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Showing: 221 - 240 of 694 results

  1. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...This post was contributed by guest blogger Eleanor Wang, a Research Specialist in Patrick Hsu's lab at...continue my scientific endeavors. I hope that this post can provide some perspective and advice to any undergraduate... you. Be proactive Rather than applying only to posted job offerings, the best research experiences typically... offer application fee waivers. One of the blog posts that helped me quite early on was Christine Liu’...graduate and/or undergraduate classes Postdoctoral researcher (postdoc) Getting more scientific and professional...or 9-to-5 job. I had to ask to find out what a “postdoc” is, and that “PI” stood for principal investigator...admin duties Staff scientist: similar role to a postdoc but more permanent Visiting scholar/student: visiting...

  2. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ... sound almost too good to be true! In a previous post, we covered systemic capsids, which allow AAVs to...tissues like the central nervous system. In this post, we’ll dive into the opposite: AAV serotypes with...ll cover the most commonly used serotypes in this post. However, tissue tropism varies both between species...AAV2-retro) We’ve covered AAVrg in a previous blog post, but to summarize briefly: AAVrg is derived from...

  3. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    Published
    Sept. 5, 2023, 1:15 p.m.
    ...sections, including our popular "Dimers and Multimers" post. It's also been re-organized and updated with a ...

  4. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...virus experiment. Subscribe to Viral Vector Blog Posts from Addgene Need more information on viral vectors...

  5. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...which contains the viral genome. In today’s blog post, we’ll focus on the AAV transfer plasmid and take...and combinations out there not discussed in this post, you’ve got the knowledge to be able to design a...WPRE The Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) forms a tertiary...

  6. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...) and subject age at time of injection. How long post-injection did you wait until performing the experiment...process of designing your experiment, read our blog post on important considerations when using AAVs. Experimental...

  7. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...for our first post, I thought it best to define language you’ll see throughout the posts and introduce...field, often through manuscripts, seminars, or posters. It is important to note that it is the actual ...

  8. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...using CRISPR/Cas9 RNPs? Check out this post Check Out Other Blog Posts on CRISPR Additional Resources on ...

  9. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ... This post was contributed by guest blogger Jack Leeming Editor of Naturejobs, part of Nature magazine...Resources on the Addgene Blog Check out our blog posts on science communication Find interviews scientists...

  10. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...This post was contributed by guest blogger Sean Stacey who recently attended both online and on site ...publications. My classmates consisted of graduate students, post-grads, and early career scientists. Over the course...

  11. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...vector educational resources as well, with both posts on the blog and pages on the Addgene website. blog.addgene.org...virus. You can find all of our viral vector blog posts here. If you have virus expertise you’d like to ...

  12. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...This post was contributed by guest blogger Meghan Monroy, a graduate student in Protein Science at the...on Your Plasmid Background with our Plasmids 101 Posts Resources on Addgene.org Find Empty Vectors for...

  13. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...graduate student in the Roth lab, for editing the blog post. References Olsen RHJ, DiBerto JF, English JG, Glaudin...blog Browse all of our fluorescent protein blog posts Find other fluorescent biosensors Resources on ...

  14. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...For more details on this technology, see our blog post Overview of AAV Encoded Calcium Sensors). Plasmid...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral ...

  15. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    Published
    Nov. 12, 2019, 2 p.m.
    ...springs My husband, Paul Blainey, at that time a post doc in the Quake Lab, volunteered to do the sampling... other Cas proteins Read all of our CRISPR blog posts Read about GeoCas9, another thermostable Cas9 Resources...was wonderful to learn that one project led by postdoc Dr. Stephanie Schmidt not only produced partially...

  16. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ... THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED...Fidelty Cas9 Mutants Browse All Genome Engineering Posts Additional Resources on Addgene.org Check Out ...

  17. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...communication skills. So, where can a graduate student or post-doc start? The good news is there are more opportunities...science on the one you already have. Read my earlier post about Scientists on Twitter here. 4)   If you enjoy...

  18. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ... Plasmids 101 blog posts Learn more about the origin of replication Find blog posts about plasmid cloning...maintained in one bacterial cell. In this Plasmids 101 post, we’ll cover why this happens, how it might affect...

  19. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...Professor at Georgetown University, contributed to this post. Adeno-associated viruses (AAVs) make fantastic...enhance the efficiency of genome editing. In this post we'll walk through a variety of ways you can use...many applications of AAV in some of our other blog posts and have also been able to browse some of the ready... al. "CRISPR/Cas9-mediated genome editing via postnatal administration of AAV vector cures haemophilia...

  20. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...chemotherapeutics, and ultraviolet light. In this post, we will describe the mechanism of homology directed... than 10 bp away if possible. Check out our blog post on Improving HDR efficiency for more details on ...highest frequency of HDR edits.  Check out our blog post on Easi-CRISPR for more details. References and...single-stranded DNA templates. Jacobi SA, et al. Poster at Keystone Precision Genome Engineering. 2017....
Showing: 221 - 240 of 694 results