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Addgene
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  2. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...quickly.  Through the years, we have perfected processes to curate the data our depositors share and enrich...and handle millions of queries. In an iterative process driven by our user experience experts, our software...

  3. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...analyze plasmids, which can help you visualize this process, or visit our restriction analysis blog post. ...now use next-generation sequencing during our QC process. This way, we can confirm the sequence of the entire...

  5. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...protein expression. Methylases involved with these processes (for example Dam and Dcm methylases) are independent...used for site directed mutagenesis. During this process, incorporation of a desired mutation into your ...

  6. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...understand, and the complicated and unique metabolic processes of plants are being investigated on limited funding... developmental, metabolic, and photosynthetic processes. Often the tools developed for plant molecular...

  7. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ... see how those mutations influence the disease process. In the Cell Stem Cell paper, for example, we generated... increase the efficiency of the genome editing process. The first really popular tool was the zinc finger...

  8. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...meant facilitating the Material Transfer Agreement process, which was a major barrier for depositors and requestors... requestors alike. We transformed this process by aligning institutions with standardized agreements and...

  10. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...your ideas, invite others into decision making processes, and even interview better! As a scientist turned..., re-oriented, continued presenting. The whole process took just under a minute. I can't say the journal...

  11. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...you with some advice on the antibody selection process. Antibodies are not one size fits all When choosing...experiments are the same. Differences in sample processing, fixation, antigen-retrieval, and permeabilization...

  13. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...still express ccdB and therefore would die. This procedure dramatically reduces the number of clones that...recombinant plasmid and therefore makes the cloning process much more efficient, as one does not have to thoroughly...

  14. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ... massively parallel combinatorial genetics." Proceedings of the National Academy of Sciences 111.34 (2014... CRISPR-Cas9 screening enabled by CombiGEM." Proceedings of the National Academy of Sciences113.9 (2016...

  15. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ... she could “get exposure to the drug discovery process.” The ClonTracer library originally came about ...that these are selected for during the treatment process. The authors went on to show that combination treatment...

  17. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...self-propagation by reproduction. Both physiological processes are controlled by deliberate and compulsory actions...goals is to promote an understanding of how these processes are regulated at the cellular, tissue and organ...

  18. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...important roles in many cellular and regulatory processes, but much of their functionality is yet to be ... of microRNA lead to its depletion and Drosha processing retardance. RNA Biol. 2014;11(10):1243-1249. ...

  19. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...interference (DIC) microscopy and computer imaging processing. The combination of these methods provides unique...transgene control in Caenorhabditis elegans." Proceedings of the National Academy of Sciences 115.15 (2018...

  20. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ... the nucleotide sequence during the DNA repair process. The addition of small molecules that promote homologous...Also, because non-homologous repair is a random process, zygotes edited using the same nuclease may not...
Showing: 221 - 240 of 515 results