We narrowed to 354 results for: HRE

1. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

   Type

   Blog Post

   Published

   March 19, 2019, 1:31 p.m.

   ...voltage indicator Archon Article contributed by Shreya Vedantam  Listen to the voltage indicator podcast...

2. Reproducibility for Everyone: Lessons from an Open Science Collaboration

   Type

   Blog Post

   Published

   Feb. 28, 2019, 1:34 p.m.

   ... co-instructed the ASPB workshop and authored a three-part blog post on the materials that were covered...

3. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

   Type

   Blog Post

   Published

   Jan. 11, 2017, 3:30 p.m.

   ...Labor Standards Act (FLSA). This was due to the threshold at which salaried workers receive overtime payment...

4. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

   Type

   Blog Post

   Published

   Sept. 4, 2018, 12:53 p.m.

   ..., the synthesis of deoxychromoviridans requires three genes whose proteins are needed sequentially. When...

5. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

   Type

   Blog Post

   Published

   Oct. 29, 2019, 2:52 p.m.

   ...using genome engineering. Planarian worms cut into three segments “remember” which side should grow a head...

6. CRISPR 101: Cas9 vs. The Other Cas(s)

   Type

   Blog Post

   Published

   Dec. 8, 2022, 2:15 p.m.

   ... for: a wide range of DNA edits.   Cas3 – The shredder What sets Cas3 apart from Cas9 and almost all ...

7. Antibodies 101: Secondary Antibodies

   Type

   Blog Post

   Published

   Dec. 21, 2021, 2:15 p.m.

   ...requirements of the specific assay. For the latter three, secondary antibodies are indicated when the protein...

8. Transferable Skills Guide: Problem Solving

   Type

   Blog Post

   Published

   Nov. 30, 2021, 2:15 p.m.

   ...potential strategies to resolve the problem.  Step Three: Decide on a strategy and implement it.  Step Four...

9. What's New in CRISPR - November 2021

   Type

   Blog Post

   Published

   Nov. 18, 2021, 2:15 p.m.

   ...activity in mammalian systems has been in question. Three different labs have recently published work that...

10. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...encoded voltage indicator Article contributed by Shreya Vedantam Genetically encoded voltage indicators...

11. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type

    Blog Post

    Published

    Nov. 24, 2015, 3:30 p.m.

    ...Once they’d found suitable pairs, they constructed three components: GBPa-VP16 (activation domain); GBPb-...

12. New Tool for Lineage Tracing: The ClonTracer Library

    Type

    Blog Post

    Published

    Sept. 22, 2015, 2:30 p.m.

    ...for her post-doc in January 2012. She worked for three years as a post-doc in the Target ID and Validation...

13. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type

    Blog Post

    Published

    April 22, 2015, 2:06 p.m.

    ...function is in the shape of an S; there is a narrow threshold like a switch that turns on or off. CRISPR shows...

14. Career Coaching for Scientists: Why and Where Do I Find One?

    Type

    Blog Post

    Published

    Jan. 29, 2015, 1:25 p.m.

    ...has experience as a scientific recruiter. Chris Humphrey from Jobs on Toast is based in the United Kingdom...

15. Plasmids 101: Cre-lox

    Type

    Blog Post

    Published

    Jan. 13, 2015, 3:47 p.m.

    ...reciprocal crossover event. This event can have three general outcomes based on the location and orientation...

16. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    Published

    March 7, 2024, 2:39 p.m.

    ...location or efficiency. If you are just trying to shred a long non-coding RNA, cutting most anywhere within...

17. Hot Plasmids February 2024

    Type

    Blog Post

    Published

    Feb. 20, 2024, 2:45 p.m.

    ... al., 2023); then, when AAV-PHP.eB was injected three weeks later, that LY6A transgene rescued the enhanced...

18. CRISPR in the Clinic

    Type

    Blog Post

    Published

    Nov. 2, 2023, 1:15 p.m.

    ...currently in clinical trials includes Cas3 (the shredder member of the Cas family) as part of a cocktail...

19. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    Published

    Aug. 10, 2023, 1 p.m.

    ...reporters with different kinetic parameters. There are three GCaMP types based on their kinetics: GCaMPf (fast...

20. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    Published

    June 22, 2023, 1:15 p.m.

    ...production then proceeds by transfection of all three plasmids into a producer cell line, such as HEK-...