We narrowed to 387 results for: abo.5

... raising salaries as their website implies. These 5 organizations, according to the NSF data, account ...the Addgene Blog How did this change in labor laws come about? In March 2014, the White House issued a...must be above this level, and certain job duty tests must be met. In the Department of Labor’s general... to $47,476 per year, under updates to the Fair Labor Standards Act (FLSA). This is having a major effect...ensuring that either all affected scientists are paid above this threshold or for tracking the hours that these... a memorandum to the Secretary of Labor to update the FLSA, which had not seen revisions since 2004. In... In July 2015, the Department of Labor issued a Notice of Proposed Rulemaking, soliciting feedback by ...

...more important to start early. Think about your plan for the next 5 years. Does an academic career work...patent application processes? How about facilitating a collaboration between research groups? Perhaps ... a good job summarizing what a resume should be about. With a CV, information is usually presented chronologically...developed through your research? Don't simply write about the research you did. Emphasize the skills. If you... structures, then you might know a thing or two about computer modeling. Make sure to focus on these skills...the job you are applying for, in which case talk about your research, as an understanding of the topic ...you some advantage over other candidates. Write about your transferable skills Some of the skills you ...

...focusing on a task, like writing, and then take a 5-minute break. Each 30-minute interval is called a ...frequency of use in black. This paper talks more about the development of the De-jargonizer.   Academic...with others which makes it easier to work with collaborators. Zotero is a desktop based program that’s compatible...episode 2 "Organizing Literature" : I found out about Zotero here Dear Grad Student podcast episode 2 ...Programming, & Productivity": This podcast talks about Mendeley, Zotero, and other citation managers and...the pomodoro technique": This podcast talks more about using the Pomodoro method in grad school Additional...

...Elsevier BV. https://doi.org/10.1016/s0021-9258(18)99982-5 More resources on the Addgene blog Antibodies 101:...flexibility needed for multiplexing. In the case above, you can’t costain with your desired antibodies ...multiplexing with chimeric antibodies. In the case above you should use an anti-rat IgG1 secondary antibody...this blog post has answered all of your questions about the utility of isotype conversions and chimeric ..., Bright, S. M., Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R., King, D. J., Norris, S. H., &...

...Make a stock solution of 2.5 mM biotin (50X) because 5 mM is barely soluble at RT. Track expression and purification...Resources at Addgene.org Learn about Popular Plasmids in the Repository Learn about New Plasmid Technologies...

...emission maximum is 675 nm. mCarmine is monomeric, 4-5 fold brighter than mNeptune684, and has a favorable... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.   ...emission maxima toward the red end of the spectrum (above 600 nm) and increasing the brightness. Arne Fabritius...

... C Stringent pACYC ~10 p15A B Relaxed pSC101 ~5 pSC101 C Stringent pBluescript ~300-500 ColE1 (derivative...last updated Nov 10, 2020. Now that we know all about antibiotic resistance genes, let’s consider another...the replicon. For example, the pMB1 ori maintains about 20 copies per cell, while pUC – which differs by...

... Cell Envelope. Cold Spring Harb Perspect Biol. 2(5) a000414 (2010). Cleveland Clinic. Gram Stain. https... it can be used in a lab? Here we’ll be talking about the ins and outs of this classification system, ...The classification system is also useful in a laboratory setting. When designing a plasmid to be inserted...

...fluorescence, as the GCaMP7f variant, and is approximately 5-fold brighter than GCaMP6s. jGCaMP7f is the fastest...brightness after ~100 action potentials. This is about twice as bright as GCaMP7f and GCaMP7s. See all...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

...This is the fourth in a 5 part series on Management for scientists. Once you are responsible for managing...Medical School researcher Connie Cepko to learn about her management style. Recipes for delegation success...accountability? What types of issues do you want to know about right away? Provide adequate support. The manager...

...Syntaxin3 gene was the winner. We used the next 4-5 weeks to work on cloning the Syntaxin3 gene in a pLenti...intern coordinator shortly after hearing her talk about Addgene’s internship opportunity taking place next...

...Biotechnology, 1–13. https://doi.org/10.1038/s41587-023-01833-5 Resources on Addgene.org Optogenetics guide Chemogenetics...I often wonder about the gut-brain axis (admittedly for self-serving reasons – I want to understand my...

...KA, Dowdy SF. Trends Cell Biol. 2000 Jul;10(7):290-5. PubMed. Is VP22 nuclear homing an artifact? Lundberg... a protocol has been established) may be less laborious than viral purification, and it does not raise...

...digested with either HindIII and BamHI alone (lanes 4-5), there is a single band of 7.3 kb representing the...1kb ladder (lane 1) that has bands ranging from about 500bp to 10kb, with the 3.0kb fragment having increased...

...form it’s chromophore at a more comfortable 37°C (5). Dendra2 photoconverts from green to red fluorescence...15910. PubMed PMID:15505211. PubMed PMCID:PMC528746. 5.Gurskaya, Nadya G., et al. "Engineering of a monomeric...emitted, making it easier to capture a clear image above background. The majority of PA-FPs come in 2 flavours...Additional Resources on the Addgene Blog Read More about Fluorescent Protein Imaging Choosing Your Fluorescent...

... entering the specifics of your plasmids. Figure 5: Click the "Enter Data" button to add your plasmid...Enter the growth and distribution information. Step 5: Verification On this page you will see a preview ...annotate on this page. Figure 7: Enter information about the gene/insert(s). Step 3: Cloning Information ...

...who knows about you. I found some other great tips for finding mentors in this recent blog 5 Strategies...work if both parties are diligent about replying and thinking about good questions and answers. Professional...Medical School researcher Connie Cepko to learn about her mentoring style. Mentors at home and away We...Catalyst Report “Making Mentoring Work” to learn about what makes a good mentoring program. If your organization...much less threatening! If they say no, don’t worry about it. It is a compliment to ask someone to share their...

...Labs plasmid grant we are excited to introduce the 5 awardees and their research. These researchers span...Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources, equipment, and...discovery, and synthetic biology. Read on to learn more about the five awardees and their promising research projects... to the Addgene blog for updates and future collaborations. Many thanks to all of the awardees, all who...Additional Resources on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant Check Out...Expression Systems Synthetic Biology Resources Learn about Global Plasmid Distribution ...

...nano-lantern emitted yellow-green light and exhibited 3-5 fold increased brightness over previous BRET probes...Since their first publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns...Nano-lanterns, Takai et al. continuously imaged cells for about 4 hours! The latest enhanced Nano-lanterns (eNL)... Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job characterizing Nano-lanterns...Additional Resources on the Addgene Blog Learn about Photoactivatable Fluorescent Proteins Check Out ...

...can break down a dye-linked substrate called x-gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside) into...antibiotic selection here at Plasmids 101, we can talk about an even more specific method of screening your cloning... plasmid is a great start when cloning, but how about being able to choose those that contain plasmid ... a widely used method to do just that! (To read about other screening strategies, check out our screening... called α-complementation. The system described above was put to practical use in the following way. Scientists...