We narrowed to 389 results for: control
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TypeBlog PostPublishedMarch 26, 2015, 3:24 p.m....your plasmids, storing them, providing quality control and customer support, making plasmids easy for ...
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CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog PostPublishedSept. 28, 2020, noon... of knockdown and developed new, more tightly controlled, Tet responsive promoters to avoid leaky activation...This approach provides a more granular level of control where individual bases can be modified. Cytidine...as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell 152:1173–1183 . https:... -
What's New in CRISPR - May 2019
TypeBlog PostPublishedMay 28, 2019, 12:49 p.m....significant hit across the E. coli genome as a negative control. Read the paper in Nature Communications Find... -
What's New in CRISPR - Spring 2019
TypeBlog PostPublishedMarch 21, 2019, 12:47 p.m....has the advantage that CRISPR efficiency can be controlled by manipulating complementarity between the gRNA... -
How Exercise Purges Stress and Keeps Depression at Bay
TypeBlog PostPublishedNov. 25, 2014, 4:02 p.m....noises and flashing lights - that sent average, control mice into depression. In case you were wondering... -
Designer PUF Proteins for Any RNA Target
TypeBlog PostPublishedJune 17, 2014, 7:57 p.m....circuits, allowing for tighter spatio-temporal control of gene expression within a cell. Thanks to the... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog PostPublishedMay 10, 2016, 2:30 p.m....with the lentivirus HIV-1. Today, HIV-1 can be controlled with antiviral therapies such that the virus ...infection period rather than the ART-induced viral control found in most HIV-1 patients. Obstacles for CRISPR... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog PostPublishedMarch 22, 2016, 2:30 p.m.... they do so by activating signaling pathways controlling properties like growth, survival, and differentiation... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog PostPublishedJan. 8, 2014, 3:10 p.m....TOPFlash, scramble shRNA for use as a negative control, and empty backbones such as pBABE-puro for retroviral... -
A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog PostPublishedMay 7, 2019, 12:39 p.m....effect on cell viability compared to a wild-type control. Approximately ~22% of the mutations, however, ... -
Our Most Popular Blog Posts from 2019
TypeBlog PostPublishedDec. 31, 2019, 2:26 p.m....technique from David Liu’s lab that gives more control and flexibility to CRISPR editing. Using a fusion... -
Three Tips to Organize Your Lab Notebooks in the New Year
TypeBlog PostPublishedJan. 2, 2014, 3:44 p.m....documents from anywhere, but also allow you to control who can edit the documents and track the revision... -
15 Hot Plasmids from 2017
TypeBlog PostPublishedJan. 9, 2018, 3:02 p.m....protein and a tag, such as an NLS or NES, enables control over localization. Inserting PhoCl between an enzyme...enzyme and an inhibitor domain similarly allows for control of enzymatic activity. In addition, proteins can...expression. Expression of toolkit components can be controlled by a variety of promoters for versatile and robust... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog PostPublishedJune 24, 2020, 5:45 p.m....Epigenetic modifications are an additional layer of control over gene expression that go beyond genomic sequence...catalytically dead modifiers are also available as controls. For an up-to-date list of CRISPR epigenetic tools... -
Hot Plasmids: Spring 2025
TypeBlog PostPublishedJune 3, 2025, 1:15 p.m....channelrhodopsin-2 or fluorescent reporter dTomato under the control of one of seven enhancers specific for different...expression of dTomato reporter (magenta) under the control of the BiPVe3 enhancer and parvalbumin (PV) immunostaining... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog PostPublishedMay 31, 2016, 3:30 p.m....the number of integration copies can be tightly controlled by titrating the transposase to transposon ratios... levels of your gene of interest, as needed. Controlling the expression levels of your gene may be particularly... -
Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies
TypeBlog PostPublishedNov. 27, 2019, 2:03 p.m....FRT, with AAV vectors are common approaches to control transgene expression by restricting expression ... -
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog PostPublishedJune 23, 2020, 1:15 p.m....synthesize new plasmids quickly and Addgene’s quality control and archiving system allowed us to handle and distribute... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog PostPublishedDec. 12, 2017, 3:13 p.m....Using CRISPR/Cas9 and Plasmids 101: NGS Quality Control for Pooled Libraries Day 12: New website resources... -
Reflections and Looking Ahead
TypeBlog PostPublishedFeb. 25, 2025, 2:15 p.m....sequencing, setting new standards for plasmid quality control. Through our partnership with the Institute for...