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We narrowed to 478 results for: SEC

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Showing: 221 - 240 of 478 results

  1. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ... but with random combinations of the different sections. Structure-guided directed evolution If you desire...antibodies that target families of cell surface and secreted proteins (glypicans, integrins, and more coming...

  2. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ... you may not find in the materials and methods sections of optogenetics papers. Optogenetics step one...channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function. Nature methods....

  3. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...and corresponding Multi-rAb CoraLite® Plus 647 secondary antibodies. Antibody staining was quantified using...histograms show cells that have only been stained with secondary antibodies, showing the level of background staining...

  4. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...design resources in the References and Resource section. Validation Once designed, your primer and probe...amplicons; if your amplicon is AT-rich or has a secondary structure, you may see a double peak even with...

  5. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...N-glycosylase (UNG) during base excision repair. Adding a second copy of the UNG inhibitor, UGI, increases base ...BE4-Gam; AID, CDA1 and APOBEC3G BE3 variants   A second copy of UGI improves product purity. Gam decreases...

  6. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ... by the Gradinaru lab. For simplicity, in each section we’ll list the Gradinaru capsids first, before ... levels to AAV9 (Stanton et al., 2023). PAL2, a second-generation PAL capsid, shows enhanced transduction...

  7. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    Published
    Sept. 5, 2023, 1:15 p.m.
    ...edition has been fully updated with twelve new sections, including our popular "Dimers and Multimers" ...

  8. Viral Vectors 101 eBook 2nd Edition

    Type
    Blog Post
    Published
    July 11, 2024, 1:15 p.m.
    ...to optimizing AAVs in your experiment.      Our second edition includes new and updated content in a clean...

  9. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...processing (Nextflow), cloud infrastructure, and secure collaboration.  In that post, we talked about Addgene...

  10. The Twelve Days of Antibodies

    Type
    Blog Post
    Published
    Jan. 6, 2025, 5:17 p.m.
    ...gave to me…  An Antibodies 101 eBook!  🎶On the second day of Antibody, Addgene gave to me…  A recombinant...

  12. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...miniature CRISPR-Cas system for mammalian use. On the second day of CRISPR, Addgene gave to me: engineered pegRNAs...

  13. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    Published
    Jan. 17, 2020, 3 p.m.
    ...winners of the 2019 contest. Agar art For the second year in a row, agar art is one of the winning decorations...

  14. The Twelve Days of CRISPR

    Type
    Blog Post
    Published
    Dec. 22, 2020, 2:15 p.m.
    ... gave to me: a primer on prime editing.  On the second day of CRISPR, Addgene gave to me: SHERLOCK and...

  15. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ...us at Addgene's booth, #3422, in the nonprofits section.  The Booth What will you find at Addgene's booth...

  17. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...available through the US Bureau of Industry and Security. If you have any questions about this or other...

  19. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ... This plasmid has been used in studies of the secretome, ubiquitin ligase, cellular senescence, autophagy...

  20. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ...Hot Plasmids topics, let us know in the comments section below. Hot plasmids covered in this segment Hot...
Showing: 221 - 240 of 478 results