...Information Science and Technology 60.4 (2009): 858-862. 2. Van Noorden, Richard. "Interdisciplinary research...Librarianship and Scholarly Communication 2.2 (2014): 2. Additional Resources on the Addgene Blog 6 Tools...others for failing to find our work?  I study processes for diagnosing and resolving organizational performance...impact of their contribution. It offers a 5-step process and several examples for creating effective research...

...citizens enter into in order to protect their rights (2). Less formally, governments are the organizations...science education and literacy that this changes.  2) Lumping all scientists (and consequently their results...politics? Science is an apolitical process for seeking knowledge. The process begins with a testable hypothesis...hypotheses and the cycle begins again. No part of this process is inherently political so why is there a march...scientists as well as other citizens. Thus civilization proceeds in an informed fashion. For science to be useful...

...faster when using a dual guide system.   Fig. 2: Size screening for large deletion events.   ... Biol 17: https://doi.org/10.1186/s13059-016-1012-2 Find more CRISPR Resources at Addgene: Read our CRISPRGuide...looking for, you are ready to begin the screening process. Want to set yourself up for a screening success...then a single, bulk validation may be enough to proceed with your experimental plan. Validating knockout...

...Halloween is only 2 days away and here at Addgene we couldn’t be more excited! As a small, tight-knit...suggestions for this year’s teams that may be procrastinating choosing their costume?  As our organization...

...First, the light-responsive molecules of cryptochrome 2 are the chromophores flavin-adenine dinucleotide and...of optogenetic control of endogenous cellular processes." The LITE system can now be applied to unraveling...genetic and epigenetic players in normal biological processes and disease states. While LITE plasmids have clear...

...to a specific area at the plasma membrane (Figure 2). Just express the plasma membrane-localized iLID ...cells will be ready for the spotlight.    Figure 2: Opto-RhoGEFs to control Rho GTPase activity. A) Schematic...great way to stimulate and study these physical processes. Joachim Goedhart and colleagues in the Gadella...could be applied to study other actin-dependent processes in cells and tissues. And, this Opto-RhoGEF design...the protein of interest supports a variety of processes in different cell types. One great way to do this...

...would label everything but the CD8+ T cells (Figure 2). Even better, we can use the same fluorophore for...gating on the negative population.     Figure 2: Dump gating labels all unwanted cell lineages with... for entry of the antibodies. Here, a two-step process is employed: the cells are first fixed and then...phosphatases, and ubiquitin ligases; and many more. This process, however, leaves the nucleus intact. If markers...not need to use a separate fix/perm cytosolic procedure in that case.     Figure 3: For intracellular...

...linear region to solve for total expression time. 2. Timer functionality with a single gene Although it...290.5496 (2000): 1585-1588. PubMed PMID: 11090358. 2. Tsuboi, Takashi, et al. "Age-dependent preferential...allowing them to optimize the compound production process. How have you used FP timers? Are you planning ...

...your sample (i.e. cultured cells or tissues).  Step 2: attach anchors to your sample that will link biomolecules...quite so much. Created with biorender.com Steps 2-4 are not found in standard IHC/ICC protocols and ....)  Step 5: Image   Figure 1: The ExM process allows you to physically magnify a sample so you...

...of these neurons via the channel Channelrhodopsin 2 (ChR2) reduced glutamate transmission from the mPFC...deliver Cre-dependent expression of  Channelrhodopsin 2 (ChR2), a modified receptor protein that activates...tracking software. Locomotion is a highly complex process on the level of individual neurons and in terms...

... a western, proteins are: (1) separated by size, (2) transferred to a membrane, and  (3) detected using...expensive system-specific sandwiches.   Figure 2: The western blot dry apparatus includes a stack of...gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences...  Figure 1: Overview of the Western blot process. The sample, a mixture of protein is separated ...antibodies, and finally visualized.   This process allows you to detect a single, specific protein...conduct the electric current. The wet transfer process is very effective in transferring a broad range...common readout for subcellular fractionations, a procedure that isolates proteins from different cellular...

...bringing in environmental contaminants.  Figure 2: An Addgenie works in the tissue culture hood while...doi.org/10.1290/1071-2690(2002)038<0086:caeomi>2.0.co;2 Uphoff CC, Drexler HG (2011) Detecting Mycoplasma ... enter the culture medium during subculturing processes or from the air if the cell line remains exposed... infecting mycoplasma. The primers used in the process are either species/gene-specific or universal. .... Some amount of expertise is required in this process because result interpretation might be difficult...by training eligible staff for the cell culture process and hence eliminate one of the causes of contamination...filtration. There is no 100% assurance that this process can avoid contamination but it’s a very good practice...

...Chapter 1 - Who are Addgene's Founders? Chapter 2 - The process of founding Addgene, where the idea for Addgene...

...Almo, Institute for Protein Innovation  1:18:45 - 2:01:18 (end) - Panel Alex Tucker (Ginkgo Bioworks)...their curation of citable, linkable figures. Cause #2: Hidden “negative” data We all want to be able to ...doing an experiment wrong. However, even simple procedures like pouring a plate or running a gel have intricacies... retain searchable, thorough notes on how lab procedures were actually performed, potentially curtailing... sense on many different levels - it’s hard to process large amounts of information and, from a SciComm...how and why papers change throughout the review process. This will hopefully push reviewers towards more...experimental design as part of the publication process (Nosek et al 2018). In this ideal situation, a ...

...phosphoinositides (Varnai and Balla, 2008) (Figure 2). RNA production can be detected by using a MS2 coat... 296.5569 (2002): 913-916. PubMed PMID: 11988576. 2. Tsien, Roger Y. "The green fluorescent protein." ...for biological spectroscopy and microscopy." Proceedings of the National Academy of Sciences 114.23 (2017... real-time multicolor luminescence imaging." Proceedings of the National Academy of Sciences (2015): 201418468...

...tagging." Nature methods (2015). PubMed PMID:26322837. 2. Stadler, Charlotte, et al. "Immunofluorescence and...the attributes of the native protein. Since this process takes a lot of time and energy, Martí Aldea and...

...Transgenesis." PLoS ONE. 8(12): e83043 (20 December 2013). 2. Carola Engler et al. "A One Pot, One Step, Precision...contributing more modules into the public domain in the process. “We had several motivations for making GreenGate...

...having trouble preparing your most excellent resume. 2) Follow job boards and check them regularly Use your...existing relationships as part of the job application process can dramatically increase the chances of being ...document. 6) Use your network! During the entire process, use whatever means you can to get your resume ...

...two options to begin your plasmid deposit. Figure 2: Choose to submit plasmid(s) online or by using a ...format. Figure 6: Add sequence, maps, and files. Step 2: Gene and Insert *Note: This page will not appear ...starting the process. After you have logged in to your account, you can begin the deposit process by clicking... as a step-by-step guide to the online deposit process. To deposit plasmids online, you will need to create...the Deposit menu: Figure 1: Begin the deposit process by clicking "Submit Online Deposit" or "Download...published in a peer-reviewed journal. To start this process, simply enter your preprint title or a general ...Hold for publication: we will initiate our MTA process and perform standard quality control but will not...

... 1: Genetically Engineered Mice and Part 2: Breeding and Crossing Mice. My first CRISPR mouse experiment...is less predictable. In our best case, we saw that 2 out of 3 mice carried a 5 kb insertion in the ROSA...not always guaranteed. To learn more about this process check out our Mouse Modeling blog posts: Part 1...have germline competent ESC lines. Second, the process is much quicker. It takes 3 months to generate ...