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We narrowed to 521 results for: she

Showing: 221 - 240 of 521 results

  1. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...Lumio Green and Lumio Red from Invitrogen (now ThermoFisher Scientific). These reagents are nonfluorescent...is that binding is covalent, so free dye can be washed away. In addition, labeling contrast can be improved... developed, so don’t be afraid to try novel, unpublished combinations of labeling strategies. FRET experimental...

  2. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...around the same time. (The papers were actually published in the same journal on the same day.) This team...lot of terminology in this post! Here’s a quick refresher if you ever lose track of which acronym is which...10.1038/s41556-023-01342-2 Koblan, L. W., Arbab, M., Shen, M. W., Hussmann, J. A., Anzalone, A. V., Doman,...

  3. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...CRISPR editing world. The approach, originally published in Nature, is a “search-and-replace” genome editing...Yang, W., Leith, A., Daza, R. M., Martin, B., & Shendure, J. (2022). Precise genomic deletions using paired...s41587-021-00868-w Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A., Chen, P. J., Anzalone, ...

  4. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...optimized primers that may work better than the published ones, so if/when you use HercII or Kapa Hifi the...all FAQs from Le Cong on June 24, 2025 by Alyssa Shepard.   References and Resources References Beumer...Additional resources on the Addgene blog CRISPR Cheat Sheet Build Your CRISPR Vocabulary CRISPR 101: Off-Target...

  5. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...transcription start site. In STARR-seq, randomly sheared genomic libraries are cloned downstream of a minimal...simple and complex DNA constructs Have you ever wished you could design a gene knockdown or overexpression...characterize positive hits. This work has been published in Cell. The Moffat laboratory maintains a website...Center has kindly constructed and deposited an unpublished set of Zika gene plasmids to Addgene’s repository...

  6. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...to Cas9. All of this is accompanied by new or refreshed figures made using BioRender.com. Figure... Alyssa Shepard...

  7. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    Published
    May 12, 2017, 12:58 p.m.
    ...you ever thought - Is there an easy way to find published plasmids? Do you spend a lot of time in PubMed...to set-up emails to alert you when papers are published on your favorite topic. If you have any other...

  8. New Viral Vectors - March 2024

    Type
    Blog Post
    Published
    March 5, 2024, 2:15 p.m.
    ... serotype pAAV-hDlx-Flex-dTomato-Fishell_7 AAV2 Controls Fishell New serotype pAAV-mDlx-NLS-mRuby2...

  10. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...In the late 1980s, another researcher, Douglas Prasher, got the idea that this new green glowing protein...reported its sequence. Soon after that, in 1994, Prasher’s collaborator Martin Chalfie expressed GFP in exogenous...

  11. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ...labels! Reusing plastic Historically, scientists washed and reused materials such as glass pipettes or ...some of these plastics are high grade and can be washed and reused. This might sound risky in terms of ...

  12. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...Since the first research applications of GFP were published in the 1990s, biologists have spent a lot of time...proteins for your imaging needs. If you need an FP refresher, see our FP blog posts.     References Sattarzadeh...

  13. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ... of Human Life at the MOS. (If you want a fun refresher on the Central Dogma of Biology, I would stop ...cocktail hour, we feasted on sliders, hot dogs and a mashed potato bar (complete with all the fixings), as ...

  14. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...that there’s a new CRISPR advance or technique published every week! One of the newest applications is ...fluorescent distances correlating with the previously established physical map. In comparing pairs of targets ~...

  16. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ... to keep at your desk? We’ve got a CRISPR cheat sheet for that! Resources Further reading Wang, J. Y....Addgene's CRISPR 101 eBook  Addgene's CRISPR Cheat Sheet  Resources on the Addgene blog A Needle in a Base-Stack...

  17. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...stabilize DNA-protein interactions for subsequent sheering of DNA by sonification. Though it helps capture... your own lab Very recently, the Henikoff lab published a preprint paper outlining improvements to the...

  18. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...Viruses are simple: a genome packaged in a protein shell (Taylor, 2014). They’re so simple that we can’t ...While all viruses have a genome inside a protein shell known as a capsid, some also have a lipid bilayer...
Showing: 221 - 240 of 521 results