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Showing: 221 - 240 of 553 results

  1. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...et al. made the surprising discovery that both purified CPP-tagged protein and an untagged control could...subsequently enter the nucleus and cleave the specified target. Figure adapted from D’Astolfo et al. ...

  2. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...Identifying a host institute or lab So, you’ve identified a gap or need in your research and decided you...of these situations apply to you? Once you’ve identified one or more labs that you would like to visit...

  3. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...inhibitors cycloheximide and puromycin, Halstead et al. verified that translation is necessary for removal of GFP...the mCherry labeled mRNA and keeps it in a single field of view for constant tracking. You can then directly...

  5. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ... biological processes is highly useful in other fields, including synthetic biology. Addgene depositors...in optimizing TCSs. Over 75,000 TCSs have been identified in bacteria, and with similar optimization methods...

  6. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...today, where he helps researchers in a variety of fields develop RNAi screens to answer their own research...In the case of CRISPR, the hype was completely justified… it has been just an incredibly useful technology...

  7. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...student or postdoc. Although the chosen research field is important, the end goal during this time is to...like a scientist. Of course, the specific research field should be interesting; however, this should (in ...

  8. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...SLIC cloning process, a gene of interest is PCR amplified to add 5′ and 3′ homology regions from the target...assembly with SLIC. Multiple fragments are PCR amplified to add 5′ and 3′ homology regions to the adjacent...

  9. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...hamsters, although no cancer-connection has been identified in humans to date. Over fifty serotypes of human-infecting...human-infecting adenovirus have been identified so far! Adenovirus generally has high immunogenicity, ...

  10. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...understand brain function So far scientists have identified 180 brain regions. Both this geography and the...all by this serotype. Nichole Beebe in Brett Schofield’s lab demonstrated that pathways projecting to ...

  11. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ... aren’t the only investigators in the chromatin field who have deposited with Addgene, but you’ll find...DW Cell. 2017 Aug 24;170(5):956-972.e23 Screen identifies bromodomain protein ZMYND8 in chromatin recognition...

  12. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...college. Prior to March 2020, internships were a fiercely competitive endeavor. Due to the COVID-19 pandemic...better, and their leadership status in their current field of expertise augments their authoritative voice....

  13. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...genome editing.  For a few reasons, Cpf1 is a simplified system for editing multiple targets compared ...s ability to process its own pre-crRNA arrays simplifies the crRNA cloning process. For cloning, Zetsche...

  14. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ... For an estimate on availability date or to be notified when a particular catalog item is available for... the vector name and serotype you’d like to be notified about. Chemogenetics Plasmid Serotype Name ...

  18. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...constitutively active CMV promoter. Constructs were then verified by both diagnostic restriction digest and colony...generated mAb and the R-mAb, with the R-mAb being verified when the labeling matched that which was observed...

  19. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...optimized genetic screening technique, researchers identified a synthetic lethal interaction between MARCH5... available separately. The proteins, which are purified via histidine tags, are optimized for SDS-PAGE...

  20. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...high number of background colonies carrying the unmodified plasmid. For insertions, it’s possible to use...No No Yes Yes DpnI Digestion Yes Yes No No Purified Enzymes Required No No Yes Yes Lab Made Competent...
Showing: 221 - 240 of 553 results