...Tropism and Axonal Transport of AAV Serotypes 1, 2, 5, 6, 8 and 9 in the Mouse Brain. PLoS ONE 8:e76310....Pseudotyped with Viral Capsids from Serotypes 1, 2, and 5 Display Differential Efficiency and Cell Tropism after...the most common concerns we get here at Addgene about this system is off-target or spurious expression...cells that don’t express Cre or Flp, what can we do about it?  Test the serotype and promoter The first step...

...Learn How You Can Use Video to Share Your Research 5 Reasons to Use Reddit for Science Communication 10...finally decide the LEGO laboratory was good enough. There are no pre-made LEGO laboratory sets so we had to ...everything now is about the plot. This part is easy, after all, who working in a laboratory has never faced...dysregulated water bath boiling your experiments, just about anything can disrupt your bench work and sometimes...to show people the very human struggles in the laboratory routine. We hope that sharing and laughing at...can learn with each other through globalized, collaborative thinking. LEGO and science: The potential is...while opening a LEGO set with my husband, I thought about how the limitless design possibilities of the LEGOs...

...blogosphere is to read and comment on their blogs. 5)   Local science museums are a great place to find...take on collaborative roles in academia, and that means it’s important to know how to talk about science...talking about and sharing some science on the one you already have. Read my earlier post about Scientists...important for scientists to get out there and talk about their work. To tell others why that work is cool... than ever before for scientists to communicate about their work and their interest in science more generally...already make it a habit to talk – really talk – about the research you are doing in the lab with your ... the details of your science but also something about how science really works. 2)   Read science writing...

...transgene. On a plasmid map, promoters are upstream or 5’ of the gene they control. Promoters can be organism...the Addgene blog Learn more about basic viral vector elements Read about four considerations for the ...Key elements of an AAV plasmid First let’s talk about the key parts of an AAV plasmid. Inverted terminal...routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ITR loss, it’s best... Other cis-regulatory elements Next let’s talk about some of the elements that aren’t required parts ...the perfect AAV tool Learn about synthetic promoter AAVs for cell-type expression in retinal cells Resources...

...bioengineering 37.10 (1991): 989-993. PubMed PMID: 18597326. 5. Shen, Chun Fang, and Amine Kamen. "Hyperosmotic pressure...resources on Addgene.org Learn about Addgene's viral service Learn about viral production Find viral protocols... By now you have probably heard about Addgene’s viral service but did you know that Addgene also performs... approach can be adapted by vector cores and laboratories alike as an easy and inexpensive method to improve...Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production." Biotechnology and ...

...Biorender workspace. Free Biorender accounts can store 5 workspaces at a time and workspaces are only downloadable... for the Addgene blog, I was focused on writing about new scientific techniques and cool plasmids. Creating... graphics were usually the last thing I thought about when writing posts. Since then I’ve realized my ...know of a tool or resource not mentioned? Tell us about it in the comments section!  Additional resources...scientists Find science career blog posts Learn about other lab software ...

... The sample was separated into smaller samples of 5-10 cells each for sequencing, allowing for lower complexity...Addgene blog Learn about other Cas proteins Read all of our CRISPR blog posts Read about GeoCas9, another... able to eliminate the need for axenic (pure) laboratory cultures to study these microbes. From 16S rRNA...by flexibly deploying IgnaviCas9 without concern about its stability under harsh conditions. One example...

...Blue-White screening could be a potential solution. 5. Cpf1: A New Tool for CRISPR Genome Engineering While...eyes open for any new technologies that will bring about the next revolution in biological research. We’re...Plasmids 101: Control Plasmids Need help thinking about the types of controls you’ll need for your next ...Addgene Browse Our CRISPR Resources Learn More about Viral Vectors Browse the Repository  ...

