We narrowed to 290 results for: Pol

1. Troubleshooting Your Plasmid Cloning Experiment

   Type

   Blog Post

   Published

   Sept. 24, 2019, 12:55 p.m.

   ...companies selling PCR reagents. Nowadays commercial polymerases are very efficient, but if you are having difficulties...

2. CRISPR 101: Cas9 vs. The Other Cas(s)

   Type

   Blog Post

   Published

   Dec. 8, 2022, 2:15 p.m.

   ...to detect variants such as single nucleotide polymorphisms. Fast facts – PAM requirement: none. Best ...

3. Summer SciComm: Preprints

   Type

   Blog Post

   Published

   Aug. 16, 2022, 1:15 p.m.

   ...comment online! Want the general public (say, policymakers or community organizations) to use your work...

4. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   Published

   April 22, 2015, 2:06 p.m.

   ...used to create any program you can imagine. The Apollo 11 mission circuitry that took a spaceship to the...

5. Career Coaching for Scientists: Why and Where Do I Find One?

   Type

   Blog Post

   Published

   Jan. 29, 2015, 1:25 p.m.

   ...PhDs and postdocs posted by Toronto-based Jennifer Polk of From PhD to Life. She runs great tweet chats,...

6. Educational Planarian Tools for Teaching Developmental Biology Techniques

   Type

   Blog Post

   Published

   May 24, 2018, 1:20 p.m.

   ...expression is activated through the  induction of T7 polymerase encoded in the E. coli genome (usually controlled...

7. Design Tips for Prime Editing

   Type

   Blog Post

   Published

   Jan. 23, 2025, 2:15 p.m.

   ... La to protect the end of the pegRNA. Adding 3′ polyU tracts to the end of pegRNAs (but not epegRNAs) ...

8. Antibodies 101: Stripping and Reprobing Western Blots

   Type

   Blog Post

   Published

   Aug. 1, 2024, 1:15 p.m.

   ...antibodies. You’ll want to set up your blot with a polyvinylidene difluoride (PVDF) membrane, which is sturdier...

9. Hot Plasmids and Viral Preps - January 2021

   Type

   Blog Post

   Published

   Jan. 5, 2021, 2:15 p.m.

   ... CRISPR human genome-wide library deposited by Leopold Parts. This library delivers 2 randomly paired ...

10. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    Published

    Oct. 6, 2020, 1:15 p.m.

    ... P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong ...

11. Open Resources and Plasmid Tools For Studying C. elegans

    Type

    Blog Post

    Published

    July 18, 2019, 12:55 p.m.

    ... C. elegans 3’UTRs structures, alternative polyadenylation for all protein coding mRNAs, and annotations...

12. A Look at Addgene's QC Process

    Type

    Blog Post

    Published

    May 6, 2025, 1:15 p.m.

    ... and biological. Even the most high-fidelity polymerases can make mistakes and lead to the accumulation...

13. CRISPR in the Clinic

    Type

    Blog Post

    Published

    Nov. 2, 2023, 1:15 p.m.

    ...is caused by a variety of single nucleotide polymorphisms (SNPs) in the TTR gene and is molecularly characterized...

14. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    Published

    Dec. 1, 2015, 3:30 p.m.

    ...technology park, it enjoys preferential governmental policies for a high-tech development zone so many science...

15. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type

    Blog Post

    Published

    Jan. 21, 2015, 1:30 p.m.

    ...-aggressives, who are usually antagonistic and impolite, Complainers, who spend most of their time moaning...

16. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    Published

    Jan. 10, 2017, 3:37 p.m.

    ..., Cas9 forms a steric block that prevents RNA polymerase from efficiently transcribing the gene. This ...

17. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    Published

    March 28, 2018, 6:52 p.m.

    ...catalytically dead SpCas9 (dCas9) fused to the bacterial polymerase subunit ω. This construct is designed to activate...

18. Magnetic Control of Proteins: More than a Dream

    Type

    Blog Post

    Published

    Sept. 24, 2024, 1:15 p.m.

    ...References and resources References Duret, G., Polali, S., Anderson, E. D., Bell, A. M., Tzouanas, C....

19. Viral Vectors 101: Producing Your rAAV

    Type

    Blog Post

    Published

    July 16, 2024, 1:15 p.m.

    ...rAAV from your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of 40% PEG solution...

20. Hot Plasmids and Viral Preps - May 2021

    Type

    Blog Post

    Published

    May 18, 2021, 1:15 p.m.

    ...transporting proteins in yeast by using murine polyomavirus virus-like particles (MPyV VLPs ). This artificial...