We narrowed to 515 results for: gan
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TypeBlog PostPublishedJan. 12, 2021, 2:15 p.m....If appropriate, invite them to speak for your organization, mentoring group, or department. Even if it ...gestures are memorable! Attend events for one organization or go to the same conferences/meetings over ...
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Science Career Options
TypeBlog PostPublishedFeb. 23, 2017, 1:30 p.m....that requires travel can show that you are self organised, reliable, and can work independently. Practical...involves traveling, showing that you are a highly organised and experienced traveler by citing specific examples... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog PostPublishedDec. 6, 2016, 3:30 p.m.... number of CRISPR plasmids for that particular organism available at Addgene. Percentages represent the...used to deliver CRISPR components to cells or organisms under study. In the past we’ve left the arduous... -
How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m.... are needed. All-Company Meetings: As your organization grows, so will the length of your group meetings... strategies and find out what works for your organization. Ask for feedback from your colleagues. When... -
Components of CRISPR/Cas9
TypeBlog PostPublishedFeb. 2, 2016, 5 p.m.... cut the target. Cas9 homologs from different organisms or Cas9 mutants developed in a variety of labs...EQR variant 3' NGAG SpCas9 VQR variant 3' NGAN or NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG ... -
Management for Scientists: Delegating is Key
TypeBlog PostPublishedFeb. 20, 2015, 8:30 p.m....develop to reach their full potential in the organization. This will result in more engaged, successful...Series Learn How Volunteering at a Professional Organization Can Help You Develop Leadership Skils Set Concrete... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m.... 2018 Chromosome Architecture and Chromosome Organization Keystone Symposium. As it happens, there are...Keystone Chromosome Architecture and Chromosome Organization. These aren’t the only investigators in the ... -
Plasmids 101: Inducible Promoters
TypeBlog PostPublishedJan. 18, 2018, 2:34 p.m....for easy genome engineering in species like C. elegans and Drosophila. Light inducible promoters Light...transcription machinery differs between cell types or organisms, promoters are similarly variable. Bacterial promoters... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog PostPublishedDec. 15, 2017, 2:07 p.m....Neuroscience covers many subfields When I first began looking into the details of SfN17, I expected that...addition, there was an entire story-telling session organized by Story Collider and which featured a talk about... -
AAV Q&A with Tim Miles
TypeBlog PostPublishedMay 17, 2022, 1:15 p.m....systemically transduce and then retransduce a model organism such as mice, e.g. first at 2 months, then at ...field has settled on ‘best practices’ for model organisms. Q11: How consistent is expression is when ... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog PostPublishedJuly 6, 2017, 1:59 p.m....inflammation for therapeutic benefit ”as stated by the organizers. My overall impression from this conference...dynamic microvillar search and stabilization during ligand detection by T cells." Science 356.6338 (2017):... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog PostPublishedApril 27, 2023, 1:15 p.m.... environment, all while reciting their favorite slogan, “look how cool the brain is!" Using retrograde...account since activation with chemogenetic specific ligands activates both neurons at the injection site and... -
Plasmids 101: Screens vs. Selections
TypeBlog PostPublishedNov. 10, 2022, 2:15 p.m....mutagenizing a population (typically cells or small organisms) and then assaying for a functional or phenotypical... events. To put it another way, only cells or organisms with the desired phenotype will survive and be... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog PostPublishedNov. 5, 2019, 1:28 p.m....different organisms and engineered Cas9 variants that were able to target different PAM sites (NGG, NGAN, NGNG... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....processing machinery present in almost every living organism. Precursor, or pre-tRNAs are cleaved by RNase ... illustrated the power of polycistronic gene organization, and its translation to CRISPR/Cas9 shows great... -
RNA Extraction Without A Kit
TypeBlog PostPublishedMarch 24, 2020, 1:15 p.m....proteins are retained in the interphase and bottom, organic layer. The top aqueous layer is extracted and RNA...layer to avoid carryover of DNA from the bottom, organic layer. Another trick is to precipitate the RNA ... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog PostPublishedMarch 3, 2020, 2:15 p.m....been used to construct plasmids for use in model organisms such as E. coli and yeast. Because this approach...2019. This novel tool is practical in cell and organ culture, as well as cell lineage studies. Eisenhoffer... -
Transferable Skills Guide: Cross-team Communication
TypeBlog PostPublishedNov. 21, 2019, 1:53 p.m....viral services. After a month or so of meetings, I began to notice something: even though our scientists ...sample,” did the word convey the same concept to Morgan, our senior engineer? The answer to these questions... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog PostPublishedApril 23, 2018, 2 p.m.... binding domains fused to either the hER or hPR ligand binding domains and the MSN2 transactivation domain...Transcription factor activation via the appropriate ligand - estradiol for hER or progesterone for hPR - leads... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m....understanding of the physiology and pathology of the basal ganglia in movement disorders. References and Resources...protein-coupled receptors potently activated by an inert ligand. Proceedings of the National Academy of Sciences...