...you choose the best CRISPR software for your needs 5 great apps for lab life  Sharing your lab protocols...particular tasks, but they tend to be scattered about the internet. To help you keep your virtual molecular...other applications Primer design tools Many of the above-listed plasmid mapping tools have built-in primer...

... patterns generated by the nickases; D10A creates 5’ overhangs and H840A creates 3’ overhangs in a PAM-out...Additional Resources on the Addgene Blog Learn about Homology Directed Repair 3 Tips to Improve HDR Efficiency...

...increased my autonomy and satisfaction with my work.  5. Do you enjoy bench work? It’s easier to stay at the... Resources Resources on the Addgene blog Learn about career planning resources What Do I Do Now? Academic...

... c-myc proto-oncogene product. Mol Cell Biol. Dec;5(12):3610-6.(1985). doi: 10.1128/mcb.5.12.3610-3616...Brian O'Neill  The François St-Pierre lab and collaborators have successfully developed an improved voltage...

...overhangs depending upon whether they are on the 3’ or 5’ end of the gene of interest allowing for insertion...of DNA that can move around in a genome. In a laboratory setting, transposons can be used to both introduce...

...single fluorescent biosensor." Scientific reports 5 (2015): 12397. PubMed PMID: 26215030. PubMed Central...measurement and it provides limited information about what materials or organelles are being targeted ...

...Netherlands. Transformation of HEK cells with adenovirus 5 DNA was postdoc Frank Graham’s 293rd experiment, hence... currently assumed that the cells came from an aborted fetus. HEKs have an unusual ploidy and are considered...

... raising salaries as their website implies. These 5 organizations, according to the NSF data, account ...the Addgene Blog How did this change in labor laws come about? In March 2014, the White House issued a...must be above this level, and certain job duty tests must be met. In the Department of Labor’s general... to $47,476 per year, under updates to the Fair Labor Standards Act (FLSA). This is having a major effect...ensuring that either all affected scientists are paid above this threshold or for tracking the hours that these... a memorandum to the Secretary of Labor to update the FLSA, which had not seen revisions since 2004. In... In July 2015, the Department of Labor issued a Notice of Proposed Rulemaking, soliciting feedback by ...

...more important to start early. Think about your plan for the next 5 years. Does an academic career work...patent application processes? How about facilitating a collaboration between research groups? Perhaps ... a good job summarizing what a resume should be about. With a CV, information is usually presented chronologically...developed through your research? Don't simply write about the research you did. Emphasize the skills. If you... structures, then you might know a thing or two about computer modeling. Make sure to focus on these skills...the job you are applying for, in which case talk about your research, as an understanding of the topic ...you some advantage over other candidates. Write about your transferable skills Some of the skills you ...

... entering the specifics of your plasmids. Figure 5: Click the "Enter Data" button to add your plasmid...Enter the growth and distribution information. Step 5: Verification On this page you will see a preview ...annotate on this page. Figure 7: Enter information about the gene/insert(s). Step 3: Cloning Information ...

...focusing on a task, like writing, and then take a 5-minute break. Each 30-minute interval is called a ...frequency of use in black. This paper talks more about the development of the De-jargonizer.   Academic...with others which makes it easier to work with collaborators. Zotero is a desktop based program that’s compatible...episode 2 "Organizing Literature" : I found out about Zotero here Dear Grad Student podcast episode 2 ...Programming, & Productivity": This podcast talks about Mendeley, Zotero, and other citation managers and...the pomodoro technique": This podcast talks more about using the Pomodoro method in grad school Additional...

...Elsevier BV. https://doi.org/10.1016/s0021-9258(18)99982-5 More resources on the Addgene blog Antibodies 101:...flexibility needed for multiplexing. In the case above, you can’t costain with your desired antibodies ...multiplexing with chimeric antibodies. In the case above you should use an anti-rat IgG1 secondary antibody...this blog post has answered all of your questions about the utility of isotype conversions and chimeric ..., Bright, S. M., Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R., King, D. J., Norris, S. H., &...