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We narrowed to 610 results for: REV

Showing: 221 - 240 of 610 results

  1. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...the limitations associated with SpCas9. Cas13a (previously referred to as C2c2), has several unique properties...adopts an enzymatically “active” state rather than reverting to an inactive state like Cas9 or Cpf1. As a result... Recombinase Polymerase Amplification (RPA) or Reverse-transcription RPA (RT-RPA), respectively. Amplified...

  2. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...terrible disease that is, unfortunately, completely preventable. Mainly caused by exposure to asbestos, mesothelioma...This drug (known as an angiogenesis inhibitor) prevents the growth of new blood vessels required to sustain...treatment get, there still remains the problem of prevention. Asbestos exists pretty much everywhere, still...

  3. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...Incorporating reverse transcriptase (RT) variants, PE6a and PE6b are 500–800 bp smaller than previous editors...reprogram delivery vehicles like lentiviral vectors, CreVLPs, and eVLPs for in vivo and in vitro applications...

  4. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ... in a separate backup liquid nitrogen store to prevent loss from equipment malfunctions. One Master vial...approach, the many mistakes of the past can be prevented and we can progress faster to desired clinical...years on: the good, the bad and the ugly."Nature Reviews Cancer 2.4 (2002): 315-319. PubMed PMID: 12001993...

  5. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...Site-Specific Integrase Gene Editing (PASSIGE), their previously-reported technique for precisely integrating ...P. Bentley The Weissman and Gilbert Labs have previously deposited their CRISPRoff system — a programmable...the cells you are studying. Antibodies can help reveal cellular identities by targeting marker proteins...

  6. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...efficiently by (1) improving editing accuracy by preventing re-editing and (2) optimizing “cut-to-mutation... was that these “blocking mutations” mutations prevent CRISPR from re-cutting the target sequence once... mutation incorporation probabilities our data revealed, and found that for generating a homozygous HDR...

  7. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ... details. You DO NOT need to present a detailed review of your field or the finer points of your techniques...avoid the temptation to provide too many details. Reverse engineer your talk. Identify the key points you...This means that once you get an interview, your previous success will not necessarily translate into getting...

  8. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...(Pfeiffer et al., 2010). Yeast (Saccharomyces cerevisiae) Yeast, one of the simplest eukaryotic organism...190-195. Liu, Wei, et al. "From Saccharomyces cerevisiae to human: The important gene co‑expression modules...in therapeutic drug discovery." Pharmacological reviews 63.2 (2011): 411-436. PubMed PMID: 21415126. PubMed...

  9. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...the problematically low fluorescent intensity of previous versions of Brainbow. Developed from Brainbow ...visual system, which has been difficult to study previously (Hadjieconomou et al., 2011). Cell tracing in...distances across multiple fields of view, whereas previous Brainbow technologies were useful for discriminating...

  10. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...cells compared to monomers, although this can be prevented via antibiotic selection (Crozat, 2014). How to... multimerization, and in some cases completely prevent the formation of plasmid multimers (Bedbrook & ...overgrowing the bacterial culture Another way to prevent plasmid multimerization is to avoid growing the...

  11. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...gathering data. Former lab members can give open, revealing information about the culture and lab head. While...intimidation, sexual harassment Isolation – including preventing access to opportunities, physical or social isolation...record notes and observations so you have data to review when making a decision. If you don’t see a culture...

  12. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...biased and unbiased approaches. As suggested in a review by Tycko et al., 2016, unbiased sequencing and ...High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity." Nature...specificities of CRISPR-Cas9 nucleases." Nature Reviews Genetics17.5 (2016): 300-312. PubMed PMID: 27087594...

  13. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...inhibitor of uracil DNA glycosylase (UGI) to dCas9 prevents base excision repair, which would change the U...of inosine from DNA is likely infrequent, thus preventing the induction of base excision repair. In terms...improved base editing capacity, even at sites previously difficult to target. ABE8s can achieve 98-99%...

  14. Enabling high school research at the Journal of Emerging Investigators

    Type
    Blog Post
    Published
    Sept. 5, 2018, 12:01 p.m.
    ...that enables high school students to publish peer-reviewed scientific research. You’ll meet some of the folks...mouth and personal networking!) and the manuscript review/submission process at JEI. Chapter 3 - JEI target...

  15. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...DeckTheLab and tagging us, @addgene to be judged! In previous years, scientists have decorated their labs with...on the entries, select up to three winners, and reveal the winning entries on Twitter and Instagram! Happy...

  16. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    Published
    March 2, 2021, 2:15 p.m.
    ...microbiology lab every year for over 20 years. Previously, the lab used three different species for the...continues until all quadrants are used, hopefully revealing single, isolated colonies in the last quadrant...

  17. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ... expression of specific genes in the brain in reversible fashion. And that’s exactly what the LITEs now...response can be graded with light dose and is fully reversible after light retraction. Finally, because light...

  20. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ... stage is often much easier said than done. As reviewed in our Plasmids 101 series, proteins are overexpressed...protein expression and purification: a comprehensive review of affinity tags and microbial applications." Biotechnology...
Showing: 221 - 240 of 610 results