...significant hit across the E. coli genome as a negative control. Read the paper in Nature Communications Find...

...has the advantage that CRISPR efficiency can be controlled by manipulating complementarity between the gRNA...

...noises and flashing lights - that sent average, control mice into depression. In case you were wondering...

...circuits, allowing for tighter spatio-temporal control of gene expression within a cell. Thanks to the...

...with the lentivirus HIV-1. Today, HIV-1 can be controlled with antiviral therapies such that the virus ...infection period rather than the ART-induced viral control found in most HIV-1 patients. Obstacles for CRISPR...

... they do so by activating signaling pathways controlling properties like growth, survival, and differentiation...

...TOPFlash, scramble shRNA for use as a negative control, and empty backbones such as pBABE-puro for retroviral...

...effect on cell viability compared to a wild-type control. Approximately ~22% of the mutations, however, ...

...technique from David Liu’s lab that gives more control and flexibility to CRISPR editing. Using a fusion...

...documents from anywhere, but also allow you to control who can edit the documents and track the revision...

...protein and a tag, such as an NLS or NES, enables control over localization. Inserting PhoCl between an enzyme...enzyme and an inhibitor domain similarly allows for control of enzymatic activity. In addition, proteins can...expression. Expression of toolkit components can be controlled by a variety of promoters for versatile and robust...

...Epigenetic modifications are an additional layer of control over gene expression that go beyond genomic sequence...catalytically dead modifiers are also available as controls. For an up-to-date list of CRISPR epigenetic tools...

...the number of integration copies can be tightly controlled by titrating the transposase to transposon ratios... levels of your gene of interest, as needed. Controlling the expression levels of your gene may be particularly...

...FRT, with AAV vectors are common approaches to control transgene expression by restricting expression ...

...Using CRISPR/Cas9 and Plasmids 101: NGS Quality Control for Pooled Libraries Day 12: New website resources...

...synthesize new plasmids quickly and Addgene’s quality control and archiving system allowed us to handle and distribute...

...Concept These systems were benchmarked on positive control genes in CD4+ and CD8+ T cells. To demonstrate ...

...sequencing, setting new standards for plasmid quality control. Through our partnership with the Institute for...

...Packaged on Request prep and run the same quality control assays we run with all of our in-stock viral preps...

...through any search, ordering, technical, or quality control issues. Improving customer support with self service...