We narrowed to 516 results for: gan

... number of CRISPR plasmids for that particular organism available at Addgene. Percentages represent the...used to deliver CRISPR components to cells or organisms under study. In the past we’ve left the arduous...

...cleavage. Secondly, fluorescence imaging of living organisms can be challenging due to issues with tissue autofluorescence...dynamics of apoptosis both in vitro and in living organisms to better study and understand these diseases ...

...tools by increasing gRNA stability. First, they began with the Broccoli aptamer system to visualize the... will recruit the HaloTag with red fluorescent ligands to the loci of interest. Dual color detection In...

...extreme life on earth including extremophile microorganisms, biomarkers, and recently, cancer research ...Addgene has partnered with various community organizations and workforce projects to reach audiences beyond...

... are needed. All-Company Meetings: As your organization grows, so will the length of your group meetings... strategies and find out what works for your organization. Ask for feedback from your colleagues. When...

... cut the target. Cas9 homologs from different organisms or Cas9 mutants developed in a variety of labs...EQR variant 3' NGAG SpCas9 VQR variant  3' NGAN or NGNG  xCas9 3' NG, GAA, or GAT SpCas9-NG ...

...develop to reach their full potential in the organization. This will result in more engaged, successful...Series Learn How Volunteering at a Professional Organization Can Help You Develop Leadership Skils Set Concrete...

...mutagenizing a population (typically cells or small organisms) and then assaying for a functional or phenotypical... events. To put it another way, only cells or organisms with the desired phenotype will survive and be...

...systemically transduce and then retransduce a model organism such as mice, e.g. first at 2 months, then at ...field has settled on ‘best practices’ for model organisms.    Q11: How consistent is expression is when ...

... 2018 Chromosome Architecture and Chromosome Organization Keystone Symposium. As it happens, there are...Keystone Chromosome Architecture and Chromosome Organization. These aren’t the only investigators in the ...

...for easy genome engineering in species like C. elegans and Drosophila. Light inducible promoters Light...transcription machinery differs between cell types or organisms, promoters are similarly variable. Bacterial promoters...

...Neuroscience covers many subfields When I first began looking into the details of SfN17, I expected that...addition, there was an entire story-telling session organized by Story Collider and which featured a talk about...

... environment, all while reciting their favorite slogan, “look how cool the brain is!" Using retrograde...account since activation with chemogenetic specific ligands activates both neurons at the injection site and...

...inflammation for therapeutic benefit ”as stated by the organizers. My overall impression from this conference...dynamic microvillar search and stabilization during ligand detection by T cells." Science 356.6338 (2017):...

...different organisms and engineered Cas9 variants that were able to target different PAM sites (NGG, NGAN, NGNG...

...processing machinery present in almost every living organism. Precursor, or pre-tRNAs are cleaved by RNase ... illustrated the power of polycistronic gene organization, and its translation to CRISPR/Cas9 shows great...

...understanding of the physiology and pathology of the basal ganglia in movement disorders.   References and Resources...protein-coupled receptors potently activated by an inert ligand. Proceedings of the National Academy of Sciences...

...proteins are retained in the interphase and bottom, organic layer. The top aqueous layer is extracted and RNA...layer to avoid carryover of DNA from the bottom, organic layer. Another trick is to precipitate the RNA ...

...been used to construct plasmids for use in model organisms such as E. coli and yeast. Because this approach...2019. This novel tool is practical in cell and organ culture, as well as cell lineage studies. Eisenhoffer...

... binding domains fused to either the hER or hPR ligand binding domains and the MSN2 transactivation domain...Transcription factor activation via the appropriate ligand - estradiol for hER or progesterone for hPR - leads...