We narrowed to 478 results for: SEC

...recombineering experiment (Figure 2). In the following sections, key steps that differ from traditional restriction...removed from the chromosome of the host E. coli by a second lambda red recombination event. Alternatively, ...

... you may not find in the materials and methods sections of optogenetics papers. Optogenetics step one...channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function. Nature methods....

...and corresponding Multi-rAb CoraLite® Plus 647 secondary antibodies. Antibody staining was quantified using...histograms show cells that have only been stained with secondary antibodies, showing the level of background staining...

...design resources in the References and Resource section. Validation Once designed, your primer and probe...amplicons; if your amplicon is AT-rich or has a secondary structure, you may see a double peak even with...

...N-glycosylase (UNG) during base excision repair. Adding a second copy of the UNG inhibitor, UGI, increases base ...BE4-Gam; AID, CDA1 and APOBEC3G BE3 variants   A second copy of UGI improves product purity. Gam decreases...

... by the Gradinaru lab. For simplicity, in each section we’ll list the Gradinaru capsids first, before ... levels to AAV9 (Stanton et al., 2023). PAL2, a second-generation PAL capsid, shows enhanced transduction...

...edition has been fully updated with twelve new sections, including our popular "Dimers and Multimers" ...

...to optimizing AAVs in your experiment.      Our second edition includes new and updated content in a clean...

...processing (Nextflow), cloud infrastructure, and secure collaboration.  In that post, we talked about Addgene...

...gave to me…  An Antibodies 101 eBook!  🎶On the second day of Antibody, Addgene gave to me…  A recombinant...

...In the second episode of our Hot Plasmids podcast series, you'll learn about new red fluorescent proteins...

...miniature CRISPR-Cas system for mammalian use. On the second day of CRISPR, Addgene gave to me: engineered pegRNAs...

... gave to me: a primer on prime editing.  On the second day of CRISPR, Addgene gave to me: SHERLOCK and...

...winners of the 2019 contest. Agar art For the second year in a row, agar art is one of the winning decorations...

...exceptions were made by the US Bureau of Industry and Security, pursuant to the international controls prescribed...

...us at Addgene's booth, #3422, in the nonprofits section.  The Booth What will you find at Addgene's booth...

...Joachim Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre...

...available through the US Bureau of Industry and Security. If you have any questions about this or other...

... This plasmid has been used in studies of the secretome, ubiquitin ligase, cellular senescence, autophagy...

...you think about the calculator in the comments section below. References 1. Choi, Vivian W., et al. "...