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TypeBlog PostPublishedApril 7, 2015, 4:21 p.m....upon the type of transcriptional regulator or the number of designed scaffold binding sites. With hundreds...
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Advice for Moving Into Sales After Your Science Postdoc
TypeBlog PostPublishedAug. 21, 2014, 3:15 p.m....what options lie ahead of me? Like a considerable number of researchers, I had high hopes of securing tenure... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog PostPublishedJan. 21, 2014, 3:08 p.m....Leadership Communication Networking As the number of qualified scientists entering the workforce ... -
Quantifying DNA? Here are Five DNA Quantification Methods to Consider
TypeBlog PostPublishedJuly 21, 2020, 1:15 p.m....used as an indicator of DNA purity. Ideally, this number should be between 1.8 and 2.0. The A260/A230 ratio... -
FPbase: A new community-editable fluorescent protein database
TypeBlog PostPublishedMay 16, 2018, 1 p.m....fpvis (complete spectral information, accession numbers, sequences and lineages, FRET efficiencies, additional... -
The Importance of High Titer for AAV Transductions
TypeBlog PostPublishedMarch 1, 2018, 2:11 p.m....to be released into suspension and lowering the number of viral particles that can be harvested from the... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog PostPublishedJune 23, 2022, 1:15 p.m....through a phase of crisis where they 'collect' a number of mutations.” Cystic fibrosis may not be the most... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog PostPublishedMarch 30, 2023, 1:15 p.m...., TFs into their respective families. Given the number of poorly studied TFs, this functional association... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog PostPublishedApril 9, 2020, 1:15 p.m....email Addgene at [email protected] with your order number and so it can be prioritized. ... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog PostPublishedMarch 14, 2019, 1:54 p.m....a higher transduction efficiency, i.e. the same number of AAV particles is able to infect more cells (... -
The MTA at Addgene
TypeBlog PostPublishedJuly 23, 2024, 2:17 p.m.... process. This is important to keep up with the number of requests and deposits in our repository. From... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog PostPublishedSept. 3, 2019, 1:15 p.m....the transcriptional regulation of many genes. A number of groups have designed means of studying the effects... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog PostPublishedApril 18, 2019, 12:53 p.m....visualized and results were consistent with copy number variation (CNV) analysis of the U2OS cells. ... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m....Applications of the Gal4/UAS system There are a number of fancy ways to use the Gal4/UAS system, and you... -
Science Career Options
TypeBlog PostPublishedFeb. 23, 2017, 1:30 p.m....currently have too many applicants compared to the number of available roles). Make your resume stand out... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog PostPublishedMarch 24, 2016, 2:30 p.m....of approximately 1%. This is by no means a large number, but it was attained without any of the error-prone... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog PostPublishedOct. 9, 2014, 3 p.m....extremely bright. In S. cerevisiae, we've tested a number of green and red fluorescent proteins with this... -
Plasmids 101: Screening Strategies Used in Plasmid Cloning
TypeBlog PostPublishedSept. 15, 2020, 1:15 p.m....biology, cloning a gene into your plasmid is often a numbers game. By using a variety of screening and selection... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m....transcription, or DNA repair, or replication, or any number of CRISPR applications and thought “OK, but how... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog PostPublishedDec. 15, 2017, 2:07 p.m....neuroscience and, like many biological fields the number potential of experiments enabled by physical, software...