Showing: 221 - 240 of 566 results

  3. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...and PfAgo, another thermophilic Argonaute. They identified NgAgo and showed that it can cleave plasmid and...removing 1-20 nucleotides from the cleavage site specified by the gDNA. Depending on your application, this...

  4. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...of the primer that binds to the sequence to be amplified (usually 18-21bp) When selecting restriction... primer that hybridizes to the sequence to be amplified (the ORF in this case), not the Tm of the entire...

  5. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ... as a seizure disorder.” In 2012, researchers identified the gene responsible for the disease as ATP1A3...specifically, they’ve proposed to use plasmids with modified nickase Cas9 protein in order to target the specific...

  6. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...et al. made the surprising discovery that both purified CPP-tagged protein and an untagged control could...subsequently enter the nucleus and cleave the specified target. Figure adapted from D’Astolfo et al. ...

  7. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...Identifying a host institute or lab So, you’ve identified a gap or need in your research and decided you...of these situations apply to you? Once you’ve identified one or more labs that you would like to visit...

  9. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...inhibitors cycloheximide and puromycin, Halstead et al. verified that translation is necessary for removal of GFP...the mCherry labeled mRNA and keeps it in a single field of view for constant tracking. You can then directly...

  10. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ... biological processes is highly useful in other fields, including synthetic biology. Addgene depositors...in optimizing TCSs. Over 75,000 TCSs have been identified in bacteria, and with similar optimization methods...

  11. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...today, where he helps researchers in a variety of fields develop RNAi screens to answer their own research...In the case of CRISPR, the hype was completely justified… it has been just an incredibly useful technology...

  12. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...student or postdoc. Although the chosen research field is important, the end goal during this time is to...like a scientist. Of course, the specific research field should be interesting; however, this should (in ...

  13. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ... end_module_attribute %}{% module_attribute "field_types" is_json="true" %}{% raw %}{"button_text":"text...end_module_attribute %}{% module_attribute "wrap_field_tag" is_json="true" %}{% raw %}"div"{% endraw %...

  14. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...SLIC cloning process, a gene of interest is PCR amplified to add 5′ and 3′ homology regions from the target...assembly with SLIC. Multiple fragments are PCR amplified to add 5′ and 3′ homology regions to the adjacent...

  15. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...hamsters, although no cancer-connection has been identified in humans to date. Over fifty serotypes of human-infecting...human-infecting adenovirus have been identified so far! Adenovirus generally has high immunogenicity, ...

  16. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...understand brain function So far scientists have identified 180 brain regions. Both this geography and the...all by this serotype. Nichole Beebe in Brett Schofield’s lab demonstrated that pathways projecting to ...

  17. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...constitutively active CMV promoter. Constructs were then verified by both diagnostic restriction digest and colony...generated mAb and the R-mAb, with the R-mAb being verified when the labeling matched that which was observed...

  18. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...optimized genetic screening technique, researchers identified a synthetic lethal interaction between MARCH5... available separately. The proteins, which are purified via histidine tags, are optimized for SDS-PAGE...

  19. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...college. Prior to March 2020, internships were a fiercely competitive endeavor. Due to the COVID-19 pandemic...better, and their leadership status in their current field of expertise augments their authoritative voice....

  20. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ... For an estimate on availability date or to be notified when a particular catalog item is available for... the vector name and serotype you’d like to be notified about. Chemogenetics Plasmid Serotype Name ...
Showing: 221 - 240 of 566 